Keyword search (4,163 papers available)

"Fungi" Keyword-tagged Publications:

Title Authors PubMed ID
1 Season and city shape urban bioaerosol composition beyond vegetation and socioeconomic gradients Poirier S; Rondeau-Leclaire J; Faticov M; Roy A; Lajeunesse G; Lucier JF; Tardif S; Kembel SW; Ziter C; Laprise C; Paquette A; Girard C; Laforest-Lapointe I; 41785576
BIOLOGY
2 Contrasting microbial assembly patterns in the woody endosphere of hybrid and non-hybrid em Populus /em trees Grant KR; Kembel SW; Naik S; Dayanandan S; 41089252
BIOLOGY
3 The Bug-Network (BugNet): A Global Experimental Network Testing the Effects of Invertebrate Herbivores and Fungal Pathogens on Plant Communities and Ecosystem Function in Open Ecosystems Kempel A; Adamidis GC; Anadón JD; Atkinson J; Auge H; Avtzis D; Bachelot B; Bashirzadeh M; Bota JL; Classen A; Constantinou I; Crawley M; de Bellis T; Dostal P; Ebeling A; Eisenhauer N; Eldridge DJ; Encina G; Estrada C; Everingham S; Fanin N; Feng Y; Gaspar M; Gooriah L; Graff P; Montalván EG; Montalván PG; Hartke TR; Huang L; Jochum M; Kaljund K; Karmiris I; Koorem K; Korell L; Laine AL; le Provost G; Lessard JP; Liu M; Liu X; Liu Y; Llancabure J; Loïez S; Loydi A; Marrero H; Gockel S; Montoya A; Münzbergo 41080499
ENCS
4 An examination of the quinic acid utilization genes in Aspergillus niger reveals the involvement of two pH-dependent permeases Sgro M; Reid ID; Arentshorst M; Ram AFJ; Tsang A; 40853219
GENOMICS
5 The temperate forest phyllosphere and rhizosphere microbiome: a case study of sugar maple Enea M; Beauregard J; De Bellis T; Faticov M; Laforest-Lapointe I; 39881993
BIOLOGY
6 Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger Sgro M; Chow N; Olyaei F; Arentshorst M; Geoffrion N; Ram AFJ; Powlowski J; Tsang A; 37399977
BIOLOGY
7 Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics Gruninger RJ; Tsang A; McAllister TA; 37149538
CSFG
8 Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora Marinovíc M; Di Falco M; Aguilar Pontes MV; Gorzsás A; Tsang A; de Vries RP; Mäkelä MR; Hildén K; 35892327
CSFG
9 Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases Dilokpimol A; Verkerk B; Li X; Bellemare A; Lavallee M; Frommhagen M; Nørmølle Underlin E; Kabel MA; Powlowski J; Tsang A; de Vries RP; 35187647
CSFG
10 The Canadian Fungal Research Network: current challenges and future opportunities. Horianopoulos LC, Gluck-Thaler E, Benoit Gelber I, Cowen LE, Geddes-McAlister J, Landry CR, Schwartz IS, Scott JA, Sellam A, Sheppard DC, Spribille T, Subramaniam R, Walker AK, Harris SD, Shapiro RS, Gerstein A 32717148
BIOLOGY
11 Discovery and Expression of Thermostable LPMOs from Thermophilic Fungi for Producing Efficient Lignocellulolytic Enzyme Cocktails. Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS 31792786
CSFG
12 Shared mycorrhizae but distinct communities of other root-associated microbes on co-occurring native and invasive maples. DeBellis T, Kembel SW, Lessard JP 31392089
BIOLOGY
13 Enzymes of early-diverging, zoosporic fungi. Lange L, Barrett K, Pilgaard B, Gleason F, Tsang A 31309267
CSFG
14 Mycothermus thermophilus gen. et comb. nov., a new home for the itinerant thermophile Scytalidium thermophilum (Torula thermophila). Natvig DO, Taylor JW, Tsang A, Hutchinson MI, Powell AJ 25550298
CSFG
15 Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics. Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA 28417376
CSFG
16 Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry Robert J Gruninger 28417377
CSFG
17 Discovery and characterization of family 39 glycoside hydrolases from rumen anaerobic fungi with polyspecific activity on rare arabinosyl substrates. Jones DR, Uddin MS, Gruninger RJ, Pham TTM, Thomas D, Boraston AB, Briggs J, Pluvinage B, McAllister TA, Forster RJ, Tsang A, Selinger LB, Abbott DW 28588026
CSFG
18 Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi. Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T 29803771
CSFG
19 Introduction: Overview of Fungal Genomics. de Vries RP, Grigoriev IV, Tsang A 29876804
CSFG
20 Application of Transcriptomics to Compare the Carbohydrate Active Enzymes That Are Expressed by Diverse Genera of Anaerobic Fungi to Degrade Plant Cell Wall Carbohydrates. Gruninger RJ, Nguyen TTM, Reid ID, Yanke JL, Wang P, Abbott DW, Tsang A, McAllister T 30061875
CSFG
21 Thermostable xylanases from thermophilic fungi and bacteria: Current perspective. Chadha BS, Kaur B, Basotra N, Tsang A, Pandey A 30679061
CSFG

