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PubMed Publications| Keyword search (4,164 papers available) |  |
"synthetic biology" Keyword-tagged Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield | Narcross L; Pyne ME; Kevvai K; Siu KH; Dueber JE; Martin VJJ; | 41779670 BIOLOGY |
| 2 | A Bacteroides synthetic biology toolkit to build an in vivo malabsorption biosensor | McCallum G; Burckhardt JC; He J; Hong A; Potvin-Trottier L; Tropini C; | 41610848 BIOLOGY |
| 3 | Robustness and evolvability: Revisited, redefined and applied | Kharma N; Bédard-Couture R; | 39098381 ENCS |
| 4 | BioCloneBot: A versatile, low-cost, and open-source automated liquid handler | Wells KC; Kharma N; Jaunky BB; Nie K; Aguiar-Tawil G; Berry D; | 38524156 BIOLOGY |
| 5 | Functional Synthetic Biology | Aldulijan I; Beal J; Billerbeck S; Bouffard J; Chambonnier G; Ntelkis N; Guerreiro I; Holub M; Ross P; Selvarajah V; Sprent N; Vidal G; Vignoni A; | 37073284 BIOLOGY |
| 6 | Advancing Undergraduate Synthetic Biology Education: Insights from a Canadian iGEM Student Perspective | Diep P; Boucinha A; Kell BJ; Yeung BA; Chen XA; Tsyplenkov D; Serra D; Escobar A; Gnanapragasam A; Emond CA; Sajtovich VA; Mahadevan R; Kilkenny DM; Gini-Newman G; Kaern M; Ingalls B; | 34237221 BIOLOGY |
| 7 | Using Models to (Re-)Design Synthetic Circuits. | McCallum G, Potvin-Trottier L | 33405217 BIOLOGY |
| 8 | Computer-Aided Design of Active Pseudoknotted Hammerhead Ribozymes. | Najeh S, Zandi K, Djerroud S, Kharma N, Perreault J | 32712917 ENCS |
| 9 | Reconstituting Plant Secondary Metabolism in Saccharomyces cerevisiae for Production of High-Value Benzylisoquinoline Alkaloids. | Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ | 27417930 CSFG |
| Title: | A Bacteroides synthetic biology toolkit to build an in vivo malabsorption biosensor | ||||
| Authors: | McCallum G, Burckhardt JC, He J, Hong A, Potvin-Trottier L, Tropini C | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/41610848/ | ||||
| DOI: | 10.1016/j.cell.2025.12.052 | ||||
| Publication: | Cell | ||||
| Keywords: | Bacteroides; Bacteroides thetaiotaomicron transcriptomics; commensal bacterium synthetic biology; fluorescent protein reporters; gut biosensor; inducible promoters; malabsorption; repressor sponging; transcriptional reporters; | ||||
| PMID: | 41610848 | Category: | Date Added: | 2026-01-30 | |
| Dept Affiliation: |
BIOLOGY
1 Department of Microbiology & Immunology, University of British Columbia, Vancouver, BC, Canada. 2 School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada. 3 Department of Biology, Concordia University, Montréal, QC, Canada; Department of Physics, Concordia University, Montréal, QC, Canada; Center for Applied Synthetic Biology, Concordia University, Montréal, QC, Canada. 4 Department of Microbiology & Immunology, University of British Columbia, Vancouver, BC, Canada; School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada; Humans and the Microbiome Program, Canadian Institute for Advanced Research (CIFAR), Toronto, ON, Canada. Electronic address: carolina.tropini@ubc.ca. |
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Description: |
The human gut is a dynamic environment, where changes in pH, oxygen, and osmolality influence microbiota composition and disease. Monitoring these environmental shifts is crucial for advancing gut health diagnostics and therapeutics, yet non-invasive monitoring tools remain limited. Genetically tractable commensals, including Bacteroides thetaiotaomicron, offer promising chassis for engineering biosensors but lack modular systems for precise sensing and reporting. Here, we developed genetic tools for B. thetaiotaomicron, including (1) repressible promoters for tunable fluorescent protein expression, (2) a DNA-based system to modulate repressor activity, (3) a modular, fluorescence-based transcriptional reporter circuit, and (4) an alternative plasmid integration mode. Using these components, we engineered biosensors to detect increased gut osmolality caused by malabsorption and validated them in vitro and in a murine model of laxative-induced osmotic diarrhea. These biosensors enabled long-term, non-invasive reporting of gut osmolality from single-cell fluorescence, demonstrating the potential of gut bacteria as monitoring platforms in gut health applications. |
