Keyword search (4,163 papers available)

"Biotechnol Biofuels" Category Publications:

Title Authors PubMed ID
1 Evidence for ligninolytic activity of the ascomycete fungus Podospora anserina. van Erven G, Kleijn AF, Patyshakuliyeva A, Di Falco M, Tsang A, de Vries RP, van Berkel WJH, Kabel MA 32322305
CSFG
2 The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor. Pereira EO, Tsang A, McAllister TA, Menassa R 23915965
CSFG
3 Deconstructing the genetic basis of spent sulphite liquor tolerance using deep sequencing of genome-shuffled yeast. Pinel D, Colatriano D, Jiang H, Lee H, Martin VJ 25866561
CSFG
4 Closely related fungi employ diverse enzymatic strategies to degrade plant biomass. Benoit I, Culleton H, Zhou M, DiFalco M, Aguilar-Osorio G, Battaglia E, Bouzid O, Brouwer CPJM, El-Bushari HBO, Coutinho PM, Gruben BS, Hildén KS, Houbraken J, Barboza LAJ, Levasseur A, Majoor E, Mäkelä MR, Narang HM, Trejo-Aguilar B, van den Brink J, vanKuyk PA, Wiebenga A, McKie V, McCleary B, Tsang A, Henrissat B, de Vries RP 26236396
CSFG
5 Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae. Larue K, Melgar M, Martin VJ 26949413
CSFG
6 Determinants of selection in yeast evolved by genome shuffling. Biot-Pelletier D, Pinel D, Larue K, Martin VJJ 30356826
CSFG

 

Title:Determinants of selection in yeast evolved by genome shuffling.
Authors:Biot-Pelletier D,  Pinel D,  Larue K,  Martin VJJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/30356826?dopt=Abstract
DOI:10.1186/s13068-018-1283-9
Publication:Biotechnology for biofuels
Keywords:Evolution;  Evolutionary engineering;  Genome shuffling;  Lignocellulosic hydrolysate tolerance;  Stress tolerance in yeast; 
PMID:30356826 Category:Biotechnol Biofuels Date Added:2019-06-07
Dept Affiliation: CSFG
1 1Department of Biology, Centre for Structural and Functional Genomics, Centre for Applied Synthetic Biology, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6 Canada.
2 2Present Address: Lallemand Inc., Montréal, QC H4P 2R2 Canada.
3 3Present Address: Amyris Inc, Emeryville, CA 94608 USA.
4 Present Address: Charles River Laboratories, Senneville, QC H9X 3R3 Canada.

Description:

Determinants of selection in yeast evolved by genome shuffling.

Biotechnol Biofuels. 2018;11:282

Authors: Biot-Pelletier D, Pinel D, Larue K, Martin VJJ

Abstract

Background: Genome shuffling (GS) is a widely adopted methodology for the evolutionary engineering of desirable traits in industrially relevant microorganisms. We have previously used genome shuffling to generate a strain of Saccharomyces cerevisiae that is tolerant to the growth inhibitors found in a lignocellulosic hydrolysate. In this study, we expand on previous work by performing a population-wide genomic survey of our genome shuffling experiment and dissecting the molecular determinants of the evolved phenotype.

Results: Whole population whole-genome sequencing was used to survey mutations selected during the experiment and extract allele frequency time series. Using growth curve assays on single point mutants and backcrossed derivatives, we explored the genetic architecture of the selected phenotype and detected examples of epistasis. Our results reveal cohorts of strongly correlated mutations, suggesting prevalent genetic hitchhiking and the presence of pre-existing founder mutations. From the patterns of apparent selection and the results of direct phenotypic assays, our results identify key driver mutations and deleterious hitchhikers.

Conclusions: We use these data to propose a model of inhibitor tolerance in our GS mutants. Our results also suggest a role for compensatory evolution and epistasis in our genome shuffling experiment and illustrate the impact of historical contingency on the outcomes of evolutionary engineering.

PMID: 30356826 [PubMed]




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