Keyword search (4,164 papers available)

"U S" Authored Publications:

Title Authors PubMed ID
1 Individual differences in empathy-related responses in early childhood: A person-centred approach Bullinger J; Christner N; Urian R; Kellermann CM; Beaulieu S; Steinbeis N; Dunfield KA; Paulus M; 41888065
PSYCHOLOGY
2 Capacitive bimetallic redox cycles and ligand-to-metal charge transfer to Boost denitrification with Ni sup II /sup /Fe sup II /sup -Gallic acid phenolic networks Yu S; Jin Y; Guo T; Li H; Liu W; Chen Z; Wang X; Guo J; 41707775
ENCS
3 Clinical Manifestations Marinou S; Phillips NA; 41448190
CONCORDIA
4 Risks of per- and polyfluoroalkyl substance exposure through marine fish consumption Qiu W; Yang G; Cao L; Niu S; Li Y; Fang D; Dong Z; Magnuson JT; Schlenk D; Leung KMY; Zheng Y; Zeng Z; Feng L; Zhang X; Zhang Y; Fan W; Huang T; Ma J; Wu M; Tao S; Zheng C; 41411415
CHEMBIOCHEM
5 Manganese-Based Spinel Cathodes: A Promising Frontier for Solid-State Lithium-Ion Batteries Dou Y; Zhou S; Dawkins JIG; Zaghib K; Amine K; Xu GL; Deng S; 41137442
ENCS
6 A dataset from a coordinated multi-site laboratory study investigating the Hue-Heat-Hypothesis Bavaresco M; Cureau RJ; Pigliautile I; Barna E; Deme Belafi Z; Belussi L; Chinazzo G; Chiucchiù A; Danza L; Deng Z; Dong B; Gapski NH; Garlet L; Gnecco VM; Guo X; Pilehchi Ha P; Karimian H; Lamberts R; Liu S; da Costa Loeser B; Massucci C; Melo AP; Nagy BV; Ouf MM; Salamone F; Schweiker M; Pisello AL; 40973711
ENCS
7 Engineered iron-sulfur carriers for efficient mixotrophic and sulfur autotrophic denitrification in low carbon to nitrogen ratio municipal wastewater: Mechanisms of biofilm enhancement and electron transfer promotion Yu S; Zhang X; Guo T; Li H; Liu W; Chen Z; Wang X; Ren B; Guo J; 40712941
ENCS
8 Hemodynamic correlates of fluctuations in neuronal excitability: A simultaneous Paired Associative Stimulation (PAS) and functional near infra-red spectroscopy (fNIRS) study Cai Z; Pellegrino G; Spilkin A; Delaire E; Uji M; Abdallah C; Lina JM; Fecteau S; Grova C; 40567300
PERFORM
9 Elucidating the size distribution of p‑Phenylenediamine-Derived quinones in atmospheric particles Xia K; Qin M; Han M; Zhang X; Wu X; Liu M; Liu S; Wang X; Liu W; Xie Z; Yuan R; Liu Q; 39978217
CHEMBIOCHEM
10 Two-dimensional Nanosheets by Liquid Metal Exfoliation Bai Y; Xu Y; Sun L; Ward Z; Wang H; Ratnayake G; Wang C; Zhao M; He H; Gao J; Wu M; Lu S; Bepete G; Peng D; Liu B; Kang F; Terrones H; Terrones M; Lei Y; 39707650
PHYSICS
11 Oil spills in coastal regions of the Arctic and Subarctic: Environmental impacts, response tactics, and preparedness Bi H; Wang Z; Yue R; Sui J; Mulligan CN; Lee K; Pegau S; Chen Z; An C; 39689468
ENCS
12 Peripheral Arterial Disease in Nova Scotia: Increased Prevalence, Low Public Awareness, and Poor Edinburgh Claudication Questionnaire Sensitivity MacLean EA; Fogarty EJ; Peterson BJ; Xu S; Giacomantonio NB; 39525342
CONCORDIA
13 An integrated environmental and economic assessment for the disposal of food waste from grocery retail stores towards resource recovery Zhou S; Chen Z; Huang X; Yang X; Lyu L; An C; Peng H; 39480576
ENCS
14 Supporting parent capacity to manage pain in young children with cancer at home: Co-design and usability testing of the PainCaRe app Jibb LA; Liu W; Stinson JN; Nathan PC; Chartrand J; Alberts NM; Hashemi E; Masama T; Pease HG; Torres LB; Cortes HG; Kuczynski S; Liu S; La H; Fortier MA; 39473834
CONCORDIA
15 Evaluating Sustainable Practices for Managing Residue Derived from Wheat Straw Shanmugam H; Raghavan V; Rajagopal R; Goyette B; Lyu L; Zhou S; An C; 38927790
ENCS
16 Spotlight on the vertical migration of aged microplastics in coastal waters Yang X; Huang G; Chen Z; Feng Q; An C; Lyu L; Bi H; Zhou S; 38503206
ENCS
17 The First Iranian Case of Unstable Hemoglobin Santa Ana Alavi S; Mohammadimoghaddam S; Najmabadi H; Maghsoudlou S; 38500334
ENCS
18 Silica-coated LiYF4:Yb3+, Tm3+ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells Bietar K; Chu S; Mandl G; Zhang E; Chabaytah N; Sabelli R; Capobianco JA; Stochaj U; 38495986
CNSR
19 Hydrothermal synthesis and electrochemical properties of Sn-based peanut shell biochar electrode materials Wang Y; Wang H; Ji J; You T; Lu C; Liu C; Song Y; Chen Z; Zhu S; 38380232
