Keyword search (4,163 papers available)

"xylanase" Keyword-tagged Publications:

Title Authors PubMed ID
1 Effect of ammonia fiber expansion-treated wheat straw and a recombinant fibrolytic enzyme on rumen microbiota and fermentation parameters, total tract digestibility, and performance of lambs. Ribeiro GO; Gruninger RJ; Jones DR; Beauchemin KA; Yang WZ; Wang Y; Abbott DW; Tsang A; McAllister TA; 32369600
CSFG
2 Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics. Basotra N, Joshi S, Satyanarayana T, Pati PK, Tsang A, Chadha BS 29174359
CSFG
3 Thermostable xylanases from thermophilic fungi and bacteria: Current perspective. Chadha BS, Kaur B, Basotra N, Tsang A, Pandey A 30679061
CSFG

 

Title:Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics.
Authors:Basotra NJoshi SSatyanarayana TPati PKTsang AChadha BS
Link:https://www.ncbi.nlm.nih.gov/pubmed/29174359?dopt=Abstract
DOI:10.1016/j.ijbiomac.2017.11.131
Publication:International journal of biological macromolecules
Keywords:Cellic CTec2Enhanced hydrolysisExpressionGH11)Malbranchea cinnamomeaPichia pastorisSynergismXylanase (GH10
PMID:29174359 Category:Int J Biol Macromol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Department of Microbiology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India. Electronic address: nehabasotra506@gmail.com.
2 Central University of Gujarat, Gandhinagar, Gujarat, India. Electronic address: swati.joshi.aj@gmail.com.
3 Division of Biological Sciences & Engineering, Netaji Subhas Institute of Technology, Azad Hind Fauz Marg, Dwarka, New Delhi,110078, India. Electronic address: tsnarayana@gmail.com.
4 Department of Biotechnology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India. Electronic address: pkpati@yahoo.com.
5 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec H4 B 1R6, Canada. Electronic address: adrian.tsang@concordia.ca.
6 Department of Microbiology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India. Electronic address: chadhabs@yahoo.com.

Description:

Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics.

Int J Biol Macromol. 2018 Mar;108:185-192

Authors: Basotra N, Joshi S, Satyanarayana T, Pati PK, Tsang A, Chadha BS

Abstract

In this study, two xylanase genes (GH10 and GH11) derived from Malbranchea cinnamomea, designated as XYN10A_MALCI and XYN11A_MALCI, respectively, were expressed in Pichia pastoris X33. The maximum level of xylanase expression was found to be 24.3U/ml for rXYN10A_MALCI and 573.32U/ml for rXYN11A_MALCI. The purified recombinant rXYN11A_MALCI was stable at 70°C and catalytically active against a variety of substituted (arabinoxylans) as well as unsubstituted xylans. The hydrolytic potential of recombinant xylanases for enhancing the hydrolysis of acid/alkali pretreated lignocellulosics (rice straw and bagasse) by the commercial cellulase Cellic CTec2 was assessed which revealed that both rXYN10A_MALCI and rXYN11A_MALCI act synergistically with commercial cellulases and resulted in 1.54 and 1.58 folds improved hydrolysis of acid treated rice straw and alkali treated rice straw using cocktail comprising of Cellic CTec2 and XYN11A_MALCI (8:2 ratio) when compared to Cellic CTec2 alone at same protein loading rate of (~5.7mg/g biomass).

PMID: 29174359 [PubMed - indexed for MEDLINE]





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