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PubMed Publications| Keyword search (4,163 papers available) |  |
"Aspergillus" Keyword-tagged Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | An examination of the quinic acid utilization genes in Aspergillus niger reveals the involvement of two pH-dependent permeases | Sgro M; Reid ID; Arentshorst M; Ram AFJ; Tsang A; | 40853219 GENOMICS |
| 2 | Transcriptomics identify the triggering of citrate export as the key event caused by manganese deficiency in Aspergillus niger | Fekete E; Bíró V; Márton A; Bakondi-Kovács I; Sándor E; Kovács B; Geoffrion N; Tsang A; Kubicek CP; Karaffa L; | 39377610 CSFG |
| 3 | Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger | Sgro M; Chow N; Olyaei F; Arentshorst M; Geoffrion N; Ram AFJ; Powlowski J; Tsang A; | 37399977 BIOLOGY |
| 4 | Bioreactor as the root cause of the "manganese effect" during Aspergillus niger citric acid fermentations | Fekete E; Bíró V; Márton A; Bakondi-Kovács I; Németh Z; Sándor E; Kovács B; Fábián I; Kubicek CP; Tsang A; Karaffa L; | 35992333 CSFG |
| 5 | Lignocellulolytic enzymes from Aspergillus allahabadii for efficient bioconversion of rice straw into fermentable sugars and biogas | Sharma G; Kaur B; Raheja Y; Agrawal D; Basotra N; Di Falco M; Tsang A; Singh Chadha B; | 35753566 CSFG |
| 6 | The chimeric GaaR-XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger | Kun RS; Garrigues S; Di Falco M; Tsang A; de Vries RP; | 34236481 CSFG |
| 7 | Identification of a Novel Biosynthetic Gene Cluster in Aspergillus niger Using Comparative Genomics | Evdokias G; Semper C; Mora-Ochomogo M; Di Falco M; Nguyen TTM; Savchenko A; Tsang A; Benoit-Gelber I; | 34064722 BIOLOGY |
| 8 | Genetic Characterization of Mutations Related to Conidiophore Stalk Length Development in Aspergillus niger Laboratory Strain N402 | Demirci E; Arentshorst M; Yilmaz B; Swinkels A; Reid ID; Visser J; Tsang A; Ram AFJ; | 33959152 CSFG |
| 9 | Biosynthesis of Alkylcitric Acids in Aspergillus niger Involves Both Co-localized and Unlinked Genes. | Palys S, Pham TTM, Tsang A | 32695080 CSFG |
| 10 | Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans. | Bastos RW, Valero C, Silva LP, Schoen T, Drott M, Brauer V, Silva-Rocha R, Lind A, Steenwyk JL, Rokas A, Rodrigues F, Resendiz-Sharpe A, Lagrou K, Marcet-Houben M, Gabaldón T, McDonnell E, Reid I, Tsang A, Oakley BR, Loures FV, Almeida F, Huttenlocher A, Keller NP, Ries LNA, Goldman GH | 32269156 CSFG |
| 11 | The effects of external Mn2+ concentration on hyphal morphology and citric acid production are mediated primarily by the NRAMP-family transporter DmtA in Aspergillus niger. | Fejes B, Ouedraogo JP, Fekete E, Sándor E, Flipphi M, Soós Á, Molnár ÁP, Kovács B, Kubicek CP, Tsang A, Karaffa L | 32000778 CSFG |
| 12 | Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance. | Lubbers RJM, Liwanag AJ, Peng M, Dilokpimol A, Benoit-Gelber I, de Vries RP | 31674709 CSFG |
| 13 | Four Aromatic Intradiol Ring Cleavage Dioxygenases from Aspergillus niger. | Semana P, Powlowski J | 31540981 CHEMISTRY |
| 14 | Closely related fungi employ diverse enzymatic strategies to degrade plant biomass. | Benoit I, Culleton H, Zhou M, DiFalco M, Aguilar-Osorio G, Battaglia E, Bouzid O, Brouwer CPJM, El-Bushari HBO, Coutinho PM, Gruben BS, Hildén KS, Houbraken J, Barboza LAJ, Levasseur A, Majoor E, Mäkelä MR, Narang HM, Trejo-Aguilar B, van den Brink J, vanKuyk PA, Wiebenga A, McKie V, McCleary B, Tsang A, Henrissat B, de Vries RP | 26236396 CSFG |
| 15 | Expression-based clustering of CAZyme-encoding genes of Aspergillus niger. | Gruben BS, Mäkelä MR, Kowalczyk JE, Zhou M, Benoit-Gelber I, De Vries RP | 29169319 CSFG |
