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"Nat Commun" Category Publications:

Title Authors PubMed ID
1 Auditory information enhances post-sensory visual evidence during rapid multisensory decision-making. Franzen L; Delis I; De Sousa G; Kayser C; Philiastides MG; 33116148
CONCORDIA
2 Dissociable mesolimbic dopamine circuits control responding triggered by alcohol-predictive discrete cues and contexts. Valyear MD, Glovaci I, Zaari A, Lahlou S, Trujillo-Pisanty I, Andrew Chapman C, Chaudhri N 32724058
PSYCHOLOGY
3 A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids. Pyne ME, Kevvai K, Grewal PS, Narcross L, Choi B, Bourgeois L, Dueber JE, Martin VJJ 32620756
BIOLOGY
4 Evolving perspectives on the sources of the frequency-following response. Coffey EBJ, Nicol T, White-Schwoch T, Chandrasekaran B, Krizman J, Skoe E, Zatorre RJ, Kraus N 31695046
PSYCHOLOGY
5 Bacterial variability in the mammalian gut captured by a single-cell synthetic oscillator. Riglar DT, Richmond DL, Potvin-Trottier L, Verdegaal AA, Naydich AD, Bakshi S, Leoncini E, Lyon LG, Paulsson J, Silver PA 31604953
BIOLOGY
6 Non-invasive in vivo hyperspectral imaging of the retina for potential biomarker use in Alzheimer's disease. Hadoux X, Hui F, Lim JKH, Masters CL, Pébay A, Chevalier S, Ha J, Loi S, Fowler CJ, Rowe C, Villemagne VL, Taylor EN, Fluke C, Soucy JP, Lesage F, Sylvestre JP, Rosa-Neto P, Mathotaarachchi S, Gauthier S, Nasreddine ZS, Arbour JD, Rhéaume MA, Beaulieu S, Dirani M, Nguyen CTO, Bui BV, Williamson R, Crowston JG, van Wijngaarden P 31530809
PERFORM
7 Author Correction: Building a global alliance of biofoundries. Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS 31296848
CHEMBIOCHEM
8 Neural correlates of two different types of extinction learning in the amygdala central nucleus. Iordanova MD, Deroche ML, Esber GR, Schoenbaum G 27531638
CSBN
9 The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation. McNally EK, Brett CL 30560896
BIOLOGY
10 A platform for glycoengineering a polyvalent pneumococcal bioconjugate vaccine using E. coli as a host. Harding CM, Nasr MA, Scott NE, Goyette-Desjardins G, Nothaft H, Mayer AE, Chavez SM, Huynh JP, Kinsella RL, Szymanski CM, Stallings CL, Segura M, Feldman MF 30792408
BIOLOGY
11 Reconstitution of a 10-gene pathway for synthesis of the plant alkaloid dihydrosanguinarine in Saccharomyces cerevisiae. Fossati E, Ekins A, Narcross L, Zhu Y, Falgueyret JP, Beaudoin GA, Facchini PJ, Martin VJ 24513861
BIOLOGY
12 Giant electron-hole transport asymmetry in ultra-short quantum transistors. McRae AC, Tayari V, Porter JM, Champagne AR 28561024
PHYSICS
13 Building a global alliance of biofoundries. Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS 31068573
CHEMBIOCHEM

 

Title:A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids.
Authors:Pyne MEKevvai KGrewal PSNarcross LChoi BBourgeois LDueber JEMartin VJJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/32620756?dopt=Abstract
DOI:10.1038/s41467-020-17172-x
Publication:Nature communications
Keywords:
PMID:32620756 Category:Nat Commun Date Added:2020-07-06
Dept Affiliation: BIOLOGY
1 Department of Biology, Concordia University, Montréal, QC, Canada.
2 Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, Canada.
3 Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
4 Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
5 Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
6 Department of Biology, Concordia University, Montréal, QC, Canada. vincent.martin@concordia.ca.
7 Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, Canada. vincent.martin@concordia.ca.

Description:

A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids.

Nat Commun. 2020 Jul 03;11(1):3337

Authors: Pyne ME, Kevvai K, Grewal PS, Narcross L, Choi B, Bourgeois L, Dueber JE, Martin VJJ

Abstract

The tetrahydroisoquinoline (THIQ) moiety is a privileged substructure of many bioactive natural products and semi-synthetic analogs. Plants manufacture more than 3,000 THIQ alkaloids, including the opioids morphine and codeine. While microbial species have been engineered to synthesize a few compounds from the benzylisoquinoline alkaloid (BIA) family of THIQs, low product titers impede industrial viability and limit access to the full chemical space. Here we report a yeast THIQ platform by increasing production of the central BIA intermediate (S)-reticuline to 4.6?g?L-1, a 57,000-fold improvement over our first-generation strain. We show that gains in BIA output coincide with the formation of several substituted THIQs derived from amino acid catabolism. We use these insights to repurpose the Ehrlich pathway and synthesize an array of THIQ structures. This work provides a blueprint for building diverse alkaloid scaffolds and enables the targeted overproduction of thousands of THIQ products, including natural and semi-synthetic opioids.

PMID: 32620756 [PubMed - as supplied by publisher]





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