PERFORM Publications

Keyword search (4,163 papers available)

Title		Authors	PubMed ID
1	From tissue to sound: A new paradigm for medical sonic interaction design	Matinfar S; Dehghani S; Salehi M; Sommersperger M; Navab N; Faridpooya K; Fairhurst M; Navab N;	40222195 CONCORDIA
2	Hyperbranched Polyethylene Ionomers Containing Quaternary Ammonium Ions and Their Functionalization of Nanomaterials	Ye Z; Rahmatinejad J; Raisi B; Dai P;	40214570 ENCS
3	Searching and reporting in Campbell Collaboration systematic reviews: A systematic assessment of current methods	Young S; MacDonald H; Louden D; Ellis UM; Premji Z; Rogers M; Bethel A; Pickup D;	39176233 CONCORDIA
4	Pitavastatin Calcium Confers Fungicidal Properties to Fluconazole by Inhibiting Ubiquinone Biosynthesis and Generating Reactive Oxygen Species	Li W; Feng Y; Feng Z; Wang L; Whiteway M; Lu H; Jiang Y;	38929106 BIOLOGY
5	Advancements in Hybrid Cellulose-Based Films: Innovations and Applications in 2D Nano-Delivery Systems	Ramezani G; Stiharu I; van de Ven TGM; Nerguizian V;	38667550 ENCS
6	What is Learned Determines How Pavlovian Conditioned Fear is Consolidated in the Brain	Leake J; Leidl DM; Lay BPP; Fam JP; Giles MC; Qureshi OA; Westbrook RF; Holmes NM;	37963767 CSBN
7	Engineering the Surface Chemistry and Morphology of Polymeric Carbon Nitrides Towards Greener Heterogeneous Catalysts for Biodiesel Synthesis	de Medeiros TV; Macina A; Bicalho HA; Naccache R;	37058095 CHEMBIOCHEM
8	Oligonucleotides Containing C5-Propynyl Modified Arabinonucleic Acids: Synthesis, Biophysical and Antisense Properties	Pontarelli A; Wilds CJ;	36857293 CHEMBIOCHEM
9	A "biphasic glycosyltransferase high-throughput screen" identifies novel anthraquinone glycosides in the diversification of phenolic natural products	Mohideen FI; Kwan DH;	36682498 CHEMBIOCHEM
10	Photoactivation and conformational gating for manganese binding and oxidation in bacterial reaction centers	Samaei A; Deshmukh SS; Protheroe C; Nyéki S; Tremblay-Ethier RA; Kálmán L;	36216075 PHYSICS
11	Simple, Economical Methods for the Culture of Green Algae for Energy Harvesting from Photosynthesis in a Microfluidic Environment	Kuruvinashetti K; Rahimi S; Pakkiriswami S; Packirisamy M;	34898042 ENCS
12	Binary Cu2-xS Templates Direct the Formation of Quaternary Cu2ZnSnS4 (Kesterite, Wurtzite) Nanocrystals	Yarur Villanueva F; Green PB; Qiu C; Ullah SR; Buenviaje K; Howe JY; Majewski MB; Wilson MWB;	34705409 CNSR
13	Sustainable chemical processing of flowing wastewater through microwave energy	Siddique F; Mirzaei A; Gonzalez-Cortes S; Slocombe D; Al-Megren HA; Xiao T; Rafiq MA; Edwards PP;	34474383 PHYSICS
14	Tuning the redox potential of the primary electron donor in bacterial reaction centers by manganese binding and light-induced structural changes.	Deshmukh SS, Kálmán L	32777306 PHYSICS
15	Biosynthesis of Alkylcitric Acids in Aspergillus niger Involves Both Co-localized and Unlinked Genes.	Palys S, Pham TTM, Tsang A	32695080 CSFG
16	Bound detergent molecules in bacterial reaction centers facilitate detection of tetryl explosive.	Modafferi D, Zazubovich V, Kálmán L	32632533 PHYSICS
17	Transcriptomic analysis suggests the inhibition of DNA damage repair in green alga Raphidocelis subcapitata exposed to roxithromycin.	Guo J, Bai Y, Chen Z, Mo J, Li Q, Sun H, Zhang Q	32505758 CHEMISTRY
18	Surface Response Based Modeling of Liposome Characteristics in a Periodic Disturbance Mixer.	López RR, Ocampo I, Sánchez LM, Alazzam A, Bergeron KF, Camacho-León S, Mounier C, Stiharu I, Nerguizian V	32106424 ENCS
19	Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance and total tract digestibility of heifers fed a high forage diet.	Ran T, Saleem AM, Shen Y, Ribeiro GO, Beauchemin KA, Tsang A, Yang W, McAllister TA	31251799 CSFG
20	Microbial Factories for the Production of Benzylisoquinoline Alkaloids.	Narcross L, Fossati E, Bourgeois L, Dueber JE, Martin VJJ	26775900 BIOLOGY
21	Characterization of two novel antimicrobial peptides from the cuticular extracts of the ant Trichomyrmex criniceps (Mayr), (Hymenoptera: Formicidae).	Bhagavathula N, Meedidoddi V, Bourque S, Vimaladevi R, Kesavakurup S, Selvadurai D, Shrivastava S, Krishnappa C	28346717 PERFORM
22	Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme.	Kwan DH	28935105 CSFG
23	A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.	Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ	30474973 CSFG

