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PubMed Publications| Keyword search (4,163 papers available) |  |
"Biomass" Keyword-tagged Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | eDNA Provides Accurate Population Abundance Estimates With Bioenergetics and Particle Mass-Balance Modelling | Beaulieu J; Yates MC; Fraser DJ; Cristescu ME; Derry AM; | 41913704 BIOLOGY |
| 2 | Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification | Raheja Y; Singh V; Gaur VK; Sharma G; Tsang A; Chadha BS; | 40622460 GENOMICS |
| 3 | Production and characterization of magnetic Biochar derived from pyrolysis of waste areca nut husk for removal of methylene blue dye from wastewater | Chistie SM; Naik SU; Rajendra P; Apeksha None; Mishra RK; Albasher G; Chinnam S; Jeppu GP; Arif Z; Hameed J; | 40603323 ENCS |
| 4 | Evaluating Sustainable Practices for Managing Residue Derived from Wheat Straw | Shanmugam H; Raghavan V; Rajagopal R; Goyette B; Lyu L; Zhou S; An C; | 38927790 ENCS |
| 5 | Diverse Applications of Biomass-Derived 5-Hydroxymethylfurfural and Derivatives as Renewable Starting Materials | Chacón-Huete F; Messina C; Cigana B; Forgione P; | 35652539 CHEMBIOCHEM |
| 6 | Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases | Dilokpimol A; Verkerk B; Li X; Bellemare A; Lavallee M; Frommhagen M; Nørmølle Underlin E; Kabel MA; Powlowski J; Tsang A; de Vries RP; | 35187647 CSFG |
| 7 | Species compositions mediate biomass conservation: the case of lake fish communities | Arranz I; Fournier B; Lester NP; Shuter BJ; Peres-Neto PR; | 34905222 BIOLOGY |
| 8 | Use of biomass-derived adsorbents for the removal of petroleum pollutants from water: a mini-review | Vahabisani A; An C; | 34804763 ENCS |
| 9 | The relationship between eDNA particle concentration and organism abundance in nature is strengthened by allometric scaling. | Yates MC, Glaser D, Post J, Cristescu ME, Fraser DJ, Derry AM | 32638451 CONCORDIA |
| 10 | Penicillium subrubescens adapts its enzyme production to the composition of plant biomass. | Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP | 32408196 CSFG |
| 11 | Evidence for ligninolytic activity of the ascomycete fungus Podospora anserina. | van Erven G, Kleijn AF, Patyshakuliyeva A, Di Falco M, Tsang A, de Vries RP, van Berkel WJH, Kabel MA | 32322305 CSFG |
| 12 | Enzymes of early-diverging, zoosporic fungi. | Lange L, Barrett K, Pilgaard B, Gleason F, Tsang A | 31309267 CSFG |
| 13 | Pilot-scale application of a single-stage hybrid airlift BioCAST bioreactor for treatment of ammonium from nitrite-limited wastewater by a partial nitrification/anammox process. | Saborimanesh N, Walsh D, Yerushalmi L, Arriagada EC, Mulligan CN | 31267396 BIOLOGY |
| 14 | Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila. | Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A | 24881579 BIOLOGY |
| 15 | Closely related fungi employ diverse enzymatic strategies to degrade plant biomass. | Benoit I, Culleton H, Zhou M, DiFalco M, Aguilar-Osorio G, Battaglia E, Bouzid O, Brouwer CPJM, El-Bushari HBO, Coutinho PM, Gruben BS, Hildén KS, Houbraken J, Barboza LAJ, Levasseur A, Majoor E, Mäkelä MR, Narang HM, Trejo-Aguilar B, van den Brink J, vanKuyk PA, Wiebenga A, McKie V, McCleary B, Tsang A, Henrissat B, de Vries RP | 26236396 CSFG |
| 16 | Expression-based clustering of CAZyme-encoding genes of Aspergillus niger. | Gruben BS, Mäkelä MR, Kowalczyk JE, Zhou M, Benoit-Gelber I, De Vries RP | 29169319 CSFG |
| 17 | Genomic and exoproteomic diversity in plant biomass degradation approaches among Aspergilli | Mäkelä MR; DiFalco M; McDonnell E; Nguyen TTM; Wiebenga A; Hildén K; Peng M; Grigoriev IV; Tsang A; de Vries RP; | 30487660 CSFG |
| 18 | The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum. | Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP | 30797054 CSFG |
| Title: | Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification | ||||
| Authors: | Raheja Y, Singh V, Gaur VK, Sharma G, Tsang A, Chadha BS | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/40622460/ | ||||
| DOI: | 10.1007/s11274-025-04473-w | ||||
| Publication: | World journal of microbiology & biotechnology | ||||
| Keywords: | Pichia pastoris; Rasamsonia Emersonii; Hydrolysis; Lignocellulosic biomass; Recombinant cellobiohydrolases; Tailored enzyme cocktail; | ||||
| PMID: | 40622460 | Category: | Date Added: | 2025-07-07 | |
| Dept Affiliation: |
GENOMICS
1 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. yashikaraheja27@gmail.com. 2 School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919, Republic of Korea. yashikaraheja27@gmail.com. 3 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. 4 School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919, Republic of Korea. 5 Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, India. 6 Department of Microbiology, DAV university Jalandhar, Jalandhar, Punjab, 144012, India. 7 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada. |
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Description: |
GH7 cellobiohydrolases (CBH1s) are essential for depolymerizing crystalline cellulose, yet the hypercellulolytic thermophile Rasamsonia emersonii secretes them only in low amounts, leaving a gap in its native enzyme cocktail. To see whether a cognate CBH1 could fill this gap and how it stacks up against the industrial workhorse strain Trichoderma reesei Cel7A, we codon optimized the R. emersonii gene (Rem_GH7CBHI), expressed it in Pichia pastoris and purified the recombinant enzyme for structural and functional analysis. The 57 kDa protein retains the canonical GH7 ßsandwich tunnel, but an AlaforTyr substitution leaves the channel more open than that of T. reesei, potentially easing substrate entry. Consistent with this architecture, Rem_GH7CBHI binds cellotriose tightly and exhibits a low K? of 0.25 mM. Biochemical characterization revealed the optimal activity at pH 5.0, 60 °C and retaining about 60% activity after 1 h at 70 °C. Adding Rem_GH7CBHI together with an endogenous AA9 LPMO to the native R. emersonii secretome (M36) boosted saccharification of steamacidpretreated rice straw to levels comparable with commercial Cellic CTec3 at the same total protein loading. These results position Rem_GH7CBHI as a thermostable, highaffinity alternative to T. reesei Cel7A and a costeffective addition to tailored enzyme cocktails for highsolids lignocellulose biorefineries. |
