BookR: School of Health Core Facilities Booking
PubMed Publications| Keyword search (4,163 papers available) |  |
Concordia Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Phenogenomics reveals the ecology and evolution of Trichoderma fungi for sustainable agriculture | Steindorff AS; Cai FM; Ding M; Jiang S; Atanasova L; Baker SE; Barbosa-Filho JR; Bayram Akcapinar G; Brown DW; Chaverri P; Chen P; Chenthamara K; Daum C; Drula E; Dubey M; Brandström Durling M; Flatschacher D; Ebner T; Emri T; Gao R; Georg RC; Henrissat B; Hermosa R; Herrera-Estrella A; Hinterdobler W; Kainz P; Karlsson M; Kredics L; Kubicek CP; Kuo A; LaButti K; Lipzen A; Lorito M; Mach RL; Manganiello G; Marik T; Martinez-Reyes N; Mayrhofer-Reinhartshuber M; Miskei M; Moisan MC; Mondo S; Monte E; Ng V; Pa | 41775999 GENOMICS |
| 2 | An examination of the quinic acid utilization genes in Aspergillus niger reveals the involvement of two pH-dependent permeases | Sgro M; Reid ID; Arentshorst M; Ram AFJ; Tsang A; | 40853219 GENOMICS |
| 3 | Global survey of secondary metabolism in em Aspergillus niger /em via activation of specific transcription factors | Semper C; Pham TTM; Ram S; Palys S; Evdokias G; Ouedraogo JP; Moisan MC; Geoffrion N; Reid I; Di Falco M; Bailey Z; Tsang A; Benoit-Gelber I; Savchenko A; | 40852424 GENOMICS |
| 4 | Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification | Raheja Y; Singh V; Gaur VK; Sharma G; Tsang A; Chadha BS; | 40622460 GENOMICS |
| 5 | Heterologous Expression of Thermostable Endoglucanases from Rasamsonia emersonii: A Paradigm Shift in Biomass Hydrolysis | Raheja Y; Singh V; Gaur VK; Tsang A; Chadha BS; | 40418313 GENOMICS |
| Title: | Heterologous Expression of Thermostable Endoglucanases from Rasamsonia emersonii: A Paradigm Shift in Biomass Hydrolysis | ||||
| Authors: | Raheja Y, Singh V, Gaur VK, Tsang A, Chadha BS | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/40418313/ | ||||
| DOI: | 10.1007/s12010-025-05258-5 | ||||
| Publication: | Applied biochemistry and biotechnology | ||||
| Keywords: | Rasamsonia emersonii; High thermostability; Hydrolysis; Liquefaction; Pre-treated rice straw; | ||||
| PMID: | 40418313 | Category: | Date Added: | 2025-05-26 | |
| Dept Affiliation: |
GENOMICS
1 Department of Microbiology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India. 2 School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919, Republic of Korea. 3 Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, India. 4 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada. 5 Department of Microbiology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India. chadhabs@yahoo.com. |
||||
Description: |
In this study, two thermostable endoglucanases (Rem_GH5EG and Rem_GH7EG) from Rasamsonia emersonii were heterologously expressed in Pichia pastoris and characterized to evaluate their potential for industrial biomass saccharification. Rem_GH5EG demonstrated markedly superior catalytic efficiency toward barley ß-glucan (kcat/Km = 6.3 × 10-3/mg mL/min), while Rem_GH7EG exhibited a preference for carboxymethyl cellulose (kcat/Km = 1.17 × 10-3/mg mL/min). Notably, Rem_GH5EG showed optimal activity at 90 °C with a half-life (t1/2) of 2 h, whereas Rem_GH7EG was active at 70 °C with a half-life (t1/2) of 1 h, highlighting its suitability for high-temperature hydrolysis processes. Moreover, pre-conditioning of steam and acid pretreated unwashed rice straw slurry with Rem_GH5EG at 90 °C effectively reduced viscosity-related mass transfer limitations, thereby enhancing the hydrolytic efficiency of benchmark cellulase. These findings underscore the industrial relevance of Rem_GH5EG as the more promising candidate for developing efficient enzyme cocktails for biomass saccharification. |
