Keyword search (4,163 papers available)

"Martin VJJ" Authored Publications:

Title Authors PubMed ID
1 Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield Narcross L; Pyne ME; Kevvai K; Siu KH; Dueber JE; Martin VJJ; 41779670
BIOLOGY
2 Tri-Functional CRISPR Screen Reveals Overexpression of em QDR2 /em and em QDR3 /em Transporters Increase Fumaric Acid Production in em Kluyveromyces marxianus /em Thornbury M; Omran RP; Kumar L; Knoops A; Abushahin R; Whiteway M; Martin VJJ; 41277095
BIOLOGY
3 Sequencing of a Dairy Isolate Unlocks em Kluyveromyces marxianus /em as a Host for Lactose Valorization Thornbury M; Knoops A; Summerby-Murray I; Dhaliwal J; Johnson S; Utomo JC; Joshi J; Narcross L; Remondetto G; Pouliot M; Whiteway M; Martin VJJ; 40629255
BIOLOGY
4 Functional and structural characterization of an IclR family transcription factor for the development of dicarboxylic acid biosensors Pham C; Nasr MA; Skarina T; Di Leo R; Kwan DH; Martin VJJ; Stogios PJ; Mahadevan R; Savchenko A; 38696354
BIOLOGY
5 Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies Husser MC; Pham NP; Law C; Araujo FRB; Martin VJJ; Piekny A; 38652106
BIOLOGY
6 Genome sequencing of 15 acid-tolerant yeasts Bagley JA; Pyne ME; Exley K; Kevvai K; Wang Q; Whiteway M; Martin VJJ; 37747226
BIOLOGY
7 Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid Pyne ME; Bagley JA; Narcross L; Kevvai K; Exley K; Davies M; Wang Q; Whiteway M; Martin VJJ; 37652930
BIOLOGY
8 CRAPS: Chromosomal-Repair-Assisted Pathway Shuffling in Yeast Dykstra CB; Pyne ME; Martin VJJ; 37584634
BIOLOGY
9 Divergent directed evolution of a TetR-type repressor towards aromatic molecules Nasr MA; Martin VJJ; Kwan DH; 37377432
BIOLOGY
10 Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera) Pyne ME; Gold ND; Martin VJJ; 37004909
BIOLOGY
11 Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ; 36416720
BIOLOGY
12 The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit Bean BDM; Whiteway M; Martin VJJ; 35708612
BIOLOGY
13 Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols Bean BDM; Mulvihill CJ; Garge RK; Boutz DR; Rousseau O; Floyd BM; Cheney W; Gardner EC; Ellington AD; Marcotte EM; Gollihar JD; Whiteway M; Martin VJJ; 35610225
BIOLOGY
14 A Versatile Transcription Factor Biosensor System Responsive to Multiple Aromatic and Indole Inducers Nasr MA; Timmins LR; Martin VJJ; Kwan DH; 35316041
CHEMBIOCHEM
15 Engineering Yeast for De Novo Synthesis of the Insect Repellent Nepetalactone Davies ME; Tsyplenkov D; Martin VJJ; 34748704
BIOLOGY
16 A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids. Pyne ME, Kevvai K, Grewal PS, Narcross L, Choi B, Bourgeois L, Dueber JE, Martin VJJ 32620756
BIOLOGY
17 Author Correction: Building a global alliance of biofoundries. Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS 31296848
CHEMBIOCHEM
18 An Engineered Aro1 Protein Degradation Approach for Increased cis,cis-Muconic Acid Biosynthesis in Saccharomyces cerevisiae. Pyne ME, Narcross L, Melgar M, Kevvai K, Mookerjee S, Leite GB, Martin VJJ 29934332
BIOLOGY
19 A Highly Characterized Synthetic Landing Pad System for Precise Multicopy Gene Integration in Yeast. Bourgeois L, Pyne ME, Martin VJJ 30372609
BIOLOGY
20 Microbial Factories for the Production of Benzylisoquinoline Alkaloids. Narcross L, Fossati E, Bourgeois L, Dueber JE, Martin VJJ 26775900
BIOLOGY
21 Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii Zhan Y; Marchand CH; Maes A; Mauries A; Sun Y; Dhaliwal JS; Uniacke J; Arragain S; Jiang H; Gold ND; Martin VJJ; Lemaire SD; Zerges W; 29481573
CSFG
22 Determinants of selection in yeast evolved by genome shuffling. Biot-Pelletier D, Pinel D, Larue K, Martin VJJ 30356826
CSFG
23 A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast. Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ 30474973
CSFG
24 Engineering Plant Secondary Metabolism in Microbial Systems. Pyne ME, Narcross L, Martin VJJ 30643013
CSFG
25 Building a global alliance of biofoundries. Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS 31068573
CHEMBIOCHEM

 

Title:A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.
Authors:Gold NDFossati EHansen CCDiFalco MDouchin VMartin VJJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/30474973?dopt=Abstract
DOI:10.1021/acssynbio.8b00470
Publication:ACS synthetic biology
Keywords:cytochrome P450 monooxygenasecytochrome P450 reductasediterpenoid biosynthesissteviol glucosides
PMID:30474973 Category:ACS Synth Biol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Centre for Applied Synthetic Biology , Concordia University , Montréal , Québec H4B 1R6 , Canada.
2 Plant Biochemistry Laboratory, Department of Plant and Environmental Science , University of Copenhagen , DK-1871 Frederiksberg C , Denmark.
3 Center for Synthetic Biology , University of Copenhagen , DK-1871 Frederiksberg C , Denmark.
4 Centre for Structural and Functional Genomics , Concordia University , Montréal , Québec H4B 1R6 , Canada.
5 Evolva , Lersø Parkallé 42-44 , DK-2100 Copenhagen Ø , Denmark.
6 Department of Biology, Centre for Structural and Functional Genomic , Concordia University , Montréal , Québec H4B 1R6 , Canada.

Description:

A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.

ACS Synth Biol. 2018 Dec 21;7(12):2918-2929

Authors: Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ

Abstract

Biosynthesis of steviol glycosides in planta proceeds via two cytochrome P450 enzymes (CYPs): kaurene oxidase (KO) and kaurenoic acid hydroxylase (KAH). KO and KAH function in succession with the support of a NADPH-dependent cytochrome P450 reductase (CPR) to convert kaurene to steviol. This work describes a platform for recombinant production of steviol glucosides (SGs) in Saccharomyces cerevisiae, demonstrating the full reconstituted pathway from the simple sugar glucose to the SG precursor steviol. With a focus on optimization of the KO-KAH activities, combinations of functional homologues were tested in batch growth. Among the CYPs, novel KO75 (CYP701) and novel KAH82 (CYP72) outperformed their respective functional homologues from Stevia rebaudiana, SrKO (CYP701A5) and SrKAH (CYP81), in assays where substrate was supplemented to culture broth. With kaurene produced from glucose in the cell, SrCPR1 from S. rebaudiana supported highest turnover for KO-KAH combinations, besting two other CPRs isolated from S. rebaudiana, the Arabidopsis thaliana ATR2, and a new class I CPR12. Some coexpressions of ATR2 with a second CPR were found to diminish KAH activity, showing that coexpression of CPRs can lead to competition for CYPs with possibly adverse effects on catalysis.

PMID: 30474973 [PubMed - in process]





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