PERFORM Publications

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Title		Authors	PubMed ID
1	Nonphotochemical Spectral Hole Burning Study of Modified LH2 Complex from em Rbl. acidophilus /em : Do Parts of the Pigment Molecule Affect Small Light-Induced Structural Changes?	Levenberg A; Trempe A; Lujan MA; Picorel R; Zazubovich V;	41802209 PHYSICS
2	Rigorous Quantum-Mechanical Modeling of Tunneling-Based Structural Changes Associated with Line Shifts in Optical Spectroscopy Experiments in Pigment-Protein Complexes	Eng-Michell J; Yi B; Tan X; Garashchuk S; Zazubovich V;	41662623 PHYSICS
3	High-Resolution Frequency-Domain Spectroscopic and Modeling Studies of Photosystem I (PSI), PSI Mutants and PSI Supercomplexes	Zazubovich V; Jankowiak R;	38612659 PHYSICS
4	Identification of Residues Potentially Involved in Optical Shifts in the Water-Soluble Chlorophyll a-Binding Protein through Molecular Dynamics Simulations	Mai M; Zazubovich V; Mansbach RA;	38299975 PHYSICS
5	Frequency-Domain Spectroscopic Study of the Photosystem I Supercomplexes, Isolated IsiA Monomers, and the Intact IsiA Ring	Reinot T; Khmelnitskiy A; Zazubovich V; Toporik H; Mazor Y; Jankowiak R;	36065077 PHYSICS
6	Functional analysis of low-grade glioma genetic variants predicts key target genes and transcription factors.	Manjunath M; Yan J; Youn Y; Drucker KL; Kollmeyer TM; McKinney AM; Zazubovich V; Zhang Y; Costello JF; Eckel-Passow J; Selvin PR; Jenkins RB; Song JS;	33130899 PHYSICS
7	Bound detergent molecules in bacterial reaction centers facilitate detection of tetryl explosive.	Modafferi D, Zazubovich V, Kálmán L	32632533 PHYSICS
8	Evidence of Simultaneous Spectral Hole Burning Involving Two Tiers of the Protein Energy Landscape in Cytochrome b6f.	Shafiei G, Levenberg A, Lujan MA, Picorel R, Zazubovich V	31763829 PHYSICS
9	How Well Does the Hole-Burning Action Spectrum Represent the Site-Distribution Function of the Lowest-Energy State in Photosynthetic Pigment-Protein Complexes?	Zazubovich V, Jankowiak R	31265294 CHEMISTRY
10	Low-temperature protein dynamics of the B800 molecules in the LH2 light-harvesting complex: spectral hole burning study and comparison with single photosynthetic complex spectroscopy.	Grozdanov D, Herascu N, Reinot T, Jankowiak R, Zazubovich V	20166717 PHYSICS
11	Parameters of the protein energy landscapes of several light-harvesting complexes probed via spectral hole growth kinetics measurements.	Herascu N, Najafi M, Amunts A, Pieper J, Irrgang KD, Picorel R, Seibert M, Zazubovich V	21391534 PHYSICS
12	Self-assembly and sensor response of photosynthetic reaction centers on screen-printed electrodes.	Bhalla V, Zazubovich V	22027137 PHYSICS
13	Effects of the distributions of energy or charge transfer rates on spectral hole burning in pigment-protein complexes at low temperatures.	Herascu N, Ahmouda S, Picorel R, Seibert M, Jankowiak R, Zazubovich V	22046956 PHYSICS
14	Spectral hole burning, recovery, and thermocycling in chlorophyll-protein complexes: distributions of barriers on the protein energy landscape.	Najafi M, Herascu N, Seibert M, Picorel R, Jankowiak R, Zazubovich V	22957798 PHYSICS
15	Modeling of various optical spectra in the presence of slow excitation energy transfer in dimers and trimers with weak interpigment coupling: FMO as an example.	Herascu N, Kell A, Acharya K, Jankowiak R, Blankenship RE, Zazubovich V	24506338 PHYSICS
16	On the Controversial Nature of the 825 nm Exciton Band in the FMO Protein Complex.	Kell A, Acharya K, Zazubovich V, Jankowiak R	26269993 PHYSICS
17	Fluorescence line narrowing and Δ-FLN spectra in the presence of excitation energy transfer between weakly coupled chromophores.	Zazubovich V	25369116 PHYSICS
18	Conformational Changes in Pigment-Protein Complexes at Low Temperatures-Spectral Memory and a Possibility of Cooperative Effects.	Najafi M, Herascu N, Shafiei G, Picorel R, Zazubovich V	25985255 PHYSICS
19	Monte Carlo Modeling of Spectral Diffusion Employing Multiwell Protein Energy Landscapes: Application to Pigment-Protein Complexes Involved in Photosynthesis.	Najafi M, Zazubovich V	26020801 PHYSICS
20	On the Conflicting Estimations of Pigment Site Energies in Photosynthetic Complexes: A Case Study of the CP47 Complex.	Reinot T, Chen J, Kell A, Jassas M, Robben KC, Zazubovich V, Jankowiak R	27279733 PHYSICS
21	A simple and efficient method to prepare pure dimers and monomers of the cytochrome b 6 f complex from spinach.	Luján MA, Lorente P, Zazubovich V, Picorel R	28374305 PHYSICS
22	Probing Energy Landscapes of Cytochrome b6f with Spectral Hole Burning: Effects of Deuterated Solvent and Detergent.	Levenberg A, Shafiei G, Lujan MA, Giannacopoulos S, Picorel R, Zazubovich V	28956922 PHYSICS
23	Spectral Hole Burning in Cyanobacterial Photosystem I with P700 in Oxidized and Neutral States.	Herascu N, Hunter MS, Shafiei G, Najafi M, Johnson TW, Fromme P, Zazubovich V	27661089 CHEMBIOCHEM