Title:	A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.
Authors:	Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ
Link:	https://www.ncbi.nlm.nih.gov/pubmed/30474973?dopt=Abstract
DOI:	10.1021/acssynbio.8b00470
Publication:	ACS synthetic biology
Keywords:	cytochrome P450 monooxygenase; cytochrome P450 reductase; diterpenoid biosynthesis; steviol glucosides;
PMID:	30474973	Category:	ACS Synth Biol	Date Added:	2019-06-07
Dept Affiliation:	CSFG 1 Centre for Applied Synthetic Biology , Concordia University , Montréal , Québec H4B 1R6 , Canada. 2 Plant Biochemistry Laboratory, Department of Plant and Environmental Science , University of Copenhagen , DK-1871 Frederiksberg C , Denmark. 3 Center for Synthetic Biology , University of Copenhagen , DK-1871 Frederiksberg C , Denmark. 4 Centre for Structural and Functional Genomics , Concordia University , Montréal , Québec H4B 1R6 , Canada. 5 Evolva , Lersø Parkallé 42-44 , DK-2100 Copenhagen Ø , Denmark. 6 Department of Biology, Centre for Structural and Functional Genomic , Concordia University , Montréal , Québec H4B 1R6 , Canada.

Description:

A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.

ACS Synth Biol. 2018 Dec 21;7(12):2918-2929

Authors: Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ

Abstract

Biosynthesis of steviol glycosides in planta proceeds via two cytochrome P450 enzymes (CYPs): kaurene oxidase (KO) and kaurenoic acid hydroxylase (KAH). KO and KAH function in succession with the support of a NADPH-dependent cytochrome P450 reductase (CPR) to convert kaurene to steviol. This work describes a platform for recombinant production of steviol glucosides (SGs) in Saccharomyces cerevisiae, demonstrating the full reconstituted pathway from the simple sugar glucose to the SG precursor steviol. With a focus on optimization of the KO-KAH activities, combinations of functional homologues were tested in batch growth. Among the CYPs, novel KO75 (CYP701) and novel KAH82 (CYP72) outperformed their respective functional homologues from Stevia rebaudiana, SrKO (CYP701A5) and SrKAH (CYP81), in assays where substrate was supplemented to culture broth. With kaurene produced from glucose in the cell, SrCPR1 from S. rebaudiana supported highest turnover for KO-KAH combinations, besting two other CPRs isolated from S. rebaudiana, the Arabidopsis thaliana ATR2, and a new class I CPR12. Some coexpressions of ATR2 with a second CPR were found to diminish KAH activity, showing that coexpression of CPRs can lead to competition for CYPs with possibly adverse effects on catalysis.

PMID: 30474973 [PubMed - in process]

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