PERFORM Publications

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Title		Authors	PubMed ID
1	Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification	Raheja Y; Singh V; Gaur VK; Sharma G; Tsang A; Chadha BS;	40622460 GENOMICS
2	Heterologous Expression of Thermostable Endoglucanases from Rasamsonia emersonii: A Paradigm Shift in Biomass Hydrolysis	Raheja Y; Singh V; Gaur VK; Tsang A; Chadha BS;	40418313 GENOMICS
3	Retraction notice to "Thermostable xylanases from thermophilic fungi and bacteria: Current perspective" [Bioresour. Technol. 277 (2019) 195-203]	Chadha BS; Kaur B; Basotra N; Tsang A; Pandey A;	39447502 CSFG
4	Developing endophytic Penicillium oxalicum as a source of lignocellulolytic enzymes for enhanced hydrolysis of biorefinery relevant pretreated rice straw	Sharma G; Kaur B; Raheja Y; Kaur A; Singh V; Basotra N; Di Falco M; Tsang A; Chadha BS;	39249151 CSFG
5	Transcriptional and secretome analysis of Rasamsonia emersonii lytic polysaccharide mono-oxygenases	Raheja Y; Singh V; Kumar N; Agrawal D; Sharma G; Di Falco M; Tsang A; Chadha BS;	39167166 CSFG
6	Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass	Sharma G; Kaur B; Singh V; Raheja Y; Falco MD; Tsang A; Chadha BS;	38676717 CSFG
7	A thermostable and inhibitor resistant β-glucosidase from Rasamsonia emersonii for efficient hydrolysis of lignocellulosics biomass	Raheja Y; Singh V; Sharma G; Tsang A; Chadha BS;	38470501 CSFG
8	CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii	Singh V; Raheja Y; Basotra N; Sharma G; Tsang A; Chadha BS;	37658430 CSFG
9	Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum	Agrawal D; Tsang A; Chadha BS;	34293687 CSFG
10	Discovery and Expression of Thermostable LPMOs from Thermophilic Fungi for Producing Efficient Lignocellulolytic Enzyme Cocktails.	Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS	31792786 CSFG
11	Malbranchea cinnamomea: A thermophilic fungal source of catalytically efficient lignocellulolytic glycosyl hydrolases and metal dependent enzymes.	Mahajan C, Basotra N, Singh S, Di Falco M, Tsang A, Chadha BS	26476165 CSFG
12	Evaluation of secretome of highly efficient lignocellulolytic Penicillium sp. Dal 5 isolated from rhizosphere of conifers.	Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS	27341464 CSFG
13	Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics.	Basotra N, Joshi S, Satyanarayana T, Pati PK, Tsang A, Chadha BS	29174359 CSFG
14	Thermostable xylanases from thermophilic fungi and bacteria: Current perspective.	Chadha BS, Kaur B, Basotra N, Tsang A, Pandey A	30679061 CSFG
15	Characterization of a novel Lytic Polysaccharide Monooxygenase from Malbranchea cinnamomea exhibiting dual catalytic behavior	Basotra N; Dhiman SS; Agrawal D; Sani RK; Tsang A; Chadha BS;	31054382 ENCS

Title:	Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass
Authors:	Sharma G, Kaur B, Singh V, Raheja Y, Falco MD, Tsang A, Chadha BS
Link:	https://pubmed.ncbi.nlm.nih.gov/38676717/
DOI:	10.1007/s00203-024-03974-w
Publication:	Archives of microbiology
Keywords:	Myceliophthora verrucosa; CDH; Genome sequencing; Hydrolysis; LPMO; Secretome;
PMID:	38676717	Category:	Date Added:	2024-04-28
Dept Affiliation:	CSFG 1 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. 2 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada. 3 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. chadhabs@yahoo.com.

Description:

Lignocellulolytic enzymes from a novel Myceliophthora verrucosa (5DR) strain was found to potentiate the efficacy of benchmark cellulase during saccharification of acid/alkali treated bagasse by ~ 2.24 fold, indicating it to be an important source of auxiliary enzymes. The De-novo sequencing and analysis of M. verrucosa genome (31.7 Mb) revealed to encode for 7989 putative genes, representing a wide array of CAZymes (366) with a high proportions of auxiliary activity (AA) genes (76). The LC/MS QTOF based secretome analysis of M. verrucosa showed high abundance of glycosyl hydrolases and AA proteins with cellobiose dehydrogenase (CDH) (AA8), being the most prominent auxiliary protein. A gene coding for lytic polysaccharide monooxygenase (LPMO) was expressed in Pichia pastoris and CDH produced by M. verrucosa culture on rice straw based solidified medium were purified and characterized. The mass spectrometry of LPMO catalyzed hydrolytic products of avicel showed the release of both C1/C4 oxidized products, indicating it to be type-3. The lignocellulolytic cocktail comprising of in-house cellulase produced by Aspergillus allahabadii strain spiked with LPMO & CDH exhibited enhanced and better hydrolysis of mild alkali deacetylated (MAD) and unwashed acid pretreated rice straw slurry (UWAP), when compared to Cellic CTec3 at high substrate loading rate.

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