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PubMed Publications| Keyword search (4,163 papers available) |  |
"genome sequencing" Keyword-tagged Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Sequencing of a Dairy Isolate Unlocks em Kluyveromyces marxianus /em as a Host for Lactose Valorization | Thornbury M; Knoops A; Summerby-Murray I; Dhaliwal J; Johnson S; Utomo JC; Joshi J; Narcross L; Remondetto G; Pouliot M; Whiteway M; Martin VJJ; | 40629255 BIOLOGY |
| 2 | Widespread admixture blurs population structure and confounds Lake Trout (Salvelinus namaycush) conservation even in the genomic era | Bernos TA; Gibelli J; Michaelides S; Won H; Jeon HB; Marin K; Boguski DA; Janjua MY; Gallagher CP; Howland KL; Fraser DJ; | 39730611 BIOLOGY |
| 3 | Development of SNP Panels from Low-Coverage Whole Genome Sequencing (lcWGS) to Support Indigenous Fisheries for Three Salmonid Species in Northern Canada | Beemelmanns A; Bouchard R; Michaelides S; Normandeau E; Jeon HB; Chamlian B; Babin C; Hénault P; Perrot O; Harris LN; Zhu X; Fraser D; Bernatchez L; Moore JS; | 39552382 BIOLOGY |
| 4 | Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass | Sharma G; Kaur B; Singh V; Raheja Y; Falco MD; Tsang A; Chadha BS; | 38676717 CSFG |
| 5 | Community-led risk analysis of direct-to-consumer whole-genome sequencing | Samlali K; Thornbury M; Venter A; | 35939839 ENCS |
| 6 | Neutral and adaptive drivers of genomic change in introduced brook trout (Salvelinus fontinalis) populations revealed by pooled sequencing | Brookes B; Jeon HB; Derry AM; Post JR; Rogers SM; Humphries S; Fraser DJ; | 35154655 BIOLOGY |
| 7 | Genetic Characterization of Mutations Related to Conidiophore Stalk Length Development in Aspergillus niger Laboratory Strain N402 | Demirci E; Arentshorst M; Yilmaz B; Swinkels A; Reid ID; Visser J; Tsang A; Ram AFJ; | 33959152 CSFG |
| 8 | Genome Sequence Resource of Bacillus velezensis EB14, a native endophytic bacterial strain with biocontrol potential against the poplar stem canker causative pathogen, Sphaerulina musiva. | Naik S, Tsang A, Ramanan US, Dayanandan S | 33263425 BIOLOGY |
| 9 | Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans. | Bastos RW, Valero C, Silva LP, Schoen T, Drott M, Brauer V, Silva-Rocha R, Lind A, Steenwyk JL, Rokas A, Rodrigues F, Resendiz-Sharpe A, Lagrou K, Marcet-Houben M, Gabaldón T, McDonnell E, Reid I, Tsang A, Oakley BR, Loures FV, Almeida F, Huttenlocher A, Keller NP, Ries LNA, Goldman GH | 32269156 CSFG |
| Title: | Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass | ||||
| Authors: | Sharma G, Kaur B, Singh V, Raheja Y, Falco MD, Tsang A, Chadha BS | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/38676717/ | ||||
| DOI: | 10.1007/s00203-024-03974-w | ||||
| Publication: | Archives of microbiology | ||||
| Keywords: | Myceliophthora verrucosa; CDH; Genome sequencing; Hydrolysis; LPMO; Secretome; | ||||
| PMID: | 38676717 | Category: | Date Added: | 2024-04-28 | |
| Dept Affiliation: |
CSFG
1 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. 2 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada. 3 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. chadhabs@yahoo.com. |
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Description: |
Lignocellulolytic enzymes from a novel Myceliophthora verrucosa (5DR) strain was found to potentiate the efficacy of benchmark cellulase during saccharification of acid/alkali treated bagasse by ~ 2.24 fold, indicating it to be an important source of auxiliary enzymes. The De-novo sequencing and analysis of M. verrucosa genome (31.7 Mb) revealed to encode for 7989 putative genes, representing a wide array of CAZymes (366) with a high proportions of auxiliary activity (AA) genes (76). The LC/MS QTOF based secretome analysis of M. verrucosa showed high abundance of glycosyl hydrolases and AA proteins with cellobiose dehydrogenase (CDH) (AA8), being the most prominent auxiliary protein. A gene coding for lytic polysaccharide monooxygenase (LPMO) was expressed in Pichia pastoris and CDH produced by M. verrucosa culture on rice straw based solidified medium were purified and characterized. The mass spectrometry of LPMO catalyzed hydrolytic products of avicel showed the release of both C1/C4 oxidized products, indicating it to be type-3. The lignocellulolytic cocktail comprising of in-house cellulase produced by Aspergillus allahabadii strain spiked with LPMO & CDH exhibited enhanced and better hydrolysis of mild alkali deacetylated (MAD) and unwashed acid pretreated rice straw slurry (UWAP), when compared to Cellic CTec3 at high substrate loading rate. |
