Keyword search (4,163 papers available)

"Powlowski J" Authored Publications:

Title Authors PubMed ID
1 Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization in Aspergillus niger Arentshorst M; Falco MD; Moisan MC; Reid ID; Spaapen TOM; van Dam J; Demirci E; Powlowski J; Punt PJ; Tsang A; Ram AFJ; 37744122
CSFG
2 Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger Sgro M; Chow N; Olyaei F; Arentshorst M; Geoffrion N; Ram AFJ; Powlowski J; Tsang A; 37399977
BIOLOGY
3 Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls Derba-Maceluch M; Mitra M; Hedenström M; Liu X; Gandla ML; Barbut FR; Abreu IN; Donev EN; Urbancsok J; Moritz T; Jönsson LJ; Tsang A; Powlowski J; Master ER; Mellerowicz EJ; 36600379
CSFG
4 Carbohydrate esterase family 16 contains fungal hemicellulose acetyl esterases (HAEs) with varying specificity Venegas FA; Koutaniemi S; Langeveld SMJ; Bellemare A; Chong SL; Dilokpimol A; Lowden MJ; Hilden KS; Leyva-Illades JF; Mäkelä MR; My Pham TT; Peng M; Hancock MA; Zheng Y; Tsang A; Tenkanen M; Powlowski J; de Vries RP; 35405333
CSFG
5 Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases Dilokpimol A; Verkerk B; Li X; Bellemare A; Lavallee M; Frommhagen M; Nørmølle Underlin E; Kabel MA; Powlowski J; Tsang A; de Vries RP; 35187647
CSFG
6 Four Aromatic Intradiol Ring Cleavage Dioxygenases from Aspergillus niger. Semana P, Powlowski J 31540981
CHEMISTRY
7 Characterization of active and inactive forms of the phenol hydroxylase stimulatory protein DmpM. Cadieux E, Powlowski J 10451366
CHEMBIOCHEM
8 Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor. Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L 17333176
BIOLOGY
9 A shared binding site for NAD+ and coenzyme A in an acetaldehyde dehydrogenase involved in bacterial degradation of aromatic compounds. Lei Y, Pawelek PD, Powlowski J 18537268
CHEMBIOCHEM
10 Analytical and computational approaches to define the Aspergillus niger secretome. Tsang A, Butler G, Powlowski J, Panisko EA, Baker SE 19618504
BIOLOGY
11 A molecular phylogeny of thermophilic fungi. Morgenstern I, Powlowski J, Ishmael N, Darmond C, Marqueteau S, Moisan MC, Quenneville G, Tsang A 22483047
CSFG
12 Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila. Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A 24881579
BIOLOGY
13 mycoCLAP, the database for characterized lignocellulose-active proteins of fungal origin: resource and text mining curation support. Strasser K, McDonnell E, Nyaga C, Wu M, Wu S, Almeida H, Meurs MJ, Kosseim L, Powlowski J, Butler G, Tsang A 25754864
CSFG
14 Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting. Badhan A, Wang YX, Gruninger R, Patton D, Powlowski J, Tsang A, McAllister TA 26180803
CSFG

 

Title:Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting.
Authors:Badhan AWang YXGruninger RPatton DPowlowski JTsang AMcAllister TA
Link:https://www.ncbi.nlm.nih.gov/pubmed/26180803?dopt=Abstract
DOI:10.1155/2015/562952
Publication:BioMed research international
Keywords:
PMID:26180803 Category:Biomed Res Int Date Added:2019-06-07
Dept Affiliation: CSFG
1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada T1H 4P4.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada H4B 1R6.

Description:

Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting.

Biomed Res Int. 2015;2015:562952

Authors: Badhan A, Wang YX, Gruninger R, Patton D, Powlowski J, Tsang A, McAllister TA

Abstract

Identification of recalcitrant factors that limit digestion of forages and the development of enzymatic approaches that improve hydrolysis could play a key role in improving the efficiency of meat and milk production in ruminants. Enzyme fingerprinting of barley silage fed to heifers and total tract indigestible fibre residue (TIFR) collected from feces was used to identify cell wall components resistant to total tract digestion. Enzyme fingerprinting results identified acetyl xylan esterases as key to the enhanced ruminal digestion. FTIR analysis also suggested cross-link cell wall polymers as principal components of indigested fiber residues in feces. Based on structural information from enzymatic fingerprinting and FTIR, enzyme pretreatment to enhance glucose yield from barley straw and alfalfa hay upon exposure to mixed rumen-enzymes was developed. Prehydrolysis effects of recombinant fungal fibrolytic hydrolases were analyzed using microassay in combination with statistical experimental design. Recombinant hemicellulases and auxiliary enzymes initiated degradation of plant structural polysaccharides upon application and improved the in vitro saccharification of alfalfa and barley straw by mixed rumen enzymes. The validation results showed that microassay in combination with statistical experimental design can be successfully used to predict effective enzyme pretreatments that can enhance plant cell wall digestion by mixed rumen enzymes.

PMID: 26180803 [PubMed - indexed for MEDLINE]





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