PERFORM Publications

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Title		Authors	PubMed ID
1	Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation.	Soroko M, Kwan DH	32441090 CHEMBIOCHEM
2	Identification of active site residues of chorismate mutase-prephenate dehydrogenase from Escherichia coli.	Christendat D, Turnbull J	8605196 CHEMBIOCHEM
3	Characterization of active and inactive forms of the phenol hydroxylase stimulatory protein DmpM.	Cadieux E, Powlowski J	10451366 CHEMBIOCHEM
4	S-nitrosation of Ca(2+)-loaded and Ca(2+)-free recombinant calbindin D(28K) from human brain.	Tao L, Murphy ME, English AM	11994015 CHEMBIOCHEM
5	Mechanism of S-nitrosation of recombinant human brain calbindin D28K.	Tao L, English AM	12641465 CHEMBIOCHEM
6	Protein S-glutathiolation triggered by decomposed S-nitrosoglutathione.	Tao L, English AM	15049710 CHEMBIOCHEM
7	Mass spectrometric analysis of nitroxyl-mediated protein modification: comparison of products formed with free and protein-based cysteines.	Shen B, English AM	16229492 CHEMBIOCHEM
8	A shared binding site for NAD+ and coenzyme A in an acetaldehyde dehydrogenase involved in bacterial degradation of aromatic compounds.	Lei Y, Pawelek PD, Powlowski J	18537268 CHEMBIOCHEM
9	Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase η opposite Intrastrand Cross-Linked DNA.	O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ	26624500 CHEMBIOCHEM
10	Proton release due to manganese binding and oxidation in modified bacterial reaction centers.	Kálmán L, Thielges MC, Williams JC, Allen JP	16201752 PHYSICS
11	Light-induced conformational changes in photosynthetic reaction centers: dielectric relaxation in the vicinity of the dimer.	Deshmukh SS, Williams JC, Allen JP, Kálmán L	21141811 PHYSICS
12	Light-induced conformational changes in photosynthetic reaction centers: redox-regulated proton pathway near the dimer.	Deshmukh SS, Williams JC, Allen JP, Kálmán L	21410139 PHYSICS
13	Light-induced conformational changes in photosynthetic reaction centers: impact of detergents and lipids on the electronic structure of the primary electron donor.	Deshmukh SS, Akhavein H, Williams JC, Allen JP, Kalman L	21561160 PHYSICS

Title:	Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation.
Authors:	Soroko M, Kwan DH
Link:	https://www.ncbi.nlm.nih.gov/pubmed/32441090?dopt=Abstract
DOI:	10.1021/acs.biochem.0c00286
Publication:	Biochemistry
Keywords:
PMID:	32441090	Category:	Biochemistry	Date Added:	2020-06-02
Dept Affiliation:	CHEMBIOCHEM 1 Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec, Canada H4B 1R6. 2 Department of Biology, Centre for Applied Synthetic Biology, and Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec, Canada H4B 1R6.

Description:

Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation.

Biochemistry. 2020 Jun 01;:

Authors: Soroko M, Kwan DH

Abstract

We report a straightforward enzymatic synthesis of the 4-methylumbelliferyl glycoside of a complex-type oligosaccharide substrate for core fucosylation. We demonstrate the use of this synthetic glycoconjugate in a newly developed enzyme assay to probe the activity and inhibition of fucosyltransferase VIII, which catalyzes the core fucosylation of N-glycans on eukaryotic glycoproteins. In this fucosyltransferase assay, we use the fluorogenic probe and a specific glycosidase in a sequentially coupled enzyme reaction to distinguish an unmodified 4-methylumbelliferyl oligosaccharide probe from a fucosylated probe. Our findings show that this strategy is very sensitive and specific in its detection of enzyme activity and can even be used for analyzing impure tissue lysate samples.

PMID: 32441090 [PubMed - as supplied by publisher]

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