Keyword search (4,163 papers available)

"Yeast" Keyword-tagged Publications:

Title Authors PubMed ID
1 Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield Narcross L; Pyne ME; Kevvai K; Siu KH; Dueber JE; Martin VJJ; 41779670
BIOLOGY
2 PARPAL: PARalog Protein Redistribution using Abundance and Localization in Yeast Database Greco BM; Zapata G; Dandage R; Papkov M; Pereira V; Lefebvre F; Bourque G; Parts L; Kuzmin E; 40580499
BIOLOGY
3 A Humanized Yeast Model for Studying TRAPP Complex Mutations; Proof-of-Concept Using Variants from an Individual with a TRAPPC1-Associated Neurodevelopmental Syndrome Zykaj E; Abboud C; Asadi P; Warsame S; Almousa H; Milev MP; Greco BM; López-Sánchez M; Bratkovic D; Kachroo AH; Pérez-Jurado LA; Sacher M; 39273027
BIOLOGY
4 Genome sequencing of 15 acid-tolerant yeasts Bagley JA; Pyne ME; Exley K; Kevvai K; Wang Q; Whiteway M; Martin VJJ; 37747226
BIOLOGY
5 Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeast Sultana S; Abdullah M; Li J; Hochstrasser M; Kachroo AH; 37364278
BIOLOGY
6 Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeast Abdullah M; Greco BM; Laurent JM; Garge RK; Boutz DR; Vandeloo M; Marcotte EM; Kachroo AH; 37323580
BIOLOGY
7 Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera) Pyne ME; Gold ND; Martin VJJ; 37004909
BIOLOGY
8 The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit Bean BDM; Whiteway M; Martin VJJ; 35708612
BIOLOGY
9 Humanized yeast to model human biology, disease and evolution Kachroo AH; Vandeloo M; Greco BM; Abdullah M; 35661208
BIOLOGY
10 Discovery of new vascular disrupting agents based on evolutionarily conserved drug action, pesticide resistance mutations, and humanized yeast Garge RK; Cha HJ; Lee C; Gollihar JD; Kachroo AH; Wallingford JB; Marcotte EM; 34849907
BIOLOGY
11 Mechanisms that Link Chronological Aging to Cellular Quiescence in Budding Yeast. Mohammad K, Baratang Junio JA, Tafakori T, Orfanos E, Titorenko VI 32630624
BIOLOGY
12 SOD1 oxidation and formation of soluble aggregates in yeast: relevance to sporadic ALS development. Martins D, English AM 24936435
CHEMBIOCHEM
13 Lithocholic bile acid accumulated in yeast mitochondria orchestrates a development of an anti-aging cellular pattern by causing age-related changes in cellular proteome. Beach A, Richard VR, Bourque S, Boukh-Viner T, Kyryakov P, Gomez-Perez A, Arlia-Ciommo A, Feldman R, Leonov A, Piano A, Svistkova V, Titorenko VI 25839782
MASSSPEC
14 Caloric restriction extends yeast chronological lifespan via a mechanism linking cellular aging to cell cycle regulation, maintenance of a quiescent state, entry into a non-quiescent state and survival in the non-quiescent state. Leonov A, Feldman R, Piano A, Arlia-Ciommo A, Lutchman V, Ahmadi M, Elsaser S, Fakim H, Heshmati-Moghaddam M, Hussain A, Orfali S, Rajen H, Roofigari-Esfahani N, Rosanelli L, Titorenko VI 29050207
BIOLOGY
15 Some Metabolites Act as Second Messengers in Yeast Chronological Aging. Mohammad K, Dakik P, Medkour Y, McAuley M, Mitrofanova D, Titorenko VI 29543708
BIOLOGY
16 Caloric restriction delays yeast chronological aging by remodeling carbohydrate and lipid metabolism, altering peroxisomal and mitochondrial functionalities, and postponing the onsets of apoptotic and liponecrotic modes of regulated cell death. Arlia-Ciommo A, Leonov A, Beach A, Richard VR, Bourque SD, Burstein MT, Kyryakov P, Gomez-Perez A, Koupaki O, Feldman R, Titorenko VI 29662634
BIOLOGY
17 Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9. Akhmetov A, Laurent JM, Gollihar J, Gardner EC, Garge RK, Ellington AD, Kachroo AH, Marcotte EM 29770349
BIOLOGY
18 Mechanisms through which lithocholic acid delays yeast chronological aging under caloric restriction conditions. Arlia-Ciommo A, Leonov A, Mohammad K, Beach A, Richard VR, Bourque SD, Burstein MT, Goldberg AA, Kyryakov P, Gomez-Perez A, Koupaki O, Titorenko VI 30405886
BIOLOGY
19 Pairwise combinations of chemical compounds that delay yeast chronological aging through different signaling pathways display synergistic effects on the extent of aging delay. Dakik P, McAuley M, Chancharoen M, Mitrofanova D, Lozano Rodriguez ME, Baratang Junio JA, Lutchman V, Cortes B, Simard É, Titorenko VI 30719227
BIOLOGY
20 The evolutionary rewiring of the ribosomal protein transcription pathway modifies the interaction of transcription factor heteromer Ifh1-Fhl1 (interacts with forkhead 1-forkhead-like 1) with the DNA-binding specificity element. Mallick J, Whiteway M 23625919
BIOLOGY
21 Deconstructing the genetic basis of spent sulphite liquor tolerance using deep sequencing of genome-shuffled yeast. Pinel D, Colatriano D, Jiang H, Lee H, Martin VJ 25866561
CSFG
22 Reconstituting Plant Secondary Metabolism in Saccharomyces cerevisiae for Production of High-Value Benzylisoquinoline Alkaloids. Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ 27417930
CSFG
23 Determinants of selection in yeast evolved by genome shuffling. Biot-Pelletier D, Pinel D, Larue K, Martin VJJ 30356826
CSFG

 

Title:The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit
Authors:Bean BDMWhiteway MMartin VJJ
Link:https://pubmed.ncbi.nlm.nih.gov/35708612/
DOI:10.1093/g3journal/jkac154
Publication:G3 (Bethesda, Md.)
Keywords:CRISPRCas9MyLOcolocalizationintegrationlocalizationmicroscopyoverexpressiontoolkityeast
PMID:35708612 Category: Date Added:2022-06-16
Dept Affiliation: BIOLOGY
1 Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC, H4B1R6, Canada.

Description:

The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful CRISPR-Cas9-based genome editing tools we constructed a Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 toolkit with three components: (i) a set of optimized S. pyogenes Cas9-guide RNA (gRNA) expression vectors with five selectable markers and the option to either pre-clone or co-transform the gRNAs; (ii) vectors for the one-step construction of integration cassettes expressing an untagged or GFP/RFP/HA-tagged gene of interest at one of three levels, supporting localization and overexpression studies; and (iii) integration cassettes containing moderately expressed GFP- or RFP-tagged compartmental markers for colocalization experiments. These components allow rapid, high efficiency genomic integrations and modifications with only transient selection for the Cas9 vector, resulting in markerless transformations. To demonstrate the ease of use, we applied our complete set of compartmental markers to co-label all target subcellular compartments with GFP and RFP. Thus, the MyLO toolkit packages CRISPR-Cas9 technology into a flexible, optimized bundle that allows the stable genomic integration of DNA with the ease of use approaching that of transforming plasmids.





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