PERFORM Publications

Keyword search (4,163 papers available)

Title		Authors	PubMed ID
1	Using Models to (Re-)Design Synthetic Circuits.	McCallum G, Potvin-Trottier L	33405217 BIOLOGY
2	Computer-Aided Design of Active Pseudoknotted Hammerhead Ribozymes.	Najeh S, Zandi K, Djerroud S, Kharma N, Perreault J	32712917 ENCS
3	Metabolomic and lipidomic analyses of chronologically aging yeast.	Richard VR, Bourque SD, Titorenko VI	25213255 BIOLOGY
4	A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion.	Karim MA, Samyn DR, Brett CL	30317513 BIOLOGY
5	Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.	Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL	30317518 BIOLOGY
6	Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics.	Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA	28417376 CSFG
7	Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry	Robert J Gruninger	28417377 CSFG
8	Introduction: Overview of Fungal Genomics.	de Vries RP, Grigoriev IV, Tsang A	29876804 CSFG
9	Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing.	Bellemare A, John T, Marqueteau S	29876805 CSFG
10	Mass Spectrometry-Based Proteomics	Marcos Rafael Di Falco	29876812 CSFG
11	Evolutionary Adaptation to Generate Mutants.	de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I	29876815 BIOLOGY
12	Manual Gene Curation and Functional Annotation.	McDonnell E, Strasser K, Tsang A	29876819 CSFG
13	Evaluating Programs for Predicting Genes and Transcripts with RNA-Seq Support in Fungal Genomes.	Reid I	29876820 CSFG
14	Phylogenetic Analysis of Protein Family.	Song L, Wu S, Tsang A	29876824 CSFG

Title:	Manual Gene Curation and Functional Annotation.
Authors:	McDonnell E, Strasser K, Tsang A
Link:	https://www.ncbi.nlm.nih.gov/pubmed/29876819?dopt=Abstract
DOI:	10.1007/978-1-4939-7804-5_16
Publication:	Methods in molecular biology (Clifton, N.J.)
Keywords:	Gold-standard genome resource; Manual functional assignment; Manual gene curation; Whole-genome annotation;
PMID:	29876819	Category:	Methods Mol Biol	Date Added:	2019-06-07
Dept Affiliation:	CSFG 1 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada. 2 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada. kimchi.strasser@concordia.ca.

Description:

Manual Gene Curation and Functional Annotation.

Methods Mol Biol. 2018;1775:185-208

Authors: McDonnell E, Strasser K, Tsang A

Abstract

No genome sequencing project is complete without structural and functional annotation. Gene models and functional predictions for these models can be obtained relatively easily using computational methods, but they are prone to errors. We describe herein the steps we use to manually curate gene models and functionally annotate them. Our approach is to examine each gene model carefully, and improve its structure if necessary, using a comprehensive set of experimental and computational data as evidence. Then, functional predictions are assigned to the gene models based on conserved protein domains and sequence similarities. We use stringent sequence similarity cutoffs and reviewed sequence-database records as external sources for our annotations. By methodically choosing which evidence to use for each annotation, we minimize the risk of adopting and assigning false predictions to the gene models.

PMID: 29876819 [PubMed - indexed for MEDLINE]

BookR: School of Health Core Facilities Booking

"Methods Mol Biol" Category Publications:

Search Publications

No results