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PubMed Publications| Keyword search (4,163 papers available) |  |
"feruloyl esterase" Keyword-tagged Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Utilization of ferulic acid in Aspergillus niger requires the transcription factor FarA and a newly identified Far-like protein (FarD) that lacks the canonical Zn(II)2Cys6 domain | Arentshorst M; Reijngoud J; van Tol DJC; Reid ID; Arendsen Y; Pel HJ; van Peij NNME; Visser J; Punt PJ; Tsang A; Ram AFJ; | 37746181 CSFG |
| 2 | Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases | Dilokpimol A; Verkerk B; Li X; Bellemare A; Lavallee M; Frommhagen M; Nørmølle Underlin E; Kabel MA; Powlowski J; Tsang A; de Vries RP; | 35187647 CSFG |
| 3 | Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum | Agrawal D; Tsang A; Chadha BS; | 34293687 CSFG |
| Title: | Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum | ||||
| Authors: | Agrawal D, Tsang A, Chadha BS | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/34293687/ | ||||
| DOI: | 10.1016/j.biortech.2021.125603 | ||||
| Publication: | Bioresource technology | ||||
| Keywords: | Cloning; Feruloyl esterase; Hydrolysis; Scytalidium thermophilum; β; -xylosidase; | ||||
| PMID: | 34293687 | Category: | Date Added: | 2021-07-23 | |
| Dept Affiliation: |
CSFG
1 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab-143005, India. 2 Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec H4B 1R6, Canada. 3 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab-143005, India. Electronic address: chadhabs@yahoo.com. |
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Description: |
Two lignocellulolytic accessory enzymes, feruloyl esterase D (FAED_SCYTH) and ß-xylosidase (XYL43B_SCYTH) were cloned and produced in the Pichia pastoris X33 as host. The molecular weight of recombinant enzymes FAED_SCYTH and XYL43B_SCYTH were ~ 31 and 40 kDa, respectively. FAED_SCYTH showed optimal activity at pH 6.0, 60 °C; and XYL43B_SCYTH at pH 7.0, 50 °C. FAED_SCYTH and XYL43B_SCYTH exhibited t1/2: 4 and 0.5 h, respectively (50 °C, pH 5.0). The ß-xylosidase was bi-functional with pronounced activity against pNP-a-arabinofuranoside besides being highly xylose tolerant (retaining ~ 97% activity in the presence of 700 mM xylose). Cocktails prepared using these enzymes along with AA9 protein (PMO9D_SCYTH) and commercial cellulase CellicCTec2, showed improved hydrolysis of the pre-treated lignocellulosic biomass. Priming of pre-treated lignocellulosic biomass with these accessory enzymes was found to further enhance the hydrolytic potential of CellicCTec2 promising to reduce the enzyme load and cost required for obtaining sugars from biorefinery relevant pre-treated substrates. |
