PERFORM Publications

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Title		Authors	PubMed ID
1	Attention, working memory, and inhibitory control in aging: Comparing amateur singers, instrumentalists, and active controls	Joyal M; Sicard A; Penhune V; Jackson PL; Tremblay P;	39367878 PSYCHOLOGY
2	Machine Learning-Assisted Short-Wave InfraRed (SWIR) Techniques for Biomedical Applications: Towards Personalized Medicine	Salimi M; Roshanfar M; Tabatabaei N; Mosadegh B;	38248734 ENCS
3	Comparative analysis of functional diversity of rumen microbiome in bison and beef heifers	Nguyen TTM; Badhan AK; Reid ID; Ribeiro G; Gruninger R; Tsang A; Guan LL; McAllister T;	38054735 CSFG
4	Infrared Thermography-A Novel Tool for Monitoring Fracture Healing: A Critically Appraised Topic With Evidence-Based Recommendations for Clinical Practice	Castonguay T; Dover G;	37433522 PERFORM
5	Effect of ammonia fiber expansion-treated wheat straw and a recombinant fibrolytic enzyme on rumen microbiota and fermentation parameters, total tract digestibility, and performance of lambs.	Ribeiro GO; Gruninger RJ; Jones DR; Beauchemin KA; Yang WZ; Wang Y; Abbott DW; Tsang A; McAllister TA;	32369600 CSFG
6	Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance and total tract digestibility of heifers fed a high forage diet.	Ran T, Saleem AM, Shen Y, Ribeiro GO, Beauchemin KA, Tsang A, Yang W, McAllister TA	31251799 CSFG
7	Discovery and characterization of family 39 glycoside hydrolases from rumen anaerobic fungi with polyspecific activity on rare arabinosyl substrates.	Jones DR, Uddin MS, Gruninger RJ, Pham TTM, Thomas D, Boraston AB, Briggs J, Pluvinage B, McAllister TA, Forster RJ, Tsang A, Selinger LB, Abbott DW	28588026 CSFG
8	Identification of novel enzymes to enhance the ruminal digestion of barley straw	Badhan A; Ribeiro GO; Jones DR; Wang Y; Abbott DW; Di Falco M; Tsang A; McAllister TA;	29621684 CSFG
9	New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw.	Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA	30053012 CSFG
10	Application of Transcriptomics to Compare the Carbohydrate Active Enzymes That Are Expressed by Diverse Genera of Anaerobic Fungi to Degrade Plant Cell Wall Carbohydrates.	Gruninger RJ, Nguyen TTM, Reid ID, Yanke JL, Wang P, Abbott DW, Tsang A, McAllister T	30061875 CSFG

Title:	Identification of novel enzymes to enhance the ruminal digestion of barley straw
Authors:	Badhan A, Ribeiro GO, Jones DR, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister TA
Link:	https://pubmed.ncbi.nlm.nih.gov/29621684/
DOI:	10.1016/j.biortech.2018.03.086
Publication:	Bioresource technology
Keywords:	Blue Native PAGE; Carbohydrate active enzymes (CAZymes); Fiber utilization; Glycosyl hydrolase; LC-MS/MS; Rumen nutrition;
PMID:	29621684	Category:	Bioresour Technol	Date Added:	2019-06-07
Dept Affiliation:	CSFG 1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada. 2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec H4B 1R6, Canada. 3 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada. Electronic address: tim.mcallister@agr.gc.ca.

Description:

Crude enzyme extracts typically contain a broad spectrum of enzyme activities, most of which are redundant to those naturally produced by the rumen microbiome. Identification of enzyme activities that are synergistic to those produced by the rumen microbiome could enable formulation of enzyme cocktails that improve fiber digestion in ruminants. Compared to untreated barley straw, Viscozyme® increased gas production, dry matter digestion (P < 0.01) and volatile fatty acid production (P < 0.001) in ruminal batch cultures. Fractionation of Viscozyme® by Blue Native PAGE and analyses using a microassay and mass-spectrometry revealed a GH74 endoglucanase, GH71 a-1,3-glucanase, GH5 mannanase, GH7 cellobiohydrolase, GH28 pectinase, and esterases from Viscozyme® contributed to enhanced saccharification of barley straw by rumen mix enzymes. Grouping of these identified activities with their carbohydrate active enzymes (CAZy) counterparts enabled selection of similar CAZymes for downstream production and screening. Mining of these specific activities from other biological systems could lead to high value enzyme formulations for ruminants.

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