PERFORM Publications

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Title		Authors	PubMed ID
1	Deformable detection transformers for domain adaptable ultrasound localization microscopy with robustness to point spread function variations	Gharamaleki SK; Helfield B; Rivaz H;	40640235 PHYSICS
2	Measuring prion propagation in single bacteria elucidates a mechanism of loss	Jager K; Orozco-Hidalgo MT; Springstein BL; Joly-Smith E; Papazotos F; McDonough E; Fleming E; McCallum G; Yuan AH; Hilfinger A; Hochschild A; Potvin-Trottier L;	37738299 PHYSICS
3	Impact of Pollutant Ozone on the Biophysical Properties of Tear Film Lipid Layer Model Membranes	Keramatnejad M; DeWolf C;	36837668 CHEMBIOCHEM
4	A biophysical study of tear film lipid layer model membranes	Keramatnejad M; DeWolf C;	36535341 CNSR
5	Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA	Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ;	36416720 BIOLOGY
6	Microfluidics for long-term single-cell time-lapse microscopy: Advances and applications	Allard P; Papazotos F; Potvin-Trottier L;	36312536 BIOLOGY
7	A Deep Learning Approach to Capture the Essence of Candida albicans Morphologies	Bettauer V; Costa ACBP; Omran RP; Massahi S; Kirbizakis E; Simpson S; Dumeaux V; Law C; Whiteway M; Hallett MT;	35972285 BIOLOGY
8	Gold Nano-Bio-Interaction to Modulate Mechanobiological Responses for Cancer Therapy Applications	Sohrabi Kashani A; Larocque K; Piekny A; Packirisamy M;	35839330 BIOLOGY
9	The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit	Bean BDM; Whiteway M; Martin VJJ;	35708612 BIOLOGY
10	Estrogen receptors observed at extranuclear neuronal sites and in glia in the nucleus accumbens core and shell of the female rat: Evidence for localization to catecholaminergic and GABAergic neurons	Almey A; Milner TA; Brake WG;	35397175 CSBN
11	Comparing microscopy and DNA metabarcoding techniques for identifying cyanobacteria assemblages across hundreds of lakes	MacKeigan PW; Garner RE; Monchamp MÈ; Walsh DA; Onana VE; Kraemer SA; Pick FR; Beisner BE; Agbeti MD; da Costa NB; Shapiro BJ; Gregory-Eaves I;	35287928 BIOLOGY
12	A comparative analysis of deep learning architectures on high variation malaria parasite classification dataset.	Rahman A, Zunair H, Reme TR, Rahman MS, Mahdy MRC	33465520 ENCS
13	Estrogen receptor α and G-protein coupled estrogen receptor 1 are localized to GABAergic neurons in the dorsal striatum.	Almey A, Milner TA, Brake WG	27080432 PSYCHOLOGY
14	Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.	Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL	30317518 BIOLOGY
15	Comparative morphology and phagocytic capacity of primary human adult microglia with time-lapse imaging.	Levtova N, Healy LM, Gonczi CMC, Stopnicki B, Blain M, Kennedy TE, Moore CS, Antel JP, Darlington PJ	28606377 PERFORM

Title:	Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.
Authors:	Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL
Link:	https://www.ncbi.nlm.nih.gov/pubmed/30317518?dopt=Abstract
DOI:	10.1007/978-1-4939-8760-3_24
Publication:	Methods in molecular biology (Clifton, N.J.)
Keywords:	Cryogenic electron microscopy (cryoEM); Hemifusion; Lipid bilayer merger; Lysosome; Membrane fusion; SNARE; Tomography; Transmission electron microscopy (TEM); Vacuole;
PMID:	30317518	Category:	Methods Mol Biol	Date Added:	2019-06-07
Dept Affiliation:	BIOLOGY 1 Department of Biology, Concordia University, Montréal, QC, Canada. 2 Montreal Neurological Hospital and Institute, McGill University, Montréal, QC, Canada. 3 Facility for Electron Microscopy Research, Department of Anatomy and Cell Biology, McGill University, Montréal, QC, Canada. 4 Department of Biology, Concordia University, Montréal, QC, Canada. christopher.brett@concordia.ca.

Description:

Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.

Methods Mol Biol. 2019;1860:361-377

Authors: Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL

Abstract

SNARE-mediated membrane fusion is required for membrane trafficking as well as organelle biogenesis and homeostasis. The membrane fusion reaction involves sequential formation of hemifusion intermediates, whereby lipid monolayers partially mix on route to complete bilayer merger. Studies of the Saccharomyces cerevisiae lysosomal vacuole have revealed many of the fundamental mechanisms that drive the membrane fusion process, as well as features unique to organelle fusion. However, until recently, it has not been amenable to electron microscopy methods that have been invaluable for studying hemifusion in other model systems. Herein, we describe a method to visualize hemifusion intermediates during homotypic vacuole membrane fusion in vitro by transmission electron microscopy (TEM), electron tomography, and cryogenic electron microscopy (cryoEM). This method facilitates acquisition of invaluable ultrastructural data needed to comprehensively understand how fusogenic lipids and proteins contribute to SNARE-mediated membrane fusion-by-hemifusion and the unique features of organelle versus small-vesicle fusion.

PMID: 30317518 [PubMed - indexed for MEDLINE]

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