1 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Metabolism, Obesity, Nutrition Lab, PERFORM Centre, Concordia University, 7200 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Centre de Recherche - Axe Maladies Chroniques, Centre Intégré Universitaire de Santé et de Services Sociaux Du Nord-de-l'Ile-de-Montreal, Hôpital Du Sacré-Coeur de Montreal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada.
2 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Division of Geriatric Medicine, McGill University Health Centre - Royal Victoria Hospital, 1001, Décarie, Montreal, Québec, H4A 3J1, Canada.
3 Departement Du Chirurgie, Hôpital Du Sacré-Coeur de Montréal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada.
4 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Metabolism, Obesity, Nutrition Lab, PERFORM Centre, Concordia University, 7200 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Centre de Recherche - Axe Maladies Chroniques, Centre Intégré Universitaire de Santé et de Services Sociaux Du Nord-de-l'Ile-de-Montreal, Hôpital Du Sacré-Coeur de Montreal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada. Electronic address: s.santosa@concordia.ca.

A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.

Anal Biochem. 2020 Sep 11; :113951

Authors: Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S

Abstract

The ability to accurately identify and quantify immune cell populations within adipose tissue is important in understanding the role of immune cells in metabolic disease risk. Flow cytometry is the gold standard method for immune cell quantification. However, quantification of immune cells from adipose tissue presents a number of challenges because of the complexities of working with an oily substance and the rapid deterioration of immune cell viability before analysis can be performed. Here we present a highly reproducible flow cytometry protocol for the quantification of immune cells in human adipose tissue, which overcomes these issues.

PMID: 32926866 [PubMed - as supplied by publisher]