Keyword search (4,163 papers available)

"Ouedraogo JP" Authored Publications:

Title Authors PubMed ID
1 Global survey of secondary metabolism in em Aspergillus niger /em via activation of specific transcription factors Semper C; Pham TTM; Ram S; Palys S; Evdokias G; Ouedraogo JP; Moisan MC; Geoffrion N; Reid I; Di Falco M; Bailey Z; Tsang A; Benoit-Gelber I; Savchenko A; 40852424
GENOMICS
2 The effects of external Mn2+ concentration on hyphal morphology and citric acid production are mediated primarily by the NRAMP-family transporter DmtA in Aspergillus niger. Fejes B, Ouedraogo JP, Fekete E, Sándor E, Flipphi M, Soós Á, Molnár ÁP, Kovács B, Kubicek CP, Tsang A, Karaffa L 32000778
CSFG
3 A set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in Aspergillus niger. Niu J, Arentshorst M, Seelinger F, Ram AF, Ouedraogo JP 27251039
CSFG
4 Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger. Song L, Ouedraogo JP, Kolbusz M, Nguyen TTM, Tsang A 30142205
CSFG

 

Title:A set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in Aspergillus niger.
Authors:Niu J,  Arentshorst M,  Seelinger F,  Ram AF,  Ouedraogo JP
Link:https://www.ncbi.nlm.nih.gov/pubmed/27251039?dopt=Abstract
DOI:10.1007/s00203-016-1240-6
Publication:Archives of microbiology
Keywords:Auxotrophy;  Isogenic strains;  Multiple markers;  Parasexual crossing; 
PMID:27251039 Category:Arch Microbiol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands.
2 Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands. a.f.j.ram@biology.leidenuniv.nl.
3 Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke St. W., Montreal, QC, H4B 1R6, Canada.

Description:

A set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in Aspergillus niger.

Arch Microbiol. 2016 Nov;198(9):861-8

Authors: Niu J, Arentshorst M, Seelinger F, Ram AF, Ouedraogo JP

Abstract

To construct a set of isogenic auxotrophic strains in Aspergillus niger suited for creating multiple gene deletion mutants and executing parasexual crossings, we have combined mutations in genes involved in colour pigmentation (fwnA and olvA) with well-selectable auxotrophic markers (pyrG, nicB, argB, and adeA). All markers, except for the pyrG marker, were introduced by targeted deletion, omitting UV mutagenesis of the strains. Aspergillus oryzae orthologous genes of the argB, nicB, and adeA markers were used as heterologous selection markers, and all markers were shown to complement to respective auxotrophic A. niger mutants. A quadruple auxotrophic marker was further constructed suitable for multiple gene deletions. Genome sequencing of two auxotrophic colour mutants JN3.2 (olvA::pyrG, argB::hygB) and JN6.2 (olvA::pyrG, nicB::hygB) revealed four SNPs between them in non-coding regions, indicating a high level of isogenicity between both strains. The availability of near-isogenic complementary auxotrophic colour mutants facilitates the selection of diploids and the isolation of haploid segregants from the diploid using the parasexual cycle.

PMID: 27251039 [PubMed - indexed for MEDLINE]




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