ENCS
20 Unveiling the Vertical Migration of Microplastics with Suspended Particulate Matter in the Estuarine Environment: Roles of Salinity, Particle Properties, and Hydrodynamics Yang X; Huang G; Feng Q; An C; Zhou S; Bi H; Lyu L; 38306690
ENCS
21 Augmenting glutamatergic, but not dopaminergic, activity in the nucleus accumbens shell disrupts responding to a discrete alcohol cue in an alcohol context Valyear MD; Brown A; Deyab G; Villaruel FR; Lahlou S; Caporicci-Dinucci N; Chaudhri N; 38185906
PSYCHOLOGY
22 TRAPPC6B biallelic variants cause a neurodevelopmental disorder with TRAPP II and trafficking disruptions Almousa H; Lewis SA; Bakhtiari S; Nordlie SH; Pagnozzi A; Magee H; Efthymiou S; Heim JA; Cornejo P; Zaki MS; Anwar N; Maqbool S; Rahman F; Neilson DE; Vemuri A; Jin SC; Yang XR; Heidari A; van Gassen K; Trimouille A; Thauvin-Robinet C; Liu J; Bruel AL; Tomoum H; Shata MO; Hashem MO; Toosi MB; Ghayoor Karimiani E; Yesil G; Lingappa L; Baruah D; Ebrahimzadeh F; Van-Gils J; Faivre L; Zamani M; Galehdari H; Sadeghian S; Shariati G; Mohammad R; van der Smagt J; Qari A; Vincent JB; Innes AM; Dursun A; Özgül RK; A 37713627
BIOLOGY
23 Photonic enhancement in photoluminescent metal halide perovskite-photonic crystal bead hybrids Lapointe V; Imperiale CJ; Chengadu S; Pomilio CM; Ganesh M; Kéna-Cohen S; Majewski MB; 37548908
PHYSICS
24 Employee human resource management values: validation of a new concept and scale Drouin-Rousseau S; Fernet C; Austin S; Fabi B; Morin AJS; 37213377
CONCORDIA
25 A Small Molecule Inhibitor of Erg251 Makes Fluconazole Fungicidal by Inhibiting the Synthesis of the 14α-Methylsterols Lu H; Li W; Whiteway M; Wang H; Zhu S; Ji Z; Feng Y; Yan L; Fang T; Li L; Ni T; Zhang X; Lv Q; Ding Z; Qiu L; Zhang D; Jiang Y; 36475771
BIOLOGY
26 Atorvastatin lowers serum calcium levels in lithium-users: results from a randomized controlled trial Soh JF; Bodenstein K; Yu OHY; Linnaranta O; Renaud S; Mahdanian A; Su CL; Mucsi I; Mulsant B; Herrmann N; Rajji T; Beaulieu S; Sekhon H; Rej S; 36153583
PSYCHOLOGY
27 Multi-label classification for biomedical literature: an overview of the BioCreative VII LitCovid Track for COVID-19 literature topic annotations Chen Q; Allot A; Leaman R; Islamaj R; Du J; Fang L; Wang K; Xu S; Zhang Y; Bagherzadeh P; Bergler S; Bhatnagar A; Bhavsar N; Chang YC; Lin SJ; Tang W; Zhang H; Tavchioski I; Pollak S; Tian S; Zhang J; Otmakhova Y; Yepes AJ; Dong H; Wu H; Dufour R; Labrak Y; Chatterjee N; Tandon K; Laleye FAA; Rakotoson L; Chersoni E; Gu J; Friedrich A; Pujari SC; Chizhikova M; Sivadasan N; Vg S; Lu Z; 36043400
ENCS
28 Acute food deprivation-induced relapse to heroin seeking after short and long punishment-imposed abstinence in male rats Borges C; Inigo F; Quteishat N; Charles J; Ah-Yen E; U S; 35951079
CSBN
29 Microfluidic Platforms for the Isolation and Detection of Exosomes: A Brief Review Raju D; Bathini S; Badilescu S; Ghosh A; Packirisamy M; 35630197
ENCS
30 Relationship Between Actual and Perceived Locomotor and Ball Skills Competence Among Children: A Person-Centered Approach Maïano C; Morin AJS; April J; Coutu S; Dugas C; Hue O; 34636286
PSYCHOLOGY
31 Group Cognitive Behavioral Analysis System of Psychotherapy (CBASP): A Pilot Study for Bipolar Depression. Sayegh L; Touré EH; Farquhar E; Beaulieu S; Renaud S; Rej S; Perreault M; 33173513
CONCORDIA
32 Gold Nano-Island Platforms for Localized Surface Plasmon Resonance Sensing: A Short Review. Badilescu S, Raju D, Bathini S, Packirisamy M 33066088
ENCS
33 Dissociable mesolimbic dopamine circuits control responding triggered by alcohol-predictive discrete cues and contexts. Valyear MD, Glovaci I, Zaari A, Lahlou S, Trujillo-Pisanty I, Andrew Chapman C, Chaudhri N 32724058
PSYCHOLOGY
34 Inactograms and objective sleep measures as means to capture subjective sleep problems in patients with a bipolar disorder. Lavin-Gonzalez P, Bourguignon C, Crescenzi O, Beaulieu S, Storch KF, Linnaranta O 32232937
PSYCHOLOGY
35 Correction to: Differential disruption of conditioned ejaculatory preference in the male rat based on different sensory modalities by micro-infusions of naloxone to the medial preoptic area or ventral tegmental area. Quintana GR, Birrel M, Marceau S, Kalantari N, Bowden J, Bachoura Y, Borduas E, Lemay V, Payne JW, Cionnaith CM, Pfaus JG 31919562