 

Title:Discovery and Expression of Thermostable LPMOs from Thermophilic Fungi for Producing Efficient Lignocellulolytic Enzyme Cocktails.
Authors:Agrawal DBasotra NBalan VTsang AChadha BS
Link:https://www.ncbi.nlm.nih.gov/pubmed/31792786?dopt=Abstract
DOI:10.1007/s12010-019-03198-5
Publication:Applied biochemistry and biotechnology
Keywords:H2O2Heterologous expressionHydrolysisLytic polysaccharide monooxygenases (LPMOs)Thermophilic fungi
PMID:31792786 Category:Appl Biochem Biotechnol Date Added:2019-12-04
Dept Affiliation: CSFG
1 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.
2 Department Engineering Technology, Biotechnology Program, College of Technology, University of Houston, Houston, TX, 77004, USA.
3 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec, H4B 1R6, Canada.
4 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. chadhabs@yahoo.com.

Description:

Discovery and Expression of Thermostable LPMOs from Thermophilic Fungi for Producing Efficient Lignocellulolytic Enzyme Cocktails.

Appl Biochem Biotechnol. 2019 Dec 02;:

Authors: Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS

Abstract

In this study, two novel thermostable lytic polysaccharide monooxygenases (LPMOs) were cloned from thermophilic fungus Scytalidium thermophilum (PMO9D_SCYTH) and Malbranchea cinnamomea (PMO9D_MALCI) and expressed in the methylotrophic yeast Pichia pastoris X33. The purified PMO9D_SCYTH was active at 60 °C (t1/2 = 60.58 h, pH 7.0), whereas, PMO9D_MALCI was optimally active at 50 °C (t1/2 = 144 h, pH 7.0). The respective catalytic efficiency (kcat/Km) of PMO9D_SCYTH and PMO9D_MALCI determined against avicel in presence of H2O2 was (6.58 × 10-3 and 1.79 × 10-3 mg-1 ml min-1) and carboxy-methylcellulose (CMC) (1.52 × 10-1 and 2.62 × 10-2 mg-1 ml min-1). The HRMS analysis of products obtained after hydrolysis of avicel and CMC showed the presence of both C1 and C4 oxidized oligosaccharides, in addition to phylogenetic tree constructed with other characterized type 1 and 3 LPMOs demonstrated that both LPMOs belongs to type-3 family of AA9s. The release of sugars during saccharification of acid/alkali pretreated sugarcane bagasse and rice straw was enhanced upon replacing one part of commercial enzyme Cellic CTec2 with these LPMOs.

PMID: 31792786 [PubMed - as supplied by publisher]





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