Keyword search (4,163 papers available)

"Biotechnol Biofuels" Category Publications:

Title Authors PubMed ID
1 Evidence for ligninolytic activity of the ascomycete fungus Podospora anserina. van Erven G, Kleijn AF, Patyshakuliyeva A, Di Falco M, Tsang A, de Vries RP, van Berkel WJH, Kabel MA 32322305
CSFG
2 The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor. Pereira EO, Tsang A, McAllister TA, Menassa R 23915965
CSFG
3 Deconstructing the genetic basis of spent sulphite liquor tolerance using deep sequencing of genome-shuffled yeast. Pinel D, Colatriano D, Jiang H, Lee H, Martin VJ 25866561
CSFG
4 Closely related fungi employ diverse enzymatic strategies to degrade plant biomass. Benoit I, Culleton H, Zhou M, DiFalco M, Aguilar-Osorio G, Battaglia E, Bouzid O, Brouwer CPJM, El-Bushari HBO, Coutinho PM, Gruben BS, Hildén KS, Houbraken J, Barboza LAJ, Levasseur A, Majoor E, Mäkelä MR, Narang HM, Trejo-Aguilar B, van den Brink J, vanKuyk PA, Wiebenga A, McKie V, McCleary B, Tsang A, Henrissat B, de Vries RP 26236396
CSFG
5 Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae. Larue K, Melgar M, Martin VJ 26949413
CSFG
6 Determinants of selection in yeast evolved by genome shuffling. Biot-Pelletier D, Pinel D, Larue K, Martin VJJ 30356826
CSFG

 

Title:Deconstructing the genetic basis of spent sulphite liquor tolerance using deep sequencing of genome-shuffled yeast.
Authors:Pinel D,  Colatriano D,  Jiang H,  Lee H,  Martin VJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/25866561?dopt=Abstract
DOI:10.1186/s13068-015-0241-z
Publication:Biotechnology for biofuels
Keywords:Complex trait;  Evolutionary engineering;  Genome shuffling;  Reverse engineering;  Tolerance;  Yeast; 
PMID:25866561 Category:Biotechnol Biofuels Date Added:2019-06-07
Dept Affiliation: CSFG
1 Department of Biology, Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec H4B 1R6 Canada ; Current address: Energy Biosciences Institute, University of California, Berkeley, Berkeley, CA 94704 USA.
2 Department of Biology, Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec H4B 1R6 Canada.
3 Department of Biology, Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec H4B 1R6 Canada ; Current address: Crabtree Nutrition Laboratories, McGill University Health Center, Montreal, Quebec H3A 1A1 Canada.
4 School of Environmental Sciences, University of Guelph, Guelph, Ontario N1G 2 W1 Canada.

Description:

Deconstructing the genetic basis of spent sulphite liquor tolerance using deep sequencing of genome-shuffled yeast.

Biotechnol Biofuels. 2015;8:53

Authors: Pinel D, Colatriano D, Jiang H, Lee H, Martin VJ

Abstract

BACKGROUND: Identifying the genetic basis of complex microbial phenotypes is currently a major barrier to our understanding of multigenic traits and our ability to rationally design biocatalysts with highly specific attributes for the biotechnology industry. Here, we demonstrate that strain evolution by meiotic recombination-based genome shuffling coupled with deep sequencing can be used to deconstruct complex phenotypes and explore the nature of multigenic traits, while providing concrete targets for strain development.

RESULTS: We determined genomic variations found within Saccharomyces cerevisiae previously evolved in our laboratory by genome shuffling for tolerance to spent sulphite liquor. The representation of these variations was backtracked through parental mutant pools and cross-referenced with RNA-seq gene expression analysis to elucidate the importance of single mutations and key biological processes that play a role in our trait of interest. Our findings pinpoint novel genes and biological determinants of lignocellulosic hydrolysate inhibitor tolerance in yeast. These include the following: protein homeostasis constituents, including Ubp7p and Art5p, related to ubiquitin-mediated proteolysis; stress response transcriptional repressor, Nrg1p; and NADPH-dependent glutamate dehydrogenase, Gdh1p. Reverse engineering a prominent mutation in ubiquitin-specific protease gene UBP7 in a laboratory S. cerevisiae strain effectively increased spent sulphite liquor tolerance.

CONCLUSIONS: This study advances understanding of yeast tolerance mechanisms to inhibitory substrates and biocatalyst design for a biomass-to-biofuel/biochemical industry, while providing insights into the process of mutation accumulation that occurs during genome shuffling.

PMID: 25866561 [PubMed]




BookR developed by Sriram Narayanan
for the Concordia University School of Health
Copyright © 2011-2026
Cookie settings
Concordia University