Keyword search (4,163 papers available)

"Martin VJJ" Authored Publications:

Title Authors PubMed ID
1 Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield Narcross L; Pyne ME; Kevvai K; Siu KH; Dueber JE; Martin VJJ; 41779670
BIOLOGY
2 Tri-Functional CRISPR Screen Reveals Overexpression of em QDR2 /em and em QDR3 /em Transporters Increase Fumaric Acid Production in em Kluyveromyces marxianus /em Thornbury M; Omran RP; Kumar L; Knoops A; Abushahin R; Whiteway M; Martin VJJ; 41277095
BIOLOGY
3 Sequencing of a Dairy Isolate Unlocks em Kluyveromyces marxianus /em as a Host for Lactose Valorization Thornbury M; Knoops A; Summerby-Murray I; Dhaliwal J; Johnson S; Utomo JC; Joshi J; Narcross L; Remondetto G; Pouliot M; Whiteway M; Martin VJJ; 40629255
BIOLOGY
4 Functional and structural characterization of an IclR family transcription factor for the development of dicarboxylic acid biosensors Pham C; Nasr MA; Skarina T; Di Leo R; Kwan DH; Martin VJJ; Stogios PJ; Mahadevan R; Savchenko A; 38696354
BIOLOGY
5 Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies Husser MC; Pham NP; Law C; Araujo FRB; Martin VJJ; Piekny A; 38652106
BIOLOGY
6 Genome sequencing of 15 acid-tolerant yeasts Bagley JA; Pyne ME; Exley K; Kevvai K; Wang Q; Whiteway M; Martin VJJ; 37747226
BIOLOGY
7 Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid Pyne ME; Bagley JA; Narcross L; Kevvai K; Exley K; Davies M; Wang Q; Whiteway M; Martin VJJ; 37652930
BIOLOGY
8 CRAPS: Chromosomal-Repair-Assisted Pathway Shuffling in Yeast Dykstra CB; Pyne ME; Martin VJJ; 37584634
BIOLOGY
9 Divergent directed evolution of a TetR-type repressor towards aromatic molecules Nasr MA; Martin VJJ; Kwan DH; 37377432
BIOLOGY
10 Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera) Pyne ME; Gold ND; Martin VJJ; 37004909
BIOLOGY
11 Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ; 36416720
BIOLOGY
12 The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit Bean BDM; Whiteway M; Martin VJJ; 35708612
BIOLOGY
13 Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols Bean BDM; Mulvihill CJ; Garge RK; Boutz DR; Rousseau O; Floyd BM; Cheney W; Gardner EC; Ellington AD; Marcotte EM; Gollihar JD; Whiteway M; Martin VJJ; 35610225
BIOLOGY
14 A Versatile Transcription Factor Biosensor System Responsive to Multiple Aromatic and Indole Inducers Nasr MA; Timmins LR; Martin VJJ; Kwan DH; 35316041
CHEMBIOCHEM
15 Engineering Yeast for De Novo Synthesis of the Insect Repellent Nepetalactone Davies ME; Tsyplenkov D; Martin VJJ; 34748704
BIOLOGY
16 A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids. Pyne ME, Kevvai K, Grewal PS, Narcross L, Choi B, Bourgeois L, Dueber JE, Martin VJJ 32620756
BIOLOGY
17 Author Correction: Building a global alliance of biofoundries. Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS 31296848
CHEMBIOCHEM
18 An Engineered Aro1 Protein Degradation Approach for Increased cis,cis-Muconic Acid Biosynthesis in Saccharomyces cerevisiae. Pyne ME, Narcross L, Melgar M, Kevvai K, Mookerjee S, Leite GB, Martin VJJ 29934332
BIOLOGY
19 A Highly Characterized Synthetic Landing Pad System for Precise Multicopy Gene Integration in Yeast. Bourgeois L, Pyne ME, Martin VJJ 30372609
BIOLOGY
20 Microbial Factories for the Production of Benzylisoquinoline Alkaloids. Narcross L, Fossati E, Bourgeois L, Dueber JE, Martin VJJ 26775900
BIOLOGY
21 Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii Zhan Y; Marchand CH; Maes A; Mauries A; Sun Y; Dhaliwal JS; Uniacke J; Arragain S; Jiang H; Gold ND; Martin VJJ; Lemaire SD; Zerges W; 29481573
CSFG
22 Determinants of selection in yeast evolved by genome shuffling. Biot-Pelletier D, Pinel D, Larue K, Martin VJJ 30356826
CSFG
23 A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast. Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ 30474973
CSFG
24 Engineering Plant Secondary Metabolism in Microbial Systems. Pyne ME, Narcross L, Martin VJJ 30643013
CSFG
25 Building a global alliance of biofoundries. Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS 31068573
CHEMBIOCHEM

 

Title:A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids.
Authors:Pyne MEKevvai KGrewal PSNarcross LChoi BBourgeois LDueber JEMartin VJJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/32620756?dopt=Abstract
DOI:10.1038/s41467-020-17172-x
Publication:Nature communications
Keywords:
PMID:32620756 Category:Nat Commun Date Added:2020-07-06
Dept Affiliation: BIOLOGY
1 Department of Biology, Concordia University, Montréal, QC, Canada.
2 Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, Canada.
3 Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
4 Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
5 Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
6 Department of Biology, Concordia University, Montréal, QC, Canada. vincent.martin@concordia.ca.
7 Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, Canada. vincent.martin@concordia.ca.

Description:

A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids.

Nat Commun. 2020 Jul 03;11(1):3337

Authors: Pyne ME, Kevvai K, Grewal PS, Narcross L, Choi B, Bourgeois L, Dueber JE, Martin VJJ

Abstract

The tetrahydroisoquinoline (THIQ) moiety is a privileged substructure of many bioactive natural products and semi-synthetic analogs. Plants manufacture more than 3,000 THIQ alkaloids, including the opioids morphine and codeine. While microbial species have been engineered to synthesize a few compounds from the benzylisoquinoline alkaloid (BIA) family of THIQs, low product titers impede industrial viability and limit access to the full chemical space. Here we report a yeast THIQ platform by increasing production of the central BIA intermediate (S)-reticuline to 4.6?g?L-1, a 57,000-fold improvement over our first-generation strain. We show that gains in BIA output coincide with the formation of several substituted THIQs derived from amino acid catabolism. We use these insights to repurpose the Ehrlich pathway and synthesize an array of THIQ structures. This work provides a blueprint for building diverse alkaloid scaffolds and enables the targeted overproduction of thousands of THIQ products, including natural and semi-synthetic opioids.

PMID: 32620756 [PubMed - as supplied by publisher]





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