Keyword search (4,163 papers available)

"pectinase" Keyword-tagged Publications:

Title Authors PubMed ID
1 The chimeric GaaR-XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger Kun RS; Garrigues S; Di Falco M; Tsang A; de Vries RP; 34236481
CSFG
2 The pathway intermediate 2-keto-3-deoxy-L-galactonate mediates the induction of genes involved in D-galacturonic acid utilization in Aspergillus niger. Alazi E, Khosravi C, Homan TG, du Pré S, Arentshorst M, Di Falco M, Pham TTM, Peng M, Aguilar-Pontes MV, Visser J, Tsang A, de Vries RP, Ram AFJ 28417461
CSFG
3 W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger. Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ 30298571
CSFG

 

Title:W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.
Authors:Alazi E,  Niu J,  Otto SB,  Arentshorst M,  Pham TTM,  Tsang A,  Ram AFJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/30298571?dopt=Abstract
DOI:10.1002/mbo3.732
Publication:MicrobiologyOpen
Keywords:Aspergillus niger;  CRISPR-Cas9;  constitutively active transcription factor;  missense mutation;  pectinase;  transcription factor localization; 
PMID:30298571 Category:Microbiologyopen Date Added:2019-06-07
Dept Affiliation: CSFG
1 Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Québec, Canada.

Description:

W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.

Microbiologyopen. 2019 May;8(5):e00732

Authors: Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ

Abstract

Polysaccharides present in plant biomass, such as pectin, are the main carbon source for filamentous fungi. Aspergillus niger naturally secretes pectinases to degrade pectin and utilize the released monomers, mainly D-galacturonic acid. The transcriptional activator GaaR, the repressor of D-galacturonic acid utilization GaaX, and the physiological inducer 2-keto-3-deoxy-L-galactonate play important roles in the transcriptional regulation of D-galacturonic acid-responsive genes, which include the genes encoding pectinases. In this study, we described the mutations found in gaaX and gaaR that enabled constitutive (i.e., inducer-independent) expression of pectinases by A. niger. Using promoter-reporter strains (PpgaX-amdS) and polygalacturonic acid plate assays, we showed that W361R mutation in GaaR results in constitutive production of pectinases. Analysis of subcellular localization of C-terminally eGFP-tagged GaaR/GaaRW 361R revealed important differences in nuclear accumulation of N- versus C-terminally eGFP-tagged GaaR.

PMID: 30298571 [PubMed - in process]




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