Keyword search (4,163 papers available)

"Cas9" Keyword-tagged Publications:

Title Authors PubMed ID
1 CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii Singh V; Raheja Y; Basotra N; Sharma G; Tsang A; Chadha BS; 37658430
CSFG
2 CRAPS: Chromosomal-Repair-Assisted Pathway Shuffling in Yeast Dykstra CB; Pyne ME; Martin VJJ; 37584634
BIOLOGY
3 Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeast Abdullah M; Greco BM; Laurent JM; Garge RK; Boutz DR; Vandeloo M; Marcotte EM; Kachroo AH; 37323580
BIOLOGY
4 The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit Bean BDM; Whiteway M; Martin VJJ; 35708612
BIOLOGY
5 The chimeric GaaR-XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger Kun RS; Garrigues S; Di Falco M; Tsang A; de Vries RP; 34236481
CSFG
6 Identification of a Novel Biosynthetic Gene Cluster in Aspergillus niger Using Comparative Genomics Evdokias G; Semper C; Mora-Ochomogo M; Di Falco M; Nguyen TTM; Savchenko A; Tsang A; Benoit-Gelber I; 34064722
BIOLOGY
7 Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9. Biot-Pelletier D, Martin VJ 27134651
BIOLOGY
8 W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger. Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ 30298571
CSFG

 

Title:W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.
Authors:Alazi E,  Niu J,  Otto SB,  Arentshorst M,  Pham TTM,  Tsang A,  Ram AFJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/30298571?dopt=Abstract
DOI:10.1002/mbo3.732
Publication:MicrobiologyOpen
Keywords:Aspergillus niger;  CRISPR-Cas9;  constitutively active transcription factor;  missense mutation;  pectinase;  transcription factor localization; 
PMID:30298571 Category:Microbiologyopen Date Added:2019-06-07
Dept Affiliation: CSFG
1 Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Québec, Canada.

Description:

W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.

Microbiologyopen. 2019 May;8(5):e00732

Authors: Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ

Abstract

Polysaccharides present in plant biomass, such as pectin, are the main carbon source for filamentous fungi. Aspergillus niger naturally secretes pectinases to degrade pectin and utilize the released monomers, mainly D-galacturonic acid. The transcriptional activator GaaR, the repressor of D-galacturonic acid utilization GaaX, and the physiological inducer 2-keto-3-deoxy-L-galactonate play important roles in the transcriptional regulation of D-galacturonic acid-responsive genes, which include the genes encoding pectinases. In this study, we described the mutations found in gaaX and gaaR that enabled constitutive (i.e., inducer-independent) expression of pectinases by A. niger. Using promoter-reporter strains (PpgaX-amdS) and polygalacturonic acid plate assays, we showed that W361R mutation in GaaR results in constitutive production of pectinases. Analysis of subcellular localization of C-terminally eGFP-tagged GaaR/GaaRW 361R revealed important differences in nuclear accumulation of N- versus C-terminally eGFP-tagged GaaR.

PMID: 30298571 [PubMed - in process]




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