PERFORM Publications

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Title		Authors	PubMed ID
1	Effects of chronodisruption and alcohol consumption on gene expression in reward-related brain areas in female rats	Meyer C; Schoettner K; Amir S;	39624490 PSYCHOLOGY
2	Transcriptomics identify the triggering of citrate export as the key event caused by manganese deficiency in Aspergillus niger	Fekete E; Bíró V; Márton A; Bakondi-Kovács I; Sándor E; Kovács B; Geoffrion N; Tsang A; Kubicek CP; Karaffa L;	39377610 CSFG
3	Prototype Facial Response to Cute Stimuli: Expression and Recognition	O' Neil MJ; Danvers AF; Hu JI; Shiota MN;	39282978 CONCORDIA
4	Acute ethanol disrupts conditioned inhibition in the male rat	Germé K; Pfaus JG;	38822097 CSBN
5	A thermostable and inhibitor resistant β-glucosidase from Rasamsonia emersonii for efficient hydrolysis of lignocellulosics biomass	Raheja Y; Singh V; Sharma G; Tsang A; Chadha BS;	38470501 CSFG
6	CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii	Singh V; Raheja Y; Basotra N; Sharma G; Tsang A; Chadha BS;	37658430 CSFG
7	The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit	Bean BDM; Whiteway M; Martin VJJ;	35708612 BIOLOGY
8	Characterization of the heterotrimeric G protein gene families in Triticum aestivum and related species	Gawande ND; Hamiditabar Z; Brunetti SC; Gulick PJ;	35463045 BIOLOGY
9	ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance	Lismer A; Lambrot R; Lafleur C; Dumeaux V; Kimmins S;	34159325 PERFORM
10	Discovery and Expression of Thermostable LPMOs from Thermophilic Fungi for Producing Efficient Lignocellulolytic Enzyme Cocktails.	Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS	31792786 CSFG
11	Proteomic Analysis of Morphologically Changed Tissues after Prolonged Dexamethasone Treatment	Malkawi AK; Masood A; Shinwari Z; Jacob M; Benabdelkamel H; Matic G; Almuhanna F; Dasouki M; Alaiya AA; Rahman AMA;	31247941 CHEMBIOCHEM
12	Characterization of the Esi3/RCI2/PMP3 gene family in the Triticeae.	Brunetti SC, Arseneault MKM, Gulick PJ	30537926 BIOLOGY
13	The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor.	Pereira EO, Tsang A, McAllister TA, Menassa R	23915965 CSFG
14	Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics.	Basotra N, Joshi S, Satyanarayana T, Pati PK, Tsang A, Chadha BS	29174359 CSFG

Title:	The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor.
Authors:	Pereira EO, Tsang A, McAllister TA, Menassa R
Link:	https://www.ncbi.nlm.nih.gov/pubmed/23915965?dopt=Abstract
DOI:	10.1186/1754-6834-6-111
Publication:	Biotechnology for biofuels
Keywords:	Acetylxylan esterase Acremonium alcalophilum; Heterologous expression; Lipase; Nicotiana benthamiana;
PMID:	23915965	Category:	Biotechnol Biofuels	Date Added:	2019-06-07
Dept Affiliation:	CSFG 1 Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada ; Department of Biology, The University of Western Ontario, London, ON N6A 5B7, Canada. 2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec H4B 1R6, Canada. 3 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB T1J 4B1, Canada.

Description:

The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor.

Biotechnol Biofuels. 2013;6:111

Authors: Pereira EO, Tsang A, McAllister TA, Menassa R

Abstract

BACKGROUND: Microorganisms are the most proficient decomposers in nature, using secreted enzymes in the hydrolysis of lignocellulose. As such, they present the most abundant source for discovery of new enzymes. Acremonium alcalophilum is the only known cellulolytic fungus that thrives in alkaline conditions and can be cultured readily in the laboratory. Its optimal conditions for growth are 30°C and pH 9.0-9.2. The genome sequence of Acremonium alcalophilum has revealed a large number of genes encoding biomass-degrading enzymes. Among these enzymes, lipases are interesting because of several industrial applications including biofuels, detergent, food processing and textile industries.

RESULTS: We identified a lipA gene in the genome sequence of Acremonium alcalophilum, encoding a protein with a predicted lipase domain with weak sequence identity to characterized enzymes. Unusually, the predicted lipase displays?˜?30% amino acid sequence identity to both feruloyl esterase and lipase of Aspergillus niger. LipA, when transiently produced in Nicotiana benthamiana, accumulated to over 9% of total soluble protein. Plant-produced recombinant LipA is active towards p-nitrophenol esters of various carbon chain lengths with peak activity on medium-chain fatty acid (C8). The enzyme is also highly active on xylose tetra-acetate and oat spelt xylan. These results suggests that LipA is a novel lipolytic enzyme that possesses both lipase and acetylxylan esterase activity. We determined that LipA is a glycoprotein with pH and temperature optima at 8.0 and 40°C, respectively.

CONCLUSION: Besides being the first heterologous expression and characterization of a gene coding for a lipase from A. alcalophilum, this report shows that LipA is very versatile exhibiting both acetylxylan esterase and lipase activities potentially useful for diverse industry sectors, and that tobacco is a suitable bioreactor for producing fungal proteins.

PMID: 23915965 [PubMed]

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