PERFORM Publications

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Title		Authors	PubMed ID
1	Using Models to (Re-)Design Synthetic Circuits.	McCallum G, Potvin-Trottier L	33405217 BIOLOGY
2	Computer-Aided Design of Active Pseudoknotted Hammerhead Ribozymes.	Najeh S, Zandi K, Djerroud S, Kharma N, Perreault J	32712917 ENCS
3	Metabolomic and lipidomic analyses of chronologically aging yeast.	Richard VR, Bourque SD, Titorenko VI	25213255 BIOLOGY
4	A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion.	Karim MA, Samyn DR, Brett CL	30317513 BIOLOGY
5	Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.	Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL	30317518 BIOLOGY
6	Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics.	Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA	28417376 CSFG
7	Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry	Robert J Gruninger	28417377 CSFG
8	Introduction: Overview of Fungal Genomics.	de Vries RP, Grigoriev IV, Tsang A	29876804 CSFG
9	Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing.	Bellemare A, John T, Marqueteau S	29876805 CSFG
10	Mass Spectrometry-Based Proteomics	Marcos Rafael Di Falco	29876812 CSFG
11	Evolutionary Adaptation to Generate Mutants.	de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I	29876815 BIOLOGY
12	Manual Gene Curation and Functional Annotation.	McDonnell E, Strasser K, Tsang A	29876819 CSFG
13	Evaluating Programs for Predicting Genes and Transcripts with RNA-Seq Support in Fungal Genomes.	Reid I	29876820 CSFG
14	Phylogenetic Analysis of Protein Family.	Song L, Wu S, Tsang A	29876824 CSFG

Title:	Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.
Authors:	Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL
Link:	https://www.ncbi.nlm.nih.gov/pubmed/30317518?dopt=Abstract
DOI:	10.1007/978-1-4939-8760-3_24
Publication:	Methods in molecular biology (Clifton, N.J.)
Keywords:	Cryogenic electron microscopy (cryoEM); Hemifusion; Lipid bilayer merger; Lysosome; Membrane fusion; SNARE; Tomography; Transmission electron microscopy (TEM); Vacuole;
PMID:	30317518	Category:	Methods Mol Biol	Date Added:	2019-06-07
Dept Affiliation:	BIOLOGY 1 Department of Biology, Concordia University, Montréal, QC, Canada. 2 Montreal Neurological Hospital and Institute, McGill University, Montréal, QC, Canada. 3 Facility for Electron Microscopy Research, Department of Anatomy and Cell Biology, McGill University, Montréal, QC, Canada. 4 Department of Biology, Concordia University, Montréal, QC, Canada. christopher.brett@concordia.ca.

Description:

Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.

Methods Mol Biol. 2019;1860:361-377

Authors: Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL

Abstract

SNARE-mediated membrane fusion is required for membrane trafficking as well as organelle biogenesis and homeostasis. The membrane fusion reaction involves sequential formation of hemifusion intermediates, whereby lipid monolayers partially mix on route to complete bilayer merger. Studies of the Saccharomyces cerevisiae lysosomal vacuole have revealed many of the fundamental mechanisms that drive the membrane fusion process, as well as features unique to organelle fusion. However, until recently, it has not been amenable to electron microscopy methods that have been invaluable for studying hemifusion in other model systems. Herein, we describe a method to visualize hemifusion intermediates during homotypic vacuole membrane fusion in vitro by transmission electron microscopy (TEM), electron tomography, and cryogenic electron microscopy (cryoEM). This method facilitates acquisition of invaluable ultrastructural data needed to comprehensively understand how fusogenic lipids and proteins contribute to SNARE-mediated membrane fusion-by-hemifusion and the unique features of organelle versus small-vesicle fusion.

PMID: 30317518 [PubMed - indexed for MEDLINE]

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