Keyword search (4,163 papers available)

"Methods Mol Biol" Category Publications:

Title Authors PubMed ID
1 Using Models to (Re-)Design Synthetic Circuits. McCallum G, Potvin-Trottier L 33405217
BIOLOGY
2 Computer-Aided Design of Active Pseudoknotted Hammerhead Ribozymes. Najeh S, Zandi K, Djerroud S, Kharma N, Perreault J 32712917
ENCS
3 Metabolomic and lipidomic analyses of chronologically aging yeast. Richard VR, Bourque SD, Titorenko VI 25213255
BIOLOGY
4 A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion. Karim MA, Samyn DR, Brett CL 30317513
BIOLOGY
5 Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy. Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL 30317518
BIOLOGY
6 Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics. Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA 28417376
CSFG
7 Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry Robert J Gruninger 28417377
CSFG
8 Introduction: Overview of Fungal Genomics. de Vries RP, Grigoriev IV, Tsang A 29876804
CSFG
9 Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing. Bellemare A, John T, Marqueteau S 29876805
CSFG
10 Mass Spectrometry-Based Proteomics Marcos Rafael Di Falco 29876812
CSFG
11 Evolutionary Adaptation to Generate Mutants. de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I 29876815
BIOLOGY
12 Manual Gene Curation and Functional Annotation. McDonnell E, Strasser K, Tsang A 29876819
CSFG
13 Evaluating Programs for Predicting Genes and Transcripts with RNA-Seq Support in Fungal Genomes. Reid I 29876820
CSFG
14 Phylogenetic Analysis of Protein Family. Song L, Wu S, Tsang A 29876824
CSFG

 

Title:Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry
Authors:Robert J Gruninger
Link:https://pubmed.ncbi.nlm.nih.gov/28417377/
DOI:10.1007/978-1-4939-6899-2_22
Publication:Methods in molecular biology (Clifton, N.J.)
Keywords:CAZy;  Exo-proteome;  Fungi;  Lignocellulose;  Mass spectrometry;  Proteomics; 
PMID:28417377 Category:Methods Mol Biol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Ave. South, Lethbridge, AB, Canada, T1J 4B1. robert.gruninger@agr.gc.ca.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada.
3 Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Ave. South, Lethbridge, AB, Canada, T1J 4B1.

Description:

Fungi utilize a unique mechanism of nutrient acquisition involving extracellular digestion. To understand the biology of these microbes, it is important to identify and characterize the function of proteins that are secreted and involved in this process. Mass spectrometry-based proteomics is a powerful tool to study complex mixtures of proteins and understand how the proteins produced by an organism change in response to different conditions. Many fungi are efficient decomposers of plant cell...




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