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PubMed Publications| Keyword search (4,163 papers available) |  |
"Mycothermus" Keyword-tagged Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Combination of system biology and classical approaches for developing biorefinery relevant lignocellulolytic Rasamsonia emersonii strain | Raheja Y; Singh V; Kaur B; Basotra N; Di Falco M; Tsang A; Singh Chadha B; | 35318142 CSFG |
| 2 | Mycothermus thermophilus gen. et comb. nov., a new home for the itinerant thermophile Scytalidium thermophilum (Torula thermophila). | Natvig DO, Taylor JW, Tsang A, Hutchinson MI, Powell AJ | 25550298 CSFG |
| 3 | Mycothermus thermophilus (Syn. Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed | Neha Basotra | 27744242 CSFG |
| Title: | Combination of system biology and classical approaches for developing biorefinery relevant lignocellulolytic Rasamsonia emersonii strain | ||||
| Authors: | Raheja Y, Singh V, Kaur B, Basotra N, Di Falco M, Tsang A, Singh Chadha B | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/35318142/ | ||||
| DOI: | 10.1016/j.biortech.2022.127039 | ||||
| Publication: | Bioresource technology | ||||
| Keywords: | Hydrolysis; Mycothermus thermophiloides; Proteome; Rasamsonia emersonii; Transcriptional level; | ||||
| PMID: | 35318142 | Category: | Date Added: | 2022-03-23 | |
| Dept Affiliation: |
CSFG
1 Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. 2 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec H4B 1R6, Canada. 3 Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: chadhabs@yahoo.com. |
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Description: |
The objective of this study was to develop thermophilic fungus Rasamsonia emersonii using integrated system biology tools (genomics, proteomics and transcriptional analysis) in combination with classical strain breeding approaches. Developed hyper cellulolytic mutant strain M36 showed endoglucanase (476.35 U/ml), ß-glucosidase (70.54 U/ml), cellobiohydrolase (15.17 U/ml), FPase (4.89 U/ml) and xylanase (485.21 U/ml) on cellulose/gram flour based production medium. Comparison of the expression profile at proteome and transcriptional level of the developed strain and wild type parent gave detailed insight into the up-regulation of different CAZymes including glycosyl hydrolases (GH5, GH6, GH7, GH3, GH10) and auxiliary enzymes (lytic polysaccharide monooxygenase, swollenin) at system level. Furthermore, the potential of lignocellulolytic enzyme produced by the developed strain and custom designed cocktail spiked with heterologously expressed lytic polysaccharide monooxygenase from Mycothermus thermophiloides were analyzed for the hydrolysis of biorefinery relevant unwashed pretreated rice straw slurry (PRAJ and IOCL) @17% substrate loading rate. |
