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PubMed Publications| Keyword search (4,163 papers available) |  |
"transcriptome" Keyword-tagged Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Geography, Ancestry, Age and Sex Shape Somatic Autosomal Mosaic Chromosomal Alterations in Blood | Won Kang JR; Kim YJ; Skead K; Soave D; Evans J; Bruat V; Harwood MP; Morris Q; Matovu E; Mulindwa J; Noyes H; McLeod A; Hazelhurst S; Lombard Z; Ramsay M; Fave MJ; Awadalla P; | 41282824 BIOLOGY |
| 2 | Comparative analysis of functional diversity of rumen microbiome in bison and beef heifers | Nguyen TTM; Badhan AK; Reid ID; Ribeiro G; Gruninger R; Tsang A; Guan LL; McAllister T; | 38054735 CSFG |
| 3 | The Sugar Metabolic Model of Aspergillus niger Can Only Be Reliably Transferred to Fungi of Its Phylum | Li J; Chroumpi T; Garrigues S; Kun RS; Meng J; Salazar-Cerezo S; Aguilar-Pontes MV; Zhang Y; Tejomurthula S; Lipzen A; Ng V; Clendinen CS; Tolic N; Grigoriev IV; Tsang A; Mäkelä MR; Snel B; Peng M; de Vries RP; | 36547648 BIOLOGY |
| 4 | Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST) | Roversi K; Ebrahimi Orimi H; Falchetti M; Lummertz da Rocha E; Talbot S; Boutopoulos C; | 34442487 ENCS |
| 5 | Penicillium subrubescens adapts its enzyme production to the composition of plant biomass. | Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP | 32408196 CSFG |
| 6 | Mining Enzyme Diversity of Transcriptome Libraries through DNA Synthesis for Benzylisoquinoline Alkaloid Pathway Optimization in Yeast. | Narcross L, Bourgeois L, Fossati E, Burton E, Martin VJ | 27442619 BIOLOGY |
| 7 | The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum. | Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP | 30797054 CSFG |
| Title: | Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST) | ||||
| Authors: | Roversi K, Ebrahimi Orimi H, Falchetti M, Lummertz da Rocha E, Talbot S, Boutopoulos C | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/34442487/ | ||||
| DOI: | 10.3390/mi12080865 | ||||
| Publication: | Micromachines | ||||
| Keywords: | adult DRG neurons; calcium kinetics; laser-assisted bioprinting; laser-induced side transfer; sensory neurons; transcriptome; viability; | ||||
| PMID: | 34442487 | Category: | Date Added: | 2021-08-27 | |
| Dept Affiliation: |
ENCS
1 Département de Pharmacologie et Physiologie, Université de Montréal, Montréal, QC H3C 3J7, Canada. 2 Centre de Recherche Hôpital Maisonneuve-Rosemont, Montréal, QC H1T 2M4, Canada. 3 Department of Mechanical, Industrial and Aerospace Engineering, Concordia University, Montréal, QC H3G 1M8, Canada. 4 Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, Brazil. 5 Département d'Ophtalmologie, Université de Montréal, Montréal, QC H3C 3J7, Canada. 6 Institut de Génie Biomédical, Université de Montréal, Montréal, QC H3C 3J7, Canada. |
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Description: |
Cell bioprinting technologies aim to fabricate tissuelike constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. Here, we sought to validate the feasibility of bioprinting primary adult sensory neurons using a newly developed laser-assisted cell bioprinting technology, known as Laser-Induced Side Transfer (LIST). We used dorsal root ganglion neurons (DRG; cell bodies of somatosensory neurons) to prepare our bioink. DRG-laden- droplets were printed on fibrin-coated coverslips and their viability, calcium kinetics, neuropeptides release, and neurite outgrowth were measured. The transcriptome of the neurons was sequenced. We found that LIST-printed neurons maintain high viability (Printed: 86%, Control: 87% on average) and their capacity to release neuropeptides (Printed CGRP: 130 pg/mL, Control CGRP: 146 pg/mL). In addition, LIST-printed neurons do not show differences in the expressed genes compared to control neurons. However, in printed neurons, we found compromised neurite outgrowth and lower sensitivity to the ligand of the TRPV1 channel, capsaicin. In conclusion, LIST-printed neurons maintain high viability and marginal functionality losses. Overall, this work paves the way for bioprinting functional 2D neuron assays. |
