PERFORM Publications

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Title		Authors	PubMed ID
1	Using Models to (Re-)Design Synthetic Circuits.	McCallum G, Potvin-Trottier L	33405217 BIOLOGY
2	Computer-Aided Design of Active Pseudoknotted Hammerhead Ribozymes.	Najeh S, Zandi K, Djerroud S, Kharma N, Perreault J	32712917 ENCS
3	Metabolomic and lipidomic analyses of chronologically aging yeast.	Richard VR, Bourque SD, Titorenko VI	25213255 BIOLOGY
4	A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion.	Karim MA, Samyn DR, Brett CL	30317513 BIOLOGY
5	Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.	Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL	30317518 BIOLOGY
6	Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics.	Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA	28417376 CSFG
7	Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry	Robert J Gruninger	28417377 CSFG
8	Introduction: Overview of Fungal Genomics.	de Vries RP, Grigoriev IV, Tsang A	29876804 CSFG
9	Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing.	Bellemare A, John T, Marqueteau S	29876805 CSFG
10	Mass Spectrometry-Based Proteomics	Marcos Rafael Di Falco	29876812 CSFG
11	Evolutionary Adaptation to Generate Mutants.	de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I	29876815 BIOLOGY
12	Manual Gene Curation and Functional Annotation.	McDonnell E, Strasser K, Tsang A	29876819 CSFG
13	Evaluating Programs for Predicting Genes and Transcripts with RNA-Seq Support in Fungal Genomes.	Reid I	29876820 CSFG
14	Phylogenetic Analysis of Protein Family.	Song L, Wu S, Tsang A	29876824 CSFG

Title:	Evolutionary Adaptation to Generate Mutants.
Authors:	de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I
Link:	https://www.ncbi.nlm.nih.gov/pubmed/29876815?dopt=Abstract
DOI:	10.1007/978-1-4939-7804-5_12
Publication:	Methods in molecular biology (Clifton, N.J.)
Keywords:	Adaptation; Evolution; Mutants; Screening; Selection;
PMID:	29876815	Category:	Methods Mol Biol	Date Added:	2019-06-07
Dept Affiliation:	BIOLOGY 1 Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands. r.devries@westerdijkinstitute.nl. 2 Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands. 3 Centre for Structural and Functional Genomics, Department of Biology, Concordia University, Montreal, QC, Canada.

Description:

Evolutionary Adaptation to Generate Mutants.

Methods Mol Biol. 2018;1775:133-137

Authors: de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I

Abstract

In this chapter we describe a method to generate mutants of filamentous fungi using their genomic plasticity and rapid adaptability to their environment. This method is based on spontaneous mutations occurring in relation to improved growth of fungi on media by repeated inoculation resulting in adaptation of the strain to the condition. The critical aspect of this method is the design of the selective media, which will depend strongly on the phenomenon that will be studied. This method is advantageous over UV or chemical random mutagenesis as it results in a lower frequency of undesired mutations and can result in strains that combined with (post)genomic approaches can enhance our understanding of the mechanisms driving various biological processes. In addition, it can be used to obtain better strains for various industrial applications. The method described here is specific for sporulating fungi and has so far not yet been tested for nonsporulating fungi.

PMID: 29876815 [PubMed - indexed for MEDLINE]

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