Keyword search (4,163 papers available)

"Membrane fusion" Keyword-tagged Publications:

Title Authors PubMed ID
1 Distinct features of multivesicular body-lysosome fusion revealed by a new cell-free content-mixing assay. Karim MA, Samyn DR, Mattie S, Brett CL 29135058
BIOLOGY
2 Rab-Effector-Kinase Interplay Modulates Intralumenal Fragment Formation during Vacuole Fusion. Karim MA, McNally EK, Samyn DR, Mattie S, Brett CL 30269949
BIOLOGY
3 A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion. Karim MA, Samyn DR, Brett CL 30317513
BIOLOGY
4 Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy. Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL 30317518
BIOLOGY

 

Title:Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.
Authors:Mattie S,  Kazmirchuk T,  Mui J,  Vali H,  Brett CL
Link:https://www.ncbi.nlm.nih.gov/pubmed/30317518?dopt=Abstract
DOI:10.1007/978-1-4939-8760-3_24
Publication:Methods in molecular biology (Clifton, N.J.)
Keywords:Cryogenic electron microscopy (cryoEM);  Hemifusion;  Lipid bilayer merger;  Lysosome;  Membrane fusion;  SNARE;  Tomography;  Transmission electron microscopy (TEM);  Vacuole; 
PMID:30317518 Category:Methods Mol Biol Date Added:2019-06-07
Dept Affiliation: BIOLOGY
1 Department of Biology, Concordia University, Montréal, QC, Canada.
2 Montreal Neurological Hospital and Institute, McGill University, Montréal, QC, Canada.
3 Facility for Electron Microscopy Research, Department of Anatomy and Cell Biology, McGill University, Montréal, QC, Canada.
4 Department of Biology, Concordia University, Montréal, QC, Canada. christopher.brett@concordia.ca.

Description:

Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.

Methods Mol Biol. 2019;1860:361-377

Authors: Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL

Abstract

SNARE-mediated membrane fusion is required for membrane trafficking as well as organelle biogenesis and homeostasis. The membrane fusion reaction involves sequential formation of hemifusion intermediates, whereby lipid monolayers partially mix on route to complete bilayer merger. Studies of the Saccharomyces cerevisiae lysosomal vacuole have revealed many of the fundamental mechanisms that drive the membrane fusion process, as well as features unique to organelle fusion. However, until recently, it has not been amenable to electron microscopy methods that have been invaluable for studying hemifusion in other model systems. Herein, we describe a method to visualize hemifusion intermediates during homotypic vacuole membrane fusion in vitro by transmission electron microscopy (TEM), electron tomography, and cryogenic electron microscopy (cryoEM). This method facilitates acquisition of invaluable ultrastructural data needed to comprehensively understand how fusogenic lipids and proteins contribute to SNARE-mediated membrane fusion-by-hemifusion and the unique features of organelle versus small-vesicle fusion.

PMID: 30317518 [PubMed - indexed for MEDLINE]




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