Keyword search (4,163 papers available)

"O'Flaherty DK" Authored Publications:

Title Authors PubMed ID
1 O4-alkyl-2'-deoxythymidine cross-linked DNA to probe recognition and repair by O6-alkylguanine DNA alkyltransferases. McManus FP, O'Flaherty DK, Noronha AM, Wilds CJ 22850722
CHEMBIOCHEM
2 Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase η opposite Intrastrand Cross-Linked DNA. O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ 26624500
CHEMBIOCHEM
3 O(6)-Alkylguanine DNA Alkyltransferase Repair Activity Towards Intrastrand Cross-Linked DNA is Influenced by the Internucleotide Linkage. O'Flaherty DK, Wilds CJ 26692563
CHEMISTRY
4 Lesion Orientation of O4-Alkylthymidine Influences Replication by Human DNA Polymerase η. O'Flaherty DK, Patra A, Su Y, Guengerich FP, Egli M, Wilds CJ 27574558
CHEMBIOCHEM
5 Preparation of Intrastrand {G}O(6) -Alkylene-O(6) {G} Cross-Linked Oligonucleotides. O'Flaherty DK, Wilds CJ 27584704
CHEMBIOCHEM
6 O6-2'-Deoxyguanosine-butylene-O6-2'-deoxyguanosine DNA Interstrand Cross-Links Are Replication-Blocking and Mutagenic DNA Lesions. Xu W, Kool D, O'Flaherty DK, Keating AM, Sacre L, Egli M, Noronha A, Wilds CJ, Zhao L 27768841
CHEMBIOCHEM
7 Site-specific covalent capture of human O6-alkylguanine-DNA-alkyltransferase using single-stranded intrastrand cross-linked DNA. O'Flaherty DK, Wilds CJ 27886318
CHEMBIOCHEM
8 Structural basis of interstrand cross-link repair by O6-alkylguanine DNA alkyltransferase. Denisov AY, McManus FP, O'Flaherty DK, Noronha AM, Wilds CJ 28937154
CHEMBIOCHEM
9 AGT Activity Towards Intrastrand Crosslinked DNA is Modulated by the Alkylene Linker. O'Flaherty DK, Wilds CJ 28980757
CHEMBIOCHEM
10 Altering Residue 134 Confers an Increased Substrate Range of Alkylated Nucleosides to the E. coli OGT Protein. Schoonhoven NM, O'Flaherty DK, McManus FP, Sacre L, Noronha AM, Kornblatt MJ, Wilds CJ 29137116
CHEMBIOCHEM
11 Covalent capture of OGT's active site using engineered human-E. coli chimera and intrastrand DNA cross-links. Copp W, O'Flaherty DK, Wilds CJ 30430154
CHEMBIOCHEM

 

Title:O(6)-Alkylguanine DNA Alkyltransferase Repair Activity Towards Intrastrand Cross-Linked DNA is Influenced by the Internucleotide Linkage.
Authors:O'Flaherty DK,  Wilds CJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/26692563?dopt=Abstract
DOI:10.1002/asia.201501253
Publication:Chemistry, an Asian journal
Keywords:DNA damage;  DNA repair;  O6-alkylguanine DNA alkyltransferase;  intrastrand cross-link;  oligonucleotides; 
PMID:26692563 Category:Chem Asian J Date Added:2019-06-20
Dept Affiliation: CHEMISTRY
1 Department of Chemistry&Biochemistry, Concordia University, 7141 Sherbrooke St. West, Montréal, Québec (Canada.
2 Department of Chemistry&Biochemistry, Concordia University, 7141 Sherbrooke St. West, Montréal, Québec (Canada. Chris.Wilds@concordia.ca.

Description:

O(6)-Alkylguanine DNA Alkyltransferase Repair Activity Towards Intrastrand Cross-Linked DNA is Influenced by the Internucleotide Linkage.

Chem Asian J. 2016 Feb 18;11(4):576-83

Authors: O'Flaherty DK, Wilds CJ

Abstract

Oligonucleotides containing an alkylene intrastrand cross-link (IaCL) between the O(6) -atoms of two consecutive 2'-deoxyguanosines (dG) were prepared by solid-phase synthesis. UV thermal denaturation studies of duplexes containing butylene and heptylene IaCL revealed a 20?°C reduction in stability compared to the unmodified duplexes. Circular dichroism profiles of these IaCL DNA duplexes exhibited signatures consistent with B-form DNA. Human O(6) -alkylguanine DNA alkyltransferase (hAGT) was capable of repairing both IaCL containing duplexes with slightly greater efficiency towards the heptylene analog. Interestingly, repair efficiencies of hAGT towards these IaCL were lower compared to O(6) -alkylene linked IaCL lacking the 5'-3'-phosphodiester linkage between the connected 2'-deoxyguanosine residues. These results demonstrate that the proficiency of hAGT activity towards IaCL at the O(6) -atom of dG is influenced by the backbone phosphodiester linkage between the cross-linked residues.

PMID: 26692563 [PubMed - indexed for MEDLINE]




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