Keyword search (4,163 papers available)

"CRISPR" Keyword-tagged Publications:

Title Authors PubMed ID
1 Tri-Functional CRISPR Screen Reveals Overexpression of em QDR2 /em and em QDR3 /em Transporters Increase Fumaric Acid Production in em Kluyveromyces marxianus /em Thornbury M; Omran RP; Kumar L; Knoops A; Abushahin R; Whiteway M; Martin VJJ; 41277095
BIOLOGY
2 Sequencing of a Dairy Isolate Unlocks em Kluyveromyces marxianus /em as a Host for Lactose Valorization Thornbury M; Knoops A; Summerby-Murray I; Dhaliwal J; Johnson S; Utomo JC; Joshi J; Narcross L; Remondetto G; Pouliot M; Whiteway M; Martin VJJ; 40629255
BIOLOGY
3 Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies Husser MC; Pham NP; Law C; Araujo FRB; Martin VJJ; Piekny A; 38652106
BIOLOGY
4 CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii Singh V; Raheja Y; Basotra N; Sharma G; Tsang A; Chadha BS; 37658430
CSFG
5 CRAPS: Chromosomal-Repair-Assisted Pathway Shuffling in Yeast Dykstra CB; Pyne ME; Martin VJJ; 37584634
BIOLOGY
6 Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeast Abdullah M; Greco BM; Laurent JM; Garge RK; Boutz DR; Vandeloo M; Marcotte EM; Kachroo AH; 37323580
BIOLOGY
7 Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ; 36416720
BIOLOGY
8 The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit Bean BDM; Whiteway M; Martin VJJ; 35708612
BIOLOGY
9 The chimeric GaaR-XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger Kun RS; Garrigues S; Di Falco M; Tsang A; de Vries RP; 34236481
CSFG
10 Identification of a Novel Biosynthetic Gene Cluster in Aspergillus niger Using Comparative Genomics Evdokias G; Semper C; Mora-Ochomogo M; Di Falco M; Nguyen TTM; Savchenko A; Tsang A; Benoit-Gelber I; 34064722
BIOLOGY
11 Using the endogenous CRISPR-Cas system of Heliobacterium modesticaldum to delete the photochemical reaction center core subunit gene. Baker PL, Orf GS, Kevershan K, Pyne ME, Bicer T, Redding KE 31540988
BIOLOGY
12 Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9. Akhmetov A, Laurent JM, Gollihar J, Gardner EC, Garge RK, Ellington AD, Kachroo AH, Marcotte EM 29770349
BIOLOGY
13 A Highly Characterized Synthetic Landing Pad System for Precise Multicopy Gene Integration in Yeast. Bourgeois L, Pyne ME, Martin VJJ 30372609
BIOLOGY
14 Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9. Biot-Pelletier D, Martin VJ 27134651
BIOLOGY
15 W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger. Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ 30298571
CSFG

 

Title:W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.
Authors:Alazi E,  Niu J,  Otto SB,  Arentshorst M,  Pham TTM,  Tsang A,  Ram AFJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/30298571?dopt=Abstract
DOI:10.1002/mbo3.732
Publication:MicrobiologyOpen
Keywords:Aspergillus niger;  CRISPR-Cas9;  constitutively active transcription factor;  missense mutation;  pectinase;  transcription factor localization; 
PMID:30298571 Category:Microbiologyopen Date Added:2019-06-07
Dept Affiliation: CSFG
1 Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Québec, Canada.

Description:

W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.

Microbiologyopen. 2019 May;8(5):e00732

Authors: Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ

Abstract

Polysaccharides present in plant biomass, such as pectin, are the main carbon source for filamentous fungi. Aspergillus niger naturally secretes pectinases to degrade pectin and utilize the released monomers, mainly D-galacturonic acid. The transcriptional activator GaaR, the repressor of D-galacturonic acid utilization GaaX, and the physiological inducer 2-keto-3-deoxy-L-galactonate play important roles in the transcriptional regulation of D-galacturonic acid-responsive genes, which include the genes encoding pectinases. In this study, we described the mutations found in gaaX and gaaR that enabled constitutive (i.e., inducer-independent) expression of pectinases by A. niger. Using promoter-reporter strains (PpgaX-amdS) and polygalacturonic acid plate assays, we showed that W361R mutation in GaaR results in constitutive production of pectinases. Analysis of subcellular localization of C-terminally eGFP-tagged GaaR/GaaRW 361R revealed important differences in nuclear accumulation of N- versus C-terminally eGFP-tagged GaaR.

PMID: 30298571 [PubMed - in process]




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