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PubMed Publications| Keyword search (4,163 papers available) |  |
"enzymes" Keyword-tagged Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Functional screening pipeline to uncover laccase-like multicopper oxidase enzymes that transform industrial lignins | Sharan AA; Bellemare A; DiFalco M; Tsang A; Vuong TV; Edwards EA; Master ER; | 38000639 CSFG |
| 2 | Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization in Aspergillus niger | Arentshorst M; Falco MD; Moisan MC; Reid ID; Spaapen TOM; van Dam J; Demirci E; Powlowski J; Punt PJ; Tsang A; Ram AFJ; | 37744122 CSFG |
| 3 | Evidence for ligninolytic activity of the ascomycete fungus Podospora anserina. | van Erven G, Kleijn AF, Patyshakuliyeva A, Di Falco M, Tsang A, de Vries RP, van Berkel WJH, Kabel MA | 32322305 CSFG |
| 4 | Effect and ameliorative mechanisms of polyoxometalates on the denitrification under sulfonamide antibiotics stress. | Guo H, Chen Z, Lu C, Guo J, Li H, Song Y, Han Y, Hou Y | 32145698 ENCS |
| 5 | Enzymes of early-diverging, zoosporic fungi. | Lange L, Barrett K, Pilgaard B, Gleason F, Tsang A | 31309267 CSFG |
| 6 | Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila. | Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A | 24881579 BIOLOGY |
| 7 | Identification of novel enzymes to enhance the ruminal digestion of barley straw | Badhan A; Ribeiro GO; Jones DR; Wang Y; Abbott DW; Di Falco M; Tsang A; McAllister TA; | 29621684 CSFG |
| 8 | Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi. | Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T | 29803771 CSFG |
| 9 | New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw. | Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA | 30053012 CSFG |
| 10 | Application of Transcriptomics to Compare the Carbohydrate Active Enzymes That Are Expressed by Diverse Genera of Anaerobic Fungi to Degrade Plant Cell Wall Carbohydrates. | Gruninger RJ, Nguyen TTM, Reid ID, Yanke JL, Wang P, Abbott DW, Tsang A, McAllister T | 30061875 CSFG |
| 11 | The obligate alkalophilic soda-lake fungus Sodiomyces alkalinus has shifted to a protein diet. | Grum-Grzhimaylo AA, Falkoski DL, van den Heuvel J, Valero-Jiménez CA, Min B, Choi IG, Lipzen A, Daum CG, Aanen DK, Tsang A, Henrissat B, Bilanenko EN, de Vries RP, van Kan JAL, Grigoriev IV, Debets AJM | 30368956 CSFG |
| Title: | Functional screening pipeline to uncover laccase-like multicopper oxidase enzymes that transform industrial lignins | ||||
| Authors: | Sharan AA, Bellemare A, DiFalco M, Tsang A, Vuong TV, Edwards EA, Master ER | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/38000639/ | ||||
| DOI: | 10.1016/j.biortech.2023.130084 | ||||
| Publication: | Bioresource technology | ||||
| Keywords: | Kraft lignin; Laccase; Lignin-active enzymes; Organosolv lignin; ToF-SIMS; | ||||
| PMID: | 38000639 | Category: | Date Added: | 2023-11-25 | |
| Dept Affiliation: |
CSFG
1 Department of Chemical Engineering and Applied Chemistry, University of Toronto, Canada. 2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Canada. 3 Department of Chemical Engineering and Applied Chemistry, University of Toronto, Canada; Department of Cell and Systems Biology, University of Toronto, Canada. 4 Department of Chemical Engineering and Applied Chemistry, University of Toronto, Canada; Department of Bioproducts and Biosystems, Aalto University, Finland. Electronic address: emma.master@utoronto.ca. |
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Description: |
Laccase-like multicopper oxidases are recognized for their potential to alter the reactivity of lignins for application in value-added products. Typically, model compounds are employed to discover such enzymes; however, they do not represent the complexity of industrial lignin substrates. In this work, a screening pipeline was developed to test enzymes simultaneously on model compounds and industrial lignins. A total of 12 lignin-active fungal multicopper oxidases were discovered, including 9 enzymes active under alkaline conditions (pH 11.0). Principal component analysis revealed the poor ability of model compounds to predict enzyme performance on industrial lignins. Additionally, sequence similarity analyses grouped these enzymes with Auxiliary Activity-1 sub-families with few previously characterized members, underscoring their taxonomic novelty. Correlation between the lignin-activity of these enzymes and their taxonomic origin, however, was not observed. These are critical insights to bridge the gap between enzyme discovery and application for industrial lignin valorization. |
