PERFORM Publications

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Title		Authors	PubMed ID
1	Nonphotochemical Spectral Hole Burning Study of Modified LH2 Complex from em Rbl. acidophilus /em : Do Parts of the Pigment Molecule Affect Small Light-Induced Structural Changes?	Levenberg A; Trempe A; Lujan MA; Picorel R; Zazubovich V;	41802209 PHYSICS
2	Evidence of Simultaneous Spectral Hole Burning Involving Two Tiers of the Protein Energy Landscape in Cytochrome b6f.	Shafiei G, Levenberg A, Lujan MA, Picorel R, Zazubovich V	31763829 PHYSICS
3	Parameters of the protein energy landscapes of several light-harvesting complexes probed via spectral hole growth kinetics measurements.	Herascu N, Najafi M, Amunts A, Pieper J, Irrgang KD, Picorel R, Seibert M, Zazubovich V	21391534 PHYSICS
4	Effects of the distributions of energy or charge transfer rates on spectral hole burning in pigment-protein complexes at low temperatures.	Herascu N, Ahmouda S, Picorel R, Seibert M, Jankowiak R, Zazubovich V	22046956 PHYSICS
5	Spectral hole burning, recovery, and thermocycling in chlorophyll-protein complexes: distributions of barriers on the protein energy landscape.	Najafi M, Herascu N, Seibert M, Picorel R, Jankowiak R, Zazubovich V	22957798 PHYSICS
6	Conformational Changes in Pigment-Protein Complexes at Low Temperatures-Spectral Memory and a Possibility of Cooperative Effects.	Najafi M, Herascu N, Shafiei G, Picorel R, Zazubovich V	25985255 PHYSICS
7	A simple and efficient method to prepare pure dimers and monomers of the cytochrome b 6 f complex from spinach.	Luján MA, Lorente P, Zazubovich V, Picorel R	28374305 PHYSICS
8	Probing Energy Landscapes of Cytochrome b6f with Spectral Hole Burning: Effects of Deuterated Solvent and Detergent.	Levenberg A, Shafiei G, Lujan MA, Giannacopoulos S, Picorel R, Zazubovich V	28956922 PHYSICS

Title:	A simple and efficient method to prepare pure dimers and monomers of the cytochrome b 6 f complex from spinach.
Authors:	Luján MA, Lorente P, Zazubovich V, Picorel R
Link:	https://www.ncbi.nlm.nih.gov/pubmed/28374305?dopt=Abstract
Publication:
Keywords:
PMID:	28374305	Category:	Photosynth Res	Date Added:	2019-06-04
Dept Affiliation:	PHYSICS 1 CSIC-Estación Experimental de Aula Dei, Avda. Montañana 1005, 50059, Zaragoza, Spain. 2 Department of Physics, Concordia University, 7141 Sherbrook Str. West, Montreal, QC, H4B 1R6, Canada. 3 CSIC-Estación Experimental de Aula Dei, Avda. Montañana 1005, 50059, Zaragoza, Spain. picorel@eead.csic.es.

Description:

A simple and efficient method to prepare pure dimers and monomers of the cytochrome b 6 f complex from spinach.

Photosynth Res. 2017 Jun;132(3):305-309

Authors: Luján MA, Lorente P, Zazubovich V, Picorel R

Abstract

Using a single size-exclusion chromatography we were able to isolate highly pure dimers and monomers of the Cyt b 6 f complex from spinach from a bulk preparation of that protein complex obtained with a standard procedure. At higher protein/detergent ratio during the chromatography most of the Cyt b 6 f complex remained as dimers. In contrast, at lower protein/detergent ratio (around 15 times lower), most dimers became monomerized. As a bonus, this chromatography also allowed the elimination of potential Chl a contaminant to the Cyt b 6 f preparations. SDS-PAGE protein analysis with 18% (w/v) acrylamide revealed the loss of the ISP subunit in our monomeric preparation. However, it fully retained the content of Chl a, a prerequisite to perform any spectroscopic study involving this unique pigment.

PMID: 28374305 [PubMed - indexed for MEDLINE]

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