PERFORM Publications

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Title		Authors	PubMed ID
1	Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification	Raheja Y; Singh V; Gaur VK; Sharma G; Tsang A; Chadha BS;	40622460 GENOMICS
2	Heterologous Expression of Thermostable Endoglucanases from Rasamsonia emersonii: A Paradigm Shift in Biomass Hydrolysis	Raheja Y; Singh V; Gaur VK; Tsang A; Chadha BS;	40418313 GENOMICS
3	Retraction notice to "Thermostable xylanases from thermophilic fungi and bacteria: Current perspective" [Bioresour. Technol. 277 (2019) 195-203]	Chadha BS; Kaur B; Basotra N; Tsang A; Pandey A;	39447502 CSFG
4	Developing endophytic Penicillium oxalicum as a source of lignocellulolytic enzymes for enhanced hydrolysis of biorefinery relevant pretreated rice straw	Sharma G; Kaur B; Raheja Y; Kaur A; Singh V; Basotra N; Di Falco M; Tsang A; Chadha BS;	39249151 CSFG
5	Transcriptional and secretome analysis of Rasamsonia emersonii lytic polysaccharide mono-oxygenases	Raheja Y; Singh V; Kumar N; Agrawal D; Sharma G; Di Falco M; Tsang A; Chadha BS;	39167166 CSFG
6	Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass	Sharma G; Kaur B; Singh V; Raheja Y; Falco MD; Tsang A; Chadha BS;	38676717 CSFG
7	A thermostable and inhibitor resistant β-glucosidase from Rasamsonia emersonii for efficient hydrolysis of lignocellulosics biomass	Raheja Y; Singh V; Sharma G; Tsang A; Chadha BS;	38470501 CSFG
8	CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii	Singh V; Raheja Y; Basotra N; Sharma G; Tsang A; Chadha BS;	37658430 CSFG
9	Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum	Agrawal D; Tsang A; Chadha BS;	34293687 CSFG
10	Discovery and Expression of Thermostable LPMOs from Thermophilic Fungi for Producing Efficient Lignocellulolytic Enzyme Cocktails.	Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS	31792786 CSFG
11	Malbranchea cinnamomea: A thermophilic fungal source of catalytically efficient lignocellulolytic glycosyl hydrolases and metal dependent enzymes.	Mahajan C, Basotra N, Singh S, Di Falco M, Tsang A, Chadha BS	26476165 CSFG
12	Evaluation of secretome of highly efficient lignocellulolytic Penicillium sp. Dal 5 isolated from rhizosphere of conifers.	Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS	27341464 CSFG
13	Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics.	Basotra N, Joshi S, Satyanarayana T, Pati PK, Tsang A, Chadha BS	29174359 CSFG
14	Thermostable xylanases from thermophilic fungi and bacteria: Current perspective.	Chadha BS, Kaur B, Basotra N, Tsang A, Pandey A	30679061 CSFG
15	Characterization of a novel Lytic Polysaccharide Monooxygenase from Malbranchea cinnamomea exhibiting dual catalytic behavior	Basotra N; Dhiman SS; Agrawal D; Sani RK; Tsang A; Chadha BS;	31054382 ENCS

Title:	Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum
Authors:	Agrawal D, Tsang A, Chadha BS
Link:	https://pubmed.ncbi.nlm.nih.gov/34293687/
DOI:	10.1016/j.biortech.2021.125603
Publication:	Bioresource technology
Keywords:	Cloning; Feruloyl esterase; Hydrolysis; Scytalidium thermophilum; β; -xylosidase;
PMID:	34293687	Category:	Date Added:	2021-07-23
Dept Affiliation:	CSFG 1 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab-143005, India. 2 Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec H4B 1R6, Canada. 3 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab-143005, India. Electronic address: chadhabs@yahoo.com.

Description:

Two lignocellulolytic accessory enzymes, feruloyl esterase D (FAED_SCYTH) and ß-xylosidase (XYL43B_SCYTH) were cloned and produced in the Pichia pastoris X33 as host. The molecular weight of recombinant enzymes FAED_SCYTH and XYL43B_SCYTH were ~ 31 and 40 kDa, respectively. FAED_SCYTH showed optimal activity at pH 6.0, 60 °C; and XYL43B_SCYTH at pH 7.0, 50 °C. FAED_SCYTH and XYL43B_SCYTH exhibited t_1/2: 4 and 0.5 h, respectively (50 °C, pH 5.0). The ß-xylosidase was bi-functional with pronounced activity against pNP-a-arabinofuranoside besides being highly xylose tolerant (retaining ~ 97% activity in the presence of 700 mM xylose). Cocktails prepared using these enzymes along with AA9 protein (PMO9D_SCYTH) and commercial cellulase CellicCTec2, showed improved hydrolysis of the pre-treated lignocellulosic biomass. Priming of pre-treated lignocellulosic biomass with these accessory enzymes was found to further enhance the hydrolytic potential of CellicCTec2 promising to reduce the enzyme load and cost required for obtaining sugars from biorefinery relevant pre-treated substrates.

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