PSYCHOLOGY
36 Lab-On-A-Chip for the Development of Pro-/Anti-Angiogenic Nanomedicines to Treat Brain Diseases. Subramaniyan Parimalam S, Badilescu S, Sonenberg N, Bhat R, Packirisamy M 31817343
ENCS
37 Nano-Bio Interactions of Extracellular Vesicles with Gold Nanoislands for Early Cancer Diagnosis. Bathini S, Raju D, Badilescu S, Kumar A, Ouellette RJ, Ghosh A, Packirisamy M 31549028
ENCS
38 Non-invasive in vivo hyperspectral imaging of the retina for potential biomarker use in Alzheimer's disease. Hadoux X, Hui F, Lim JKH, Masters CL, Pébay A, Chevalier S, Ha J, Loi S, Fowler CJ, Rowe C, Villemagne VL, Taylor EN, Fluke C, Soucy JP, Lesage F, Sylvestre JP, Rosa-Neto P, Mathotaarachchi S, Gauthier S, Nasreddine ZS, Arbour JD, Rhéaume MA, Beaulieu S, Dirani M, Nguyen CTO, Bui BV, Williamson R, Crowston JG, van Wijngaarden P 31530809
PERFORM
39 The NSERC Canadian Lake Pulse Network: A national assessment of lake health providing science for water management in a changing climate. Huot Y, Brown CA, Potvin G, Antoniades D, Baulch HM, Beisner BE, Bélanger S, Brazeau S, Cabana H, Cardille JA, Del Giorgio PA, Gregory-Eaves I, Fortin MJ, Lang AS, Laurion I, Maranger R, Prairie YT, Rusak JA, Segura PA, Siron R, Smol JP, Vinebrooke RD, Walsh DA 31419692
BIOLOGY
40 Differential disruption of conditioned ejaculatory preference in the male rat based on different sensory modalities by micro-infusions of naloxone to the medial preoptic area or ventral tegmental area. Quintana GR, Birrel M, Marceau S, Kalantari N, Bowden J, Bachoura Y, Borduas E, Lemay V, Payne JW, Cionnaith CM, Pfaus JG 31359118
PSYCHOLOGY
41 The time-varying effect of radiotherapy after breast-conserving surgery for DCIS. Rakovitch E, Sutradhar R, Hallett M, Thompson AM, Gu S, Dumeaux V, Whelan TJ, Paszat L 31368035
PERFORM
42 TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment. Milev MP, Hasaj B, Saint-Dic D, Snounou S, Zhao Q, Sacher M 25918224
BIOLOGY
43 A molecular phylogeny of thermophilic fungi. Morgenstern I, Powlowski J, Ishmael N, Darmond C, Marqueteau S, Moisan MC, Quenneville G, Tsang A 22483047
CSFG
44 Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila. Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A 24881579
BIOLOGY
45 Phylogeny reconstruction and hybrid analysis of populus (Salicaceae) based on nucleotide sequences of multiple single-copy nuclear genes and plastid fragments. Wang Z, Du S, Dayanandan S, Wang D, Zeng Y, Zhang J 25116432
BIOLOGY
46 mycoCLAP, the database for characterized lignocellulose-active proteins of fungal origin: resource and text mining curation support. Strasser K, McDonnell E, Nyaga C, Wu M, Wu S, Almeida H, Meurs MJ, Kosseim L, Powlowski J, Butler G, Tsang A 25754864
CSFG
47 Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing. Bellemare A, John T, Marqueteau S 29876805
CSFG
48 Tuning of Morphology and Stability of Gold Nanostars Through pH Adjustment. Kumar R, Badilescu S, Packirisamy M 30913757
ENCS
49 Phylogenetic Analysis of Protein Family. Song L, Wu S, Tsang A 29876824
CSFG
50 The effect of hydrogen nanobubbles on the morphology of gold-gelatin bionanocomposite films and their optical properties. Alsawafta M, Badilescu S, Truong VV, Packirisamy M 22248640
PHYSICS
51 Laser-induced micropore formation and modification of cartilage structure in osteoarthritis healing. Sobol E, Baum O, Shekhter A, Wachsmann-Hogiu S, Shnirelman A, Alexandrovskaya Y, Sadovskyy I, Vinokur V 28564689
PHYSICS
52 Self-concept research with school-aged youth with intellectual disabilities: A systematic review. Maïano C, Coutu S, Morin AJS, Tracey D, Lepage G, Moullec G 30515961
PSYCHOLOGY
53 Modeling Relapse to Pavlovian Alcohol-Seeking in Rats Using Reinstatement and Spontaneous Recovery Paradigms. LeCocq MR, Lahlou S, Chahine M, Padillo LN, Chaudhri N 29969151
CSBN
54 Relationships between lower body strength and the energy cost of treadmill walking in a cohort of healthy older adults: a cross-sectional analysis. Berryman N, Bherer L, Nadeau S, Lauzière S, Lehr L, Bobeuf F, Kergoat MJ, Vu TT, Bosquet L 27815704
PERFORM

