PERFORM Publications

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Title		Authors	PubMed ID
1	Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora	Marinovíc M; Di Falco M; Aguilar Pontes MV; Gorzsás A; Tsang A; de Vries RP; Mäkelä MR; Hildén K;	35892327 CSFG
2	Predicted coronavirus Nsp5 protease cleavage sites in the human proteome	Scott BM; Lacasse V; Blom DG; Tonner PD; Blom NS;	35379171 ENCS
3	Combination of system biology and classical approaches for developing biorefinery relevant lignocellulolytic Rasamsonia emersonii strain	Raheja Y; Singh V; Kaur B; Basotra N; Di Falco M; Tsang A; Singh Chadha B;	35318142 CSFG
4	Penicillium subrubescens adapts its enzyme production to the composition of plant biomass.	Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP	32408196 CSFG
5	Lithocholic bile acid accumulated in yeast mitochondria orchestrates a development of an anti-aging cellular pattern by causing age-related changes in cellular proteome.	Beach A, Richard VR, Bourque S, Boukh-Viner T, Kyryakov P, Gomez-Perez A, Arlia-Ciommo A, Feldman R, Leonov A, Piano A, Svistkova V, Titorenko VI	25839782 MASSSPEC
6	Evaluation of secretome of highly efficient lignocellulolytic Penicillium sp. Dal 5 isolated from rhizosphere of conifers.	Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS	27341464 CSFG
7	Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry	Robert J Gruninger	28417377 CSFG
8	Mass Spectrometry-Based Proteomics	Marcos Rafael Di Falco	29876812 CSFG
9	The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum.	Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP	30797054 CSFG

Title:	Mass Spectrometry-Based Proteomics
Authors:	Marcos Rafael Di Falco
Link:	https://pubmed.ncbi.nlm.nih.gov/29876812/
DOI:	10.1007/978-1-4939-7804-5_9
Publication:	Methods in molecular biology (Clifton, N.J.)
Keywords:	Database search; Fungal proteome; In-gel digestion; In-solution digestion; LC-MS/MS; Microcapillary column packing;
PMID:	29876812	Category:	Methods Mol Biol	Date Added:	2019-06-07
Dept Affiliation:	CSFG 1 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada. marcos.difalco@concordia.ca.

Description:

Proteomics is the large-scale analysis of proteins rendered possible by modern mass spectrometry analysis methods capable of identifying thousands of peptides/proteins in a fast high-throughput manner. Here I describe protocols for the preparation of fungal culture protein samples for mass spectrometry-based proteomics analysis including protein sample cleanup, proteolytic digestion, LC-MS/MS separation, and database search protein identification.

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