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PubMed Publications| Keyword search (4,163 papers available) |  |
"Martin VJJ" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield | Narcross L; Pyne ME; Kevvai K; Siu KH; Dueber JE; Martin VJJ; | 41779670 BIOLOGY |
| 2 | Tri-Functional CRISPR Screen Reveals Overexpression of em QDR2 /em and em QDR3 /em Transporters Increase Fumaric Acid Production in em Kluyveromyces marxianus /em | Thornbury M; Omran RP; Kumar L; Knoops A; Abushahin R; Whiteway M; Martin VJJ; | 41277095 BIOLOGY |
| 3 | Sequencing of a Dairy Isolate Unlocks em Kluyveromyces marxianus /em as a Host for Lactose Valorization | Thornbury M; Knoops A; Summerby-Murray I; Dhaliwal J; Johnson S; Utomo JC; Joshi J; Narcross L; Remondetto G; Pouliot M; Whiteway M; Martin VJJ; | 40629255 BIOLOGY |
| 4 | Functional and structural characterization of an IclR family transcription factor for the development of dicarboxylic acid biosensors | Pham C; Nasr MA; Skarina T; Di Leo R; Kwan DH; Martin VJJ; Stogios PJ; Mahadevan R; Savchenko A; | 38696354 BIOLOGY |
| 5 | Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies | Husser MC; Pham NP; Law C; Araujo FRB; Martin VJJ; Piekny A; | 38652106 BIOLOGY |
| 6 | Genome sequencing of 15 acid-tolerant yeasts | Bagley JA; Pyne ME; Exley K; Kevvai K; Wang Q; Whiteway M; Martin VJJ; | 37747226 BIOLOGY |
| 7 | Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid | Pyne ME; Bagley JA; Narcross L; Kevvai K; Exley K; Davies M; Wang Q; Whiteway M; Martin VJJ; | 37652930 BIOLOGY |
| 8 | CRAPS: Chromosomal-Repair-Assisted Pathway Shuffling in Yeast | Dykstra CB; Pyne ME; Martin VJJ; | 37584634 BIOLOGY |
| 9 | Divergent directed evolution of a TetR-type repressor towards aromatic molecules | Nasr MA; Martin VJJ; Kwan DH; | 37377432 BIOLOGY |
| 10 | Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera) | Pyne ME; Gold ND; Martin VJJ; | 37004909 BIOLOGY |
| 11 | Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA | Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ; | 36416720 BIOLOGY |
| 12 | The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit | Bean BDM; Whiteway M; Martin VJJ; | 35708612 BIOLOGY |
| 13 | Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols | Bean BDM; Mulvihill CJ; Garge RK; Boutz DR; Rousseau O; Floyd BM; Cheney W; Gardner EC; Ellington AD; Marcotte EM; Gollihar JD; Whiteway M; Martin VJJ; | 35610225 BIOLOGY |
| 14 | A Versatile Transcription Factor Biosensor System Responsive to Multiple Aromatic and Indole Inducers | Nasr MA; Timmins LR; Martin VJJ; Kwan DH; | 35316041 CHEMBIOCHEM |
| 15 | Engineering Yeast for De Novo Synthesis of the Insect Repellent Nepetalactone | Davies ME; Tsyplenkov D; Martin VJJ; | 34748704 BIOLOGY |
| 16 | A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids. | Pyne ME, Kevvai K, Grewal PS, Narcross L, Choi B, Bourgeois L, Dueber JE, Martin VJJ | 32620756 BIOLOGY |
| 17 | Author Correction: Building a global alliance of biofoundries. | Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS | 31296848 CHEMBIOCHEM |
| 18 | An Engineered Aro1 Protein Degradation Approach for Increased cis,cis-Muconic Acid Biosynthesis in Saccharomyces cerevisiae. | Pyne ME, Narcross L, Melgar M, Kevvai K, Mookerjee S, Leite GB, Martin VJJ | 29934332 BIOLOGY |
| 19 | A Highly Characterized Synthetic Landing Pad System for Precise Multicopy Gene Integration in Yeast. | Bourgeois L, Pyne ME, Martin VJJ | 30372609 BIOLOGY |
| 20 | Microbial Factories for the Production of Benzylisoquinoline Alkaloids. | Narcross L, Fossati E, Bourgeois L, Dueber JE, Martin VJJ | 26775900 BIOLOGY |
| 21 | Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii | Zhan Y; Marchand CH; Maes A; Mauries A; Sun Y; Dhaliwal JS; Uniacke J; Arragain S; Jiang H; Gold ND; Martin VJJ; Lemaire SD; Zerges W; | 29481573 CSFG |
| 22 | Determinants of selection in yeast evolved by genome shuffling. | Biot-Pelletier D, Pinel D, Larue K, Martin VJJ | 30356826 CSFG |
| 23 | A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast. | Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ | 30474973 CSFG |
| 24 | Engineering Plant Secondary Metabolism in Microbial Systems. | Pyne ME, Narcross L, Martin VJJ | 30643013 CSFG |
| 25 | Building a global alliance of biofoundries. | Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS | 31068573 CHEMBIOCHEM |
| Title: | The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit | ||||
| Authors: | Bean BDM, Whiteway M, Martin VJJ | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/35708612/ | ||||
| DOI: | 10.1093/g3journal/jkac154 | ||||
| Publication: | G3 (Bethesda, Md.) | ||||
| Keywords: | CRISPR; Cas9; MyLO; colocalization; integration; localization; microscopy; overexpression; toolkit; yeast; | ||||
| PMID: | 35708612 | Category: | Date Added: | 2022-06-16 | |
| Dept Affiliation: |
BIOLOGY
1 Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, H4B1R6, Canada. |
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Description: |
The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful CRISPR-Cas9-based genome editing tools we constructed a Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 toolkit with three components: (i) a set of optimized S. pyogenes Cas9-guide RNA (gRNA) expression vectors with five selectable markers and the option to either pre-clone or co-transform the gRNAs; (ii) vectors for the one-step construction of integration cassettes expressing an untagged or GFP/RFP/HA-tagged gene of interest at one of three levels, supporting localization and overexpression studies; and (iii) integration cassettes containing moderately expressed GFP- or RFP-tagged compartmental markers for colocalization experiments. These components allow rapid, high efficiency genomic integrations and modifications with only transient selection for the Cas9 vector, resulting in markerless transformations. To demonstrate the ease of use, we applied our complete set of compartmental markers to co-label all target subcellular compartments with GFP and RFP. Thus, the MyLO toolkit packages CRISPR-Cas9 technology into a flexible, optimized bundle that allows the stable genomic integration of DNA with the ease of use approaching that of transforming plasmids. |