| 16 | W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger. | Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ | 30298571 CSFG |
| 17 | The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi. | Aguilar-Pontes MV, Brandl J, McDonnell E, Strasser K, Nguyen TTM, Riley R, Mondo S, Salamov A, Nybo JL, Vesth TC, Grigoriev IV, Andersen MR, Tsang A, de Vries RP | 30425417 CSFG |
| 18 | Genomic and exoproteomic diversity in plant biomass degradation approaches among Aspergilli | Mäkelä MR; DiFalco M; McDonnell E; Nguyen TTM; Wiebenga A; Hildén K; Peng M; Grigoriev IV; Tsang A; de Vries RP; | 30487660 CSFG |
| 19 | The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum. | Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP | 30797054 CSFG |
| Title: | The chimeric GaaR-XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger | ||||
| Authors: | Kun RS, Garrigues S, Di Falco M, Tsang A, de Vries RP | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/34236481/ | ||||
| DOI: | 10.1007/s00253-021-11428-2 | ||||
| Publication: | Applied microbiology and biotechnology | ||||
| Keywords: | Aspergillus niger; CRISPR/Cas9; Chimeric transcription factor; D-xylose; Pectinases; | ||||
| PMID: | 34236481 | Category: | Date Added: | 2021-07-08 | |
| Dept Affiliation: |
CSFG
1 Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584, CT, Utrecht, The Netherlands. 2 Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec, H4B 1R6, Canada. 3 Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584, CT, Utrecht, The Netherlands. r.devries@wi.knaw.nl. |
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Description: |
Aspergillus niger is a filamentous fungus well known for its ability to produce a wide variety of pectinolytic enzymes, which have many applications in the industry. The transcriptional activator GaaR is induced by 2-keto-3-deoxy-L-galactonate, a compound derived from D-galacturonic acid, and plays a major role in the regulation of pectinolytic genes. The requirement for inducer molecules can be a limiting factor for the production of enzymes. Therefore, the generation of chimeric transcription factors able to activate the expression of pectinolytic genes by using underutilized agricultural residues would be highly valuable for industrial applications. In this study, we used the CRISPR/Cas9 system to generate three chimeric GaaR-XlnR transcription factors expressed by the xlnR promoter by swapping the N-terminal region of the xylanolytic regulator XlnR to that of the GaaR in A. niger. As a test case, we constructed a PpgaX-hph reporter strain to evaluate the alteration of transcription factor specificity in the chimeric mutants. Our results showed that the chimeric GaaR-XlnR transcription factor was induced in the presence of D-xylose. Additionally, we generated a constitutively active GaaR-XlnR V756F version of the most efficient chimeric transcription factor to better assess its activity. Proteomics analysis confirmed the production of several pectinolytic enzymes by ?gaaR mutants carrying the chimeric transcription factor. This correlates with the improved release of D-galacturonic acid from pectin by the GaaR-XlnR V756F mutant, as well as by the increased L-arabinose release from the pectin side chains by both chimeric mutants under inducing condition, which is required for efficient degradation of pectin. KEY POINTS: • Chimeric transcription factors were generated by on-site mutations using CRISPR/Cas9. • PpgaX-hph reporter strain allowed for the screening of functional GaaR-XlnR mutants. • Chimeric GaaR-XlnR induced pectinolytic activities in the presence of D-xylose. |