Title:	A simple and efficient method to prepare pure dimers and monomers of the cytochrome b 6 f complex from spinach.
Authors:	Luján MA, Lorente P, Zazubovich V, Picorel R
Link:	https://www.ncbi.nlm.nih.gov/pubmed/28374305?dopt=Abstract
Publication:
Keywords:
PMID:	28374305	Category:	Photosynth Res	Date Added:	2019-06-04
Dept Affiliation:	PHYSICS 1 CSIC-Estación Experimental de Aula Dei, Avda. Montañana 1005, 50059, Zaragoza, Spain. 2 Department of Physics, Concordia University, 7141 Sherbrook Str. West, Montreal, QC, H4B 1R6, Canada. 3 CSIC-Estación Experimental de Aula Dei, Avda. Montañana 1005, 50059, Zaragoza, Spain. picorel@eead.csic.es.

Description:

A simple and efficient method to prepare pure dimers and monomers of the cytochrome b 6 f complex from spinach.

Photosynth Res. 2017 Jun;132(3):305-309

Authors: Luján MA, Lorente P, Zazubovich V, Picorel R

Abstract

Using a single size-exclusion chromatography we were able to isolate highly pure dimers and monomers of the Cyt b 6 f complex from spinach from a bulk preparation of that protein complex obtained with a standard procedure. At higher protein/detergent ratio during the chromatography most of the Cyt b 6 f complex remained as dimers. In contrast, at lower protein/detergent ratio (around 15 times lower), most dimers became monomerized. As a bonus, this chromatography also allowed the elimination of potential Chl a contaminant to the Cyt b 6 f preparations. SDS-PAGE protein analysis with 18% (w/v) acrylamide revealed the loss of the ISP subunit in our monomeric preparation. However, it fully retained the content of Chl a, a prerequisite to perform any spectroscopic study involving this unique pigment.

PMID: 28374305 [PubMed - indexed for MEDLINE]

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