Keyword search (4,163 papers available)

"Powlowski J" Authored Publications:

Title Authors PubMed ID
1 Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization in Aspergillus niger Arentshorst M; Falco MD; Moisan MC; Reid ID; Spaapen TOM; van Dam J; Demirci E; Powlowski J; Punt PJ; Tsang A; Ram AFJ; 37744122
CSFG
2 Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger Sgro M; Chow N; Olyaei F; Arentshorst M; Geoffrion N; Ram AFJ; Powlowski J; Tsang A; 37399977
BIOLOGY
3 Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls Derba-Maceluch M; Mitra M; Hedenström M; Liu X; Gandla ML; Barbut FR; Abreu IN; Donev EN; Urbancsok J; Moritz T; Jönsson LJ; Tsang A; Powlowski J; Master ER; Mellerowicz EJ; 36600379
CSFG
4 Carbohydrate esterase family 16 contains fungal hemicellulose acetyl esterases (HAEs) with varying specificity Venegas FA; Koutaniemi S; Langeveld SMJ; Bellemare A; Chong SL; Dilokpimol A; Lowden MJ; Hilden KS; Leyva-Illades JF; Mäkelä MR; My Pham TT; Peng M; Hancock MA; Zheng Y; Tsang A; Tenkanen M; Powlowski J; de Vries RP; 35405333
CSFG
5 Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases Dilokpimol A; Verkerk B; Li X; Bellemare A; Lavallee M; Frommhagen M; Nørmølle Underlin E; Kabel MA; Powlowski J; Tsang A; de Vries RP; 35187647
CSFG
6 Four Aromatic Intradiol Ring Cleavage Dioxygenases from Aspergillus niger. Semana P, Powlowski J 31540981
CHEMISTRY
7 Characterization of active and inactive forms of the phenol hydroxylase stimulatory protein DmpM. Cadieux E, Powlowski J 10451366
CHEMBIOCHEM
8 Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor. Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L 17333176
BIOLOGY
9 A shared binding site for NAD+ and coenzyme A in an acetaldehyde dehydrogenase involved in bacterial degradation of aromatic compounds. Lei Y, Pawelek PD, Powlowski J 18537268
CHEMBIOCHEM
10 Analytical and computational approaches to define the Aspergillus niger secretome. Tsang A, Butler G, Powlowski J, Panisko EA, Baker SE 19618504
BIOLOGY
11 A molecular phylogeny of thermophilic fungi. Morgenstern I, Powlowski J, Ishmael N, Darmond C, Marqueteau S, Moisan MC, Quenneville G, Tsang A 22483047
CSFG
12 Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila. Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A 24881579
BIOLOGY
13 mycoCLAP, the database for characterized lignocellulose-active proteins of fungal origin: resource and text mining curation support. Strasser K, McDonnell E, Nyaga C, Wu M, Wu S, Almeida H, Meurs MJ, Kosseim L, Powlowski J, Butler G, Tsang A 25754864
CSFG
14 Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting. Badhan A, Wang YX, Gruninger R, Patton D, Powlowski J, Tsang A, McAllister TA 26180803
CSFG

 

Title:Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor.
Authors:Lahjouji KStorms RXiao ZJoung KBZheng YPowlowski JTsang AVarin L
Link:https://www.ncbi.nlm.nih.gov/pubmed/17333176?dopt=Abstract
DOI:10.1007/s00253-006-0824-5
Publication:Applied microbiology and biotechnology
Keywords:
PMID:17333176 Category:Appl Microbiol Biotechnol Date Added:2019-06-20
Dept Affiliation: BIOLOGY
1 Centre for Structural and Functional Genomics, Biology Department, Concordia University, 7141 Sherbrooke street West, Montréal, Quebec, H4B 1R6, Canada.

Description:

Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor.

Appl Microbiol Biotechnol. 2007 May;75(2):337-46

Authors: Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L

Abstract

A cellobiohydrolase-encoding cDNA, Tvcel7a, from Trametes versicolor has been cloned and expressed in Aspergillus niger. The deduced amino acid sequence shows that Tvcel7a encodes a 456-amino acid polypeptide belonging to glycosyl hydrolase family 7. TvCel7a possesses a 19-amino acid secretion signal but does not possess a linker region nor a carbohydrate-binding domain. Two peaks of activity were obtained after TvCel7a was purified to apparent homogeneity by gel-filtration followed by anion-exchange chromatography. Mass spectrometry performed on the purified proteins confirmed that both peaks corresponded to the predicted sequence of the T. versicolor cellulase. The biochemical properties of the purified TvCel7a obtained from both peaks were studied in detail. The pH and temperature optima were 5.0 and 40 degrees C, respectively. The enzyme was stable over a pH range extending from pH 3.0 to 9.0 and at temperatures lower than 50 degrees C. The kinetic parameters with the substrate p-nitrophenyl beta-D: -cellobioside (pNPC) were 0.58 mM and 1.0 micromol/min/mg protein for the purified TvCel7a found in both peaks 1 and 2. TvCel7a catalyzes the hydrolysis of pNPC, filter paper, beta-glucan, and avicel to varying extents, but no detectable hydrolysis was observed when using the substrates carboxymethylcellulose, laminarin and pNPG.

PMID: 17333176 [PubMed - indexed for MEDLINE]





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