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PubMed Publications| Keyword search (4,163 papers available) |  |
"Martin VJJ" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield | Narcross L; Pyne ME; Kevvai K; Siu KH; Dueber JE; Martin VJJ; | 41779670 BIOLOGY |
| 2 | Tri-Functional CRISPR Screen Reveals Overexpression of em QDR2 /em and em QDR3 /em Transporters Increase Fumaric Acid Production in em Kluyveromyces marxianus /em | Thornbury M; Omran RP; Kumar L; Knoops A; Abushahin R; Whiteway M; Martin VJJ; | 41277095 BIOLOGY |
| 3 | Sequencing of a Dairy Isolate Unlocks em Kluyveromyces marxianus /em as a Host for Lactose Valorization | Thornbury M; Knoops A; Summerby-Murray I; Dhaliwal J; Johnson S; Utomo JC; Joshi J; Narcross L; Remondetto G; Pouliot M; Whiteway M; Martin VJJ; | 40629255 BIOLOGY |
| 4 | Functional and structural characterization of an IclR family transcription factor for the development of dicarboxylic acid biosensors | Pham C; Nasr MA; Skarina T; Di Leo R; Kwan DH; Martin VJJ; Stogios PJ; Mahadevan R; Savchenko A; | 38696354 BIOLOGY |
| 5 | Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies | Husser MC; Pham NP; Law C; Araujo FRB; Martin VJJ; Piekny A; | 38652106 BIOLOGY |
| 6 | Genome sequencing of 15 acid-tolerant yeasts | Bagley JA; Pyne ME; Exley K; Kevvai K; Wang Q; Whiteway M; Martin VJJ; | 37747226 BIOLOGY |
| 7 | Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid | Pyne ME; Bagley JA; Narcross L; Kevvai K; Exley K; Davies M; Wang Q; Whiteway M; Martin VJJ; | 37652930 BIOLOGY |
| 8 | CRAPS: Chromosomal-Repair-Assisted Pathway Shuffling in Yeast | Dykstra CB; Pyne ME; Martin VJJ; | 37584634 BIOLOGY |
| 9 | Divergent directed evolution of a TetR-type repressor towards aromatic molecules | Nasr MA; Martin VJJ; Kwan DH; | 37377432 BIOLOGY |
| 10 | Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera) | Pyne ME; Gold ND; Martin VJJ; | 37004909 BIOLOGY |
| 11 | Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA | Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ; | 36416720 BIOLOGY |
| 12 | The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit | Bean BDM; Whiteway M; Martin VJJ; | 35708612 BIOLOGY |
| 13 | Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols | Bean BDM; Mulvihill CJ; Garge RK; Boutz DR; Rousseau O; Floyd BM; Cheney W; Gardner EC; Ellington AD; Marcotte EM; Gollihar JD; Whiteway M; Martin VJJ; | 35610225 BIOLOGY |
| 14 | A Versatile Transcription Factor Biosensor System Responsive to Multiple Aromatic and Indole Inducers | Nasr MA; Timmins LR; Martin VJJ; Kwan DH; | 35316041 CHEMBIOCHEM |
| 15 | Engineering Yeast for De Novo Synthesis of the Insect Repellent Nepetalactone | Davies ME; Tsyplenkov D; Martin VJJ; | 34748704 BIOLOGY |
| 16 | A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids. | Pyne ME, Kevvai K, Grewal PS, Narcross L, Choi B, Bourgeois L, Dueber JE, Martin VJJ | 32620756 BIOLOGY |
| 17 | Author Correction: Building a global alliance of biofoundries. | Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS | 31296848 CHEMBIOCHEM |
| 18 | An Engineered Aro1 Protein Degradation Approach for Increased cis,cis-Muconic Acid Biosynthesis in Saccharomyces cerevisiae. | Pyne ME, Narcross L, Melgar M, Kevvai K, Mookerjee S, Leite GB, Martin VJJ | 29934332 BIOLOGY |
| 19 | A Highly Characterized Synthetic Landing Pad System for Precise Multicopy Gene Integration in Yeast. | Bourgeois L, Pyne ME, Martin VJJ | 30372609 BIOLOGY |
| 20 | Microbial Factories for the Production of Benzylisoquinoline Alkaloids. | Narcross L, Fossati E, Bourgeois L, Dueber JE, Martin VJJ | 26775900 BIOLOGY |
| 21 | Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii | Zhan Y; Marchand CH; Maes A; Mauries A; Sun Y; Dhaliwal JS; Uniacke J; Arragain S; Jiang H; Gold ND; Martin VJJ; Lemaire SD; Zerges W; | 29481573 CSFG |
| 22 | Determinants of selection in yeast evolved by genome shuffling. | Biot-Pelletier D, Pinel D, Larue K, Martin VJJ | 30356826 CSFG |
| 23 | A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast. | Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ | 30474973 CSFG |
| 24 | Engineering Plant Secondary Metabolism in Microbial Systems. | Pyne ME, Narcross L, Martin VJJ | 30643013 CSFG |
| 25 | Building a global alliance of biofoundries. | Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS | 31068573 CHEMBIOCHEM |
| Title: | Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies | ||||
| Authors: | Husser MC, Pham NP, Law C, Araujo FRB, Martin VJJ, Piekny A | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/38652106/ | ||||
| DOI: | 10.7554/eLife.92819 | ||||
| Publication: | eLife | ||||
| Keywords: | cell biology; crispr; cytokinesis; endogenous tagging; gene editing; human; ipsc; live imaging; | ||||
| PMID: | 38652106 | Category: | Date Added: | 2024-04-23 | |
| Dept Affiliation: |
BIOLOGY
1 Biology Department, Concordia University, Montreal, Canada. 2 Center for Microscopy and Cellular Imaging, Concordia University, Montreal, Canada. 3 Center for Applied Synthetic Biology, Concordia University, Montreal, Canada. |
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Description: |
Endogenous tags have become invaluable tools to visualize and study native proteins in live cells. However, generating human cell lines carrying endogenous tags is difficult due to the low efficiency of homology-directed repair. Recently, an engineered split mNeonGreen protein was used to generate a large-scale endogenous tag library in HEK293 cells. Using split mNeonGreen for large-scale endogenous tagging in human iPSCs would open the door to studying protein function in healthy cells and across differentiated cell types. We engineered an iPS cell line to express the large fragment of the split mNeonGreen protein (mNG21-10) and showed that it enables fast and efficient endogenous tagging of proteins with the short fragment (mNG211). We also demonstrate that neural network-based image restoration enables live imaging studies of highly dynamic cellular processes such as cytokinesis in iPSCs. This work represents the first step towards a genome-wide endogenous tag library in human stem cells. |
