Keyword search (4,163 papers available)

"PCR" Keyword-tagged Publications:

Title Authors PubMed ID
1 eDNA Provides Accurate Population Abundance Estimates With Bioenergetics and Particle Mass-Balance Modelling Beaulieu J; Yates MC; Fraser DJ; Cristescu ME; Derry AM; 41913704
BIOLOGY
2 Longitudinal Associations Between Relationship Quality and Depression Among Youth with Intellectual Disabilities: A Latent Change Perspective Dubé C; Morin AJS; Olivier E; Tóth-Király I; Tracey D; Craven RG; Maïano C; 36436145
PSYCHOLOGY
3 Tools and Techniques for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)/COVID-19 Detection Safiabadi Tali SH; LeBlanc JJ; Sadiq Z; Oyewunmi OD; Camargo C; Nikpour B; Armanfard N; Sagan SM; Jahanshahi-Anbuhi S; 33980687
IMAGING
4 The Biology of Vasopressin. Sparapani S, Millet-Boureima C, Oliver J, Mu K, Hadavi P, Kalostian T, Ali N, Avelar CM, Bardies M, Barrow B, Benedikt M, Biancardi G, Bindra R, Bui L, Chihab Z, Cossitt A, Costa J, Daigneault T, Dault J, Davidson I, Dias J, Dufour E, El-Khoury S, Farhangdoost N, Forget A, Fox A, Gebrael M, Gentile MC, Geraci O, Gnanapragasam A, Gomah E, Haber E, Hamel C, Iyanker T, Kalantzis C, Kamali S, Kassardjian E, Kontos HK, Le TBU, LoScerbo D, Low YF, Mac Rae D, Maurer F, Mazhar S, Nguyen A, Nguyen-Duong K, Osborne-L 33477721
BIOLOGY
5 Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing. Bellemare A, John T, Marqueteau S 29876805
CSFG

 

Title:Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing.
Authors:Bellemare A,  John T,  Marqueteau S
Link:https://www.ncbi.nlm.nih.gov/pubmed/29876805?dopt=Abstract
DOI:10.1007/978-1-4939-7804-5_2
Publication:Methods in molecular biology (Clifton, N.J.)
Keywords:Agarose gel electrophoresis;  Colony PCR;  DNA quantification;  Fluorometry;  Fungal genomic DNA extraction;  Mycelia;  Spectrophotometer; 
PMID:29876805 Category:Methods Mol Biol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada. annie.bellemare@concordia.ca.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada.

Description:

Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing.

Methods Mol Biol. 2018;1775:11-20

Authors: Bellemare A, John T, Marqueteau S

Abstract

Isolation of fungal genomic DNA of high quality is required for a number of downstream biotechnology-derived applications such as genome sequencing, microarrays, and digital PCR technologies, to only name a few. In most cases, not only a high molecular weight DNA of superior grade is required but also large quantities. On the other hand, a number of laboratory experiments, such as polymerase chain reaction (PCR) for medical diagnostic or for genotyping, have to be conducted in a limited amount of time and can provide complete results with the use of lower quality DNA. We describe here two different fungal DNA extraction approaches, which are applicable to a wide range of fungal species.First, we adapted a DNA extraction method for PCR-based genotyping which allows analysis of single to hundreds of colonies simultaneously. Cells are disrupted in the presence of sodium dodecyl sulfate and Proteinase K which are then removed by precipitation and centrifugation. The cleared lysate is used for PCR reaction.Secondly, we describe a method to obtain genome sequencing quality grade DNA from fungal liquid cultures. Mycelia are harvested by either filtration or centrifugation. Cells are mechanically disrupted by liquid nitrogen grinding, followed by genomic DNA extraction using the QIAGEN's DNeasy® Plant Kit. The quality and quantity of genomic DNA is monitored by fluorometry.

PMID: 29876805 [PubMed - indexed for MEDLINE]




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