Keyword search (4,163 papers available)

"Enzymes" Keyword-tagged Publications:

Title Authors PubMed ID
1 Functional screening pipeline to uncover laccase-like multicopper oxidase enzymes that transform industrial lignins Sharan AA; Bellemare A; DiFalco M; Tsang A; Vuong TV; Edwards EA; Master ER; 38000639
CSFG
2 Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization in Aspergillus niger Arentshorst M; Falco MD; Moisan MC; Reid ID; Spaapen TOM; van Dam J; Demirci E; Powlowski J; Punt PJ; Tsang A; Ram AFJ; 37744122
CSFG
3 Evidence for ligninolytic activity of the ascomycete fungus Podospora anserina. van Erven G, Kleijn AF, Patyshakuliyeva A, Di Falco M, Tsang A, de Vries RP, van Berkel WJH, Kabel MA 32322305
CSFG
4 Effect and ameliorative mechanisms of polyoxometalates on the denitrification under sulfonamide antibiotics stress. Guo H, Chen Z, Lu C, Guo J, Li H, Song Y, Han Y, Hou Y 32145698
ENCS
5 Enzymes of early-diverging, zoosporic fungi. Lange L, Barrett K, Pilgaard B, Gleason F, Tsang A 31309267
CSFG
6 Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila. Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A 24881579
BIOLOGY
7 Identification of novel enzymes to enhance the ruminal digestion of barley straw Badhan A; Ribeiro GO; Jones DR; Wang Y; Abbott DW; Di Falco M; Tsang A; McAllister TA; 29621684
CSFG
8 Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi. Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T 29803771
CSFG
9 New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw. Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA 30053012
CSFG
10 Application of Transcriptomics to Compare the Carbohydrate Active Enzymes That Are Expressed by Diverse Genera of Anaerobic Fungi to Degrade Plant Cell Wall Carbohydrates. Gruninger RJ, Nguyen TTM, Reid ID, Yanke JL, Wang P, Abbott DW, Tsang A, McAllister T 30061875
CSFG
11 The obligate alkalophilic soda-lake fungus Sodiomyces alkalinus has shifted to a protein diet. Grum-Grzhimaylo AA, Falkoski DL, van den Heuvel J, Valero-Jiménez CA, Min B, Choi IG, Lipzen A, Daum CG, Aanen DK, Tsang A, Henrissat B, Bilanenko EN, de Vries RP, van Kan JAL, Grigoriev IV, Debets AJM 30368956
CSFG

 

Title:Identification of novel enzymes to enhance the ruminal digestion of barley straw
Authors:Badhan ARibeiro GOJones DRWang YAbbott DWDi Falco MTsang AMcAllister TA
Link:https://pubmed.ncbi.nlm.nih.gov/29621684/
DOI:10.1016/j.biortech.2018.03.086
Publication:Bioresource technology
Keywords:Blue Native PAGECarbohydrate active enzymes (CAZymes)Fiber utilizationGlycosyl hydrolaseLC-MS/MSRumen nutrition
PMID:29621684 Category:Bioresour Technol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec H4B 1R6, Canada.
3 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada. Electronic address: tim.mcallister@agr.gc.ca.

Description:

Crude enzyme extracts typically contain a broad spectrum of enzyme activities, most of which are redundant to those naturally produced by the rumen microbiome. Identification of enzyme activities that are synergistic to those produced by the rumen microbiome could enable formulation of enzyme cocktails that improve fiber digestion in ruminants. Compared to untreated barley straw, Viscozyme® increased gas production, dry matter digestion (P < 0.01) and volatile fatty acid production (P < 0.001) in ruminal batch cultures. Fractionation of Viscozyme® by Blue Native PAGE and analyses using a microassay and mass-spectrometry revealed a GH74 endoglucanase, GH71 a-1,3-glucanase, GH5 mannanase, GH7 cellobiohydrolase, GH28 pectinase, and esterases from Viscozyme® contributed to enhanced saccharification of barley straw by rumen mix enzymes. Grouping of these identified activities with their carbohydrate active enzymes (CAZy) counterparts enabled selection of similar CAZymes for downstream production and screening. Mining of these specific activities from other biological systems could lead to high value enzyme formulations for ruminants.





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