Keyword search (4,163 papers available)

"Zheng Y" Authored Publications:

Title Authors PubMed ID
1 Associations between brain and cognitive resilience, tau load and extent in Alzheimer's disease Mitchell SW; Chan T; Trudel L; Hosseini SA; Macedo AC; Gonçalves MP; Rahmouni N; Hall BJ; Socualaya KMQ; Therriault J; Servaes S; Bezgin G; Zheng Y; Aumont E; Wang YT; Arias JF; Real APB; Jia WL; Hopewell R; Hsiao C; Soucy JP; Vitali P; Pascoal TA; Rosa-Neto P; 41512332
PERFORM
2 Alzheimer s Imaging Consortium Mitchell SW; Chan T; Trudel L; Hosseini SA; Macedo AC; Gonçalves MP; Rahmouni N; Hall BJ; Socualaya KMQ; Therriault J; Servaes S; Bezgin G; Zheng Y; Aumont E; Wang YT; Arias JF; Real APB; Jia WL; Hopewell R; Hsiao C; Soucy JP; Vitali P; Pascoal TA; Rosa-Neto P; 41433447
PERFORM
3 Risks of per- and polyfluoroalkyl substance exposure through marine fish consumption Qiu W; Yang G; Cao L; Niu S; Li Y; Fang D; Dong Z; Magnuson JT; Schlenk D; Leung KMY; Zheng Y; Zeng Z; Feng L; Zhang X; Zhang Y; Fan W; Huang T; Ma J; Wu M; Tao S; Zheng C; 41411415
CHEMBIOCHEM
4 Integrative approach to mitigate chromium toxicity in soil and enhance antioxidant activities in rice (Oryza sativa L.) using magnesium-iron nanocomposite and Staphylococcus aureus strains Ali MA; Sardar MF; Dar AA; Niaz M; Ali J; Wang Q; Zheng Y; Luo Y; Albasher G; Li F; 39190219
ENCS
5 Carbohydrate esterase family 16 contains fungal hemicellulose acetyl esterases (HAEs) with varying specificity Venegas FA; Koutaniemi S; Langeveld SMJ; Bellemare A; Chong SL; Dilokpimol A; Lowden MJ; Hilden KS; Leyva-Illades JF; Mäkelä MR; My Pham TT; Peng M; Hancock MA; Zheng Y; Tsang A; Tenkanen M; Powlowski J; de Vries RP; 35405333
CSFG
6 Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor. Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L 17333176
BIOLOGY
7 Development of a pyrG mutant of Aspergillus oryzae strain S1 as a host for the production of heterologous proteins. Ling SO, Storms R, Zheng Y, Rodzi MR, Mahadi NM, Illias RM, Abdul Murad AM, Abu Bakar FD 24381522
BIOLOGY
8 Enhancement of synthetic Trichoderma-based enzyme mixtures for biomass conversion with an alternative family 5 glycosyl hydrolase from Sporotrichum thermophile. Ye Z, Zheng Y, Li B, Borrusch MS, Storms R, Walton JD 25295862
CSFG

 

Title:Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor.
Authors:Lahjouji KStorms RXiao ZJoung KBZheng YPowlowski JTsang AVarin L
Link:https://www.ncbi.nlm.nih.gov/pubmed/17333176?dopt=Abstract
DOI:10.1007/s00253-006-0824-5
Publication:Applied microbiology and biotechnology
Keywords:
PMID:17333176 Category:Appl Microbiol Biotechnol Date Added:2019-06-20
Dept Affiliation: BIOLOGY
1 Centre for Structural and Functional Genomics, Biology Department, Concordia University, 7141 Sherbrooke street West, Montréal, Quebec, H4B 1R6, Canada.

Description:

Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor.

Appl Microbiol Biotechnol. 2007 May;75(2):337-46

Authors: Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L

Abstract

A cellobiohydrolase-encoding cDNA, Tvcel7a, from Trametes versicolor has been cloned and expressed in Aspergillus niger. The deduced amino acid sequence shows that Tvcel7a encodes a 456-amino acid polypeptide belonging to glycosyl hydrolase family 7. TvCel7a possesses a 19-amino acid secretion signal but does not possess a linker region nor a carbohydrate-binding domain. Two peaks of activity were obtained after TvCel7a was purified to apparent homogeneity by gel-filtration followed by anion-exchange chromatography. Mass spectrometry performed on the purified proteins confirmed that both peaks corresponded to the predicted sequence of the T. versicolor cellulase. The biochemical properties of the purified TvCel7a obtained from both peaks were studied in detail. The pH and temperature optima were 5.0 and 40 degrees C, respectively. The enzyme was stable over a pH range extending from pH 3.0 to 9.0 and at temperatures lower than 50 degrees C. The kinetic parameters with the substrate p-nitrophenyl beta-D: -cellobioside (pNPC) were 0.58 mM and 1.0 micromol/min/mg protein for the purified TvCel7a found in both peaks 1 and 2. TvCel7a catalyzes the hydrolysis of pNPC, filter paper, beta-glucan, and avicel to varying extents, but no detectable hydrolysis was observed when using the substrates carboxymethylcellulose, laminarin and pNPG.

PMID: 17333176 [PubMed - indexed for MEDLINE]





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