Keyword search (4,163 papers available)

"Mutagenesis" Keyword-tagged Publications:

Title Authors PubMed ID
1 The enterobactin biosynthetic intermediate 2,3-dihydroxybenzoic acid is a competitive inhibitor of the Escherichia coli isochorismatase EntB Bin X; Pawelek PD; 40400396
CHEMBIOCHEM
2 Developing endophytic Penicillium oxalicum as a source of lignocellulolytic enzymes for enhanced hydrolysis of biorefinery relevant pretreated rice straw Sharma G; Kaur B; Raheja Y; Kaur A; Singh V; Basotra N; Di Falco M; Tsang A; Chadha BS; 39249151
CSFG
3 Evidence of isochorismate channeling between the Escherichia coli enterobactin biosynthetic enzymes EntC and EntB Bin X; Pawelek PD; 39031458
CHEMBIOCHEM
4 Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance. Lubbers RJM, Liwanag AJ, Peng M, Dilokpimol A, Benoit-Gelber I, de Vries RP 31674709
CSFG
5 Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9. Biot-Pelletier D, Martin VJ 27134651
BIOLOGY

 

Title:Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9.
Authors:Biot-Pelletier D,  Martin VJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/27134651?dopt=Abstract
DOI:10.1186/s13036-016-0028-1
Publication:Journal of biological engineering
Keywords:CRISPR-Cas9;  Genome editing;  Saccharomyces cerevisiae;  Site-directed mutagenesis; 
PMID:27134651 Category:J Biol Eng Date Added:2019-06-07
Dept Affiliation: BIOLOGY
1 Department of Biology, Concordia University, 7141 Sherbrooke West, Montréal, QC H4B 1R6 Canada ; Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke West, Montréal, QC H4B 1R6 Canada.

Description:

Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9.

J Biol Eng. 2016;10:6

Authors: Biot-Pelletier D, Martin VJ

Abstract

CRISPR assisted homology directed repair enables the introduction of virtually any modification to the Saccharomyces cerevisiae genome. Of obvious interest is the marker-free and seamless introduction of point mutations. To fulfill this promise, a strategy that effects single nucleotide changes while preventing repeated recognition and cutting by the gRNA/Cas9 complex is needed. We demonstrate a two-step method to introduce point mutations at 17 positions in the S. cerevisiae genome. We show the general applicability of the method, enabling the seamless introduction of single nucleotide changes at any location, including essential genes and non-coding regions. We also show a quantifiable phenotype for a point mutation introduced in gene GSH1. The ease and wide applicability of this general method, combined with the demonstration of its feasibility will enable genome editing at an unprecedented level of detail in yeast and other organisms.

PMID: 27134651 [PubMed]




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