PERFORM Publications

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Title		Authors	PubMed ID
1	Expansion of Auxiliary Activity Family 5 sequence space via biochemical characterization of six new copper radical oxidases	Fong JK; Mathieu Y; Vo MT; Bellemare A; Tsang A; Brumer H;	38953370 CSFG
2	Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics	Gruninger RJ; Tsang A; McAllister TA;	37149538 CSFG
3	Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls	Derba-Maceluch M; Mitra M; Hedenström M; Liu X; Gandla ML; Barbut FR; Abreu IN; Donev EN; Urbancsok J; Moritz T; Jönsson LJ; Tsang A; Powlowski J; Master ER; Mellerowicz EJ;	36600379 CSFG
4	On the Origin of Sugar Handedness: Facts, Hypotheses and Missing Links-A Review	Martínez RF; Cuccia LA; Viedma C; Cintas P;	35796896 CHEMBIOCHEM
5	Editorial: The Effect of Carbohydrate Restriction on Cancer and Metabolic Syndrome	Elisia I; Santosa S; Popovich DG; Krystal G;	35237644 HKAP
6	Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases	Dilokpimol A; Verkerk B; Li X; Bellemare A; Lavallee M; Frommhagen M; Nørmølle Underlin E; Kabel MA; Powlowski J; Tsang A; de Vries RP;	35187647 CSFG
7	Resources and Methods for Engineering "Designer" Glycan-Binding Proteins.	Warkentin R, Kwan DH	33450899 CHEMBIOCHEM
8	Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila.	Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A	24881579 BIOLOGY
9	Evaluation of secretome of highly efficient lignocellulolytic Penicillium sp. Dal 5 isolated from rhizosphere of conifers.	Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS	27341464 CSFG
10	Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics.	Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA	28417376 CSFG
11	Discovery and characterization of family 39 glycoside hydrolases from rumen anaerobic fungi with polyspecific activity on rare arabinosyl substrates.	Jones DR, Uddin MS, Gruninger RJ, Pham TTM, Thomas D, Boraston AB, Briggs J, Pluvinage B, McAllister TA, Forster RJ, Tsang A, Selinger LB, Abbott DW	28588026 CSFG
12	Identification of novel enzymes to enhance the ruminal digestion of barley straw	Badhan A; Ribeiro GO; Jones DR; Wang Y; Abbott DW; Di Falco M; Tsang A; McAllister TA;	29621684 CSFG
13	Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.	Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T	29803771 CSFG
14	Application of Transcriptomics to Compare the Carbohydrate Active Enzymes That Are Expressed by Diverse Genera of Anaerobic Fungi to Degrade Plant Cell Wall Carbohydrates.	Gruninger RJ, Nguyen TTM, Reid ID, Yanke JL, Wang P, Abbott DW, Tsang A, McAllister T	30061875 CSFG

Title:	Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.
Authors:	Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T
Link:	https://www.ncbi.nlm.nih.gov/pubmed/29803771?dopt=Abstract
DOI:	10.1016/j.nbt.2018.05.003
Publication:	New biotechnology
Keywords:	Aerobic fungi; Barley straw; Blue native PAGE; Carbohydrate active enzymes; Glycosyl hydrolase; Multi-enzyme complex;
PMID:	29803771	Category:	N Biotechnol	Date Added:	2019-06-07
Dept Affiliation:	CSFG 1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada. 2 Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, 330096, China. 3 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, H4B 1R6, Canada. 4 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada. Electronic address: tim.mcallister@agr.gc.ca.

Description:

Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.

N Biotechnol. 2018 Nov 25;46:1-6

Authors: Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

Abstract

In the present study, we have characterized high molecular weight multi-enzyme complexes in two commercial enzymes produced by Trichoderma reesei (Spezyme CP) and Penicillium funiculosum (Accellerase XC). We successfully identified 146-1000?kDa complexes using Blue native polyacrylamide gel electrophoresis (BN-PAGE) to fractionate the protein profile in both preparations. Identified complexes dissociated into lower molecular weight constituents when loaded on SDS PAGE. Unfolding of the secondary structure of multi-enzyme complexes with trimethylamine (pH >10) suggested that they were not a result of unspecific protein aggregation. Cellulase (CMCase) profiles of extracts of BN-PAGE fractionated protein bands confirmed cellulase activity within the multi-enzyme complexes. A microassay was used to identify protein bands that promoted high levels of glucose release from barley straw. Those with high saccharification yield were subjected to LC-MS analysis to identify the principal enzymatic activities responsible. The results suggest that secretion of proteins by aerobic fungi leads to the formation of high molecular weight multi-enzyme complexes that display activity against carboxymethyl cellulose and barley straw.

PMID: 29803771 [PubMed - indexed for MEDLINE]

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