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Title Authors PubMed ID
1 Mass Spectrometry-Based Proteomics Marcos Rafael Di Falco 29876812
CSFG

 

Title:Mass Spectrometry-Based Proteomics
Authors:Marcos Rafael Di Falco
Link:https://pubmed.ncbi.nlm.nih.gov/29876812/
DOI:10.1007/978-1-4939-7804-5_9
Publication:Methods in molecular biology (Clifton, N.J.)
Keywords:Database searchFungal proteomeIn-gel digestionIn-solution digestionLC-MS/MSMicrocapillary column packing
PMID:29876812 Category:Methods Mol Biol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada. marcos.difalco@concordia.ca.

Description:

Proteomics is the large-scale analysis of proteins rendered possible by modern mass spectrometry analysis methods capable of identifying thousands of peptides/proteins in a fast high-throughput manner. Here I describe protocols for the preparation of fungal culture protein samples for mass spectrometry-based proteomics analysis including protein sample cleanup, proteolytic digestion, LC-MS/MS separation, and database search protein identification.





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