 

Title:Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing.
Authors:Bellemare AJohn TMarqueteau S
Link:https://www.ncbi.nlm.nih.gov/pubmed/29876805?dopt=Abstract
DOI:10.1007/978-1-4939-7804-5_2
Publication:Methods in molecular biology (Clifton, N.J.)
Keywords:Agarose gel electrophoresisColony PCRDNA quantificationFluorometryFungal genomic DNA extractionMyceliaSpectrophotometer
PMID:29876805 Category:Methods Mol Biol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada. annie.bellemare@concordia.ca.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada.

Description:

Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing.

Methods Mol Biol. 2018;1775:11-20

Authors: Bellemare A, John T, Marqueteau S

Abstract

Isolation of fungal genomic DNA of high quality is required for a number of downstream biotechnology-derived applications such as genome sequencing, microarrays, and digital PCR technologies, to only name a few. In most cases, not only a high molecular weight DNA of superior grade is required but also large quantities. On the other hand, a number of laboratory experiments, such as polymerase chain reaction (PCR) for medical diagnostic or for genotyping, have to be conducted in a limited amount of time and can provide complete results with the use of lower quality DNA. We describe here two different fungal DNA extraction approaches, which are applicable to a wide range of fungal species.First, we adapted a DNA extraction method for PCR-based genotyping which allows analysis of single to hundreds of colonies simultaneously. Cells are disrupted in the presence of sodium dodecyl sulfate and Proteinase K which are then removed by precipitation and centrifugation. The cleared lysate is used for PCR reaction.Secondly, we describe a method to obtain genome sequencing quality grade DNA from fungal liquid cultures. Mycelia are harvested by either filtration or centrifugation. Cells are mechanically disrupted by liquid nitrogen grinding, followed by genomic DNA extraction using the QIAGEN's DNeasy® Plant Kit. The quality and quantity of genomic DNA is monitored by fluorometry.

PMID: 29876805 [PubMed - indexed for MEDLINE]





BookR developed by Sriram Narayanan
for the Concordia University School of Health
Copyright © 2011-2026
Cookie settings
Concordia University