Keyword search (4,163 papers available)

"synthesis" Keyword-tagged Publications:

Title Authors PubMed ID
1 From tissue to sound: A new paradigm for medical sonic interaction design Matinfar S; Dehghani S; Salehi M; Sommersperger M; Navab N; Faridpooya K; Fairhurst M; Navab N; 40222195
CONCORDIA
2 Hyperbranched Polyethylene Ionomers Containing Quaternary Ammonium Ions and Their Functionalization of Nanomaterials Ye Z; Rahmatinejad J; Raisi B; Dai P; 40214570
ENCS
3 Searching and reporting in Campbell Collaboration systematic reviews: A systematic assessment of current methods Young S; MacDonald H; Louden D; Ellis UM; Premji Z; Rogers M; Bethel A; Pickup D; 39176233
CONCORDIA
4 Pitavastatin Calcium Confers Fungicidal Properties to Fluconazole by Inhibiting Ubiquinone Biosynthesis and Generating Reactive Oxygen Species Li W; Feng Y; Feng Z; Wang L; Whiteway M; Lu H; Jiang Y; 38929106
BIOLOGY
5 Advancements in Hybrid Cellulose-Based Films: Innovations and Applications in 2D Nano-Delivery Systems Ramezani G; Stiharu I; van de Ven TGM; Nerguizian V; 38667550
ENCS
6 What is Learned Determines How Pavlovian Conditioned Fear is Consolidated in the Brain Leake J; Leidl DM; Lay BPP; Fam JP; Giles MC; Qureshi OA; Westbrook RF; Holmes NM; 37963767
CSBN
7 Engineering the Surface Chemistry and Morphology of Polymeric Carbon Nitrides Towards Greener Heterogeneous Catalysts for Biodiesel Synthesis de Medeiros TV; Macina A; Bicalho HA; Naccache R; 37058095
CHEMBIOCHEM
8 Oligonucleotides Containing C5-Propynyl Modified Arabinonucleic Acids: Synthesis, Biophysical and Antisense Properties Pontarelli A; Wilds CJ; 36857293
CHEMBIOCHEM
9 A "biphasic glycosyltransferase high-throughput screen" identifies novel anthraquinone glycosides in the diversification of phenolic natural products Mohideen FI; Kwan DH; 36682498
CHEMBIOCHEM
10 Photoactivation and conformational gating for manganese binding and oxidation in bacterial reaction centers Samaei A; Deshmukh SS; Protheroe C; Nyéki S; Tremblay-Ethier RA; Kálmán L; 36216075
PHYSICS
11 Simple, Economical Methods for the Culture of Green Algae for Energy Harvesting from Photosynthesis in a Microfluidic Environment Kuruvinashetti K; Rahimi S; Pakkiriswami S; Packirisamy M; 34898042
ENCS
12 Binary Cu2-xS Templates Direct the Formation of Quaternary Cu2ZnSnS4 (Kesterite, Wurtzite) Nanocrystals Yarur Villanueva F; Green PB; Qiu C; Ullah SR; Buenviaje K; Howe JY; Majewski MB; Wilson MWB; 34705409
CNSR
13 Sustainable chemical processing of flowing wastewater through microwave energy Siddique F; Mirzaei A; Gonzalez-Cortes S; Slocombe D; Al-Megren HA; Xiao T; Rafiq MA; Edwards PP; 34474383
PHYSICS
14 Tuning the redox potential of the primary electron donor in bacterial reaction centers by manganese binding and light-induced structural changes. Deshmukh SS, Kálmán L 32777306
PHYSICS
15 Biosynthesis of Alkylcitric Acids in Aspergillus niger Involves Both Co-localized and Unlinked Genes. Palys S, Pham TTM, Tsang A 32695080
CSFG
16 Bound detergent molecules in bacterial reaction centers facilitate detection of tetryl explosive. Modafferi D, Zazubovich V, Kálmán L 32632533
PHYSICS
17 Transcriptomic analysis suggests the inhibition of DNA damage repair in green alga Raphidocelis subcapitata exposed to roxithromycin. Guo J, Bai Y, Chen Z, Mo J, Li Q, Sun H, Zhang Q 32505758
CHEMISTRY
18 Surface Response Based Modeling of Liposome Characteristics in a Periodic Disturbance Mixer. López RR, Ocampo I, Sánchez LM, Alazzam A, Bergeron KF, Camacho-León S, Mounier C, Stiharu I, Nerguizian V 32106424
ENCS
19 Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance and total tract digestibility of heifers fed a high forage diet. Ran T, Saleem AM, Shen Y, Ribeiro GO, Beauchemin KA, Tsang A, Yang W, McAllister TA 31251799
CSFG
20 Microbial Factories for the Production of Benzylisoquinoline Alkaloids. Narcross L, Fossati E, Bourgeois L, Dueber JE, Martin VJJ 26775900
BIOLOGY
21 Characterization of two novel antimicrobial peptides from the cuticular extracts of the ant Trichomyrmex criniceps (Mayr), (Hymenoptera: Formicidae). Bhagavathula N, Meedidoddi V, Bourque S, Vimaladevi R, Kesavakurup S, Selvadurai D, Shrivastava S, Krishnappa C 28346717
PERFORM
22 Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme. Kwan DH 28935105
CSFG
23 A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast. Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ 30474973
CSFG

 

Title:Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme.
Authors:Kwan DH
Link:https://www.ncbi.nlm.nih.gov/pubmed/28935105?dopt=Abstract
DOI:10.1016/bs.mie.2017.06.002
Publication:Methods in enzymology
Keywords:Blood group antigenDirected evolutionEnzymatic oligosaccharide synthesisEnzyme engineeringGlycosidaseHigh-throughput assaysSemirational design
PMID:28935105 Category:Methods Enzymol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Centre for Applied Synthetic Biology, Centre for Structural and Functional Genomics, Concordia University, Montréal, Québec, Canada. Electronic address: david.kwan@concordia.ca.

Description:

Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme.

Methods Enzymol. 2017;597:25-53

Authors: Kwan DH

Abstract

Directed evolution is an incredibly powerful strategy for engineering enzyme function. Applying this approach to glycosidases offers enormous potential for the development of highly specialized tools in chemical glycobiology. Performing enzyme directed evolution requires the generation, by random mutagenesis, of mutant libraries from which large numbers of variant enzymes must be screened in high-throughput assays. A structure-guided "semirational" method for library creation allows researchers to target specific amino acid positions for mutagenesis, concentrating mutations where they might be most effective in order to produce mutant libraries of a manageable size, minimizing screening effort while maximizing the chances of finding improved mutants. Well-designed assays, which may use specially prepared substrates, enable efficient screening of these mutant libraries. This chapter will detail general methods in the structure-guided directed evolution of glycosidases, which have previously been employed in engineering a blood group antigen-cleaving enzyme.

PMID: 28935105 [PubMed - indexed for MEDLINE